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The process of calcification during development of the rat tracheal cartilage characterized by distribution of alkaline phosphatase activity and immunolocalization of types I and II collagens and glycosaminoglycans of proteoglycans

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Abstract

The rat tracheal cartilage was shown to calcify during development. The process of calcification was characterized in terms of distribution of alkaline phosphatase (ALP) activity and alterations to immunolocalization of types I and II collagens and glycosaminoglycans of proteoglycans during the development of the tracheal cartilage, in comparison with calcification of the epiphyseal growth plate cartilage. ALP activity was not identified in the tracheal cartilage in the course of calcification, which therefore differed from that in the growth plate. The tracheal cartilage matrix was not resorbed or invaded by type I collagen during calcification. This suggests that no osteogenesis is involved in calcification of the cartilage. Immunoreactivity for type II collagen became weaker in the central region of the tracheal cartilage during development. No net loss of proteoglycans was identified with Alcian blue staining after calcification of the tracheal cartilage. Immunoreactivity for chondroitin 4-sulphate increased in the calcified tracheal cartilage, while reactivity for chondroitin 6-sulphate was weaker in the calcified area than in the surrounding uncalcified region of the tracheal cartilage. The alteration of the extracellular matrices during development may be involved in the calcification of the rat tracheal cartilage.

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Authors and Affiliations

  1. 2nd Department of Oral Anatomy, School of Dentistry, Tohoku University, 4-1 Seiryo-Machi, Aoba-Ku, 980, Sendai, Japan

    Yasuyuki Sasano, Mitsuru Furusawa, Nobuhiko Aiba & Manabu Kagayama

  2. Department of Orthodontics, School of Dentistry, Tohoku University, 980, Sendai, Japan

    Itaru Mizoguchi

  3. Department of Endodontics and Periodontics, School of Dentistry, Tohoku University, 980, Sendai, Japan

    Eriko Ohtani

  4. Department of Operative Dentistry, School of Dentistry, Tohoku University, 980, Sendai, Japan

    Yoko Iwamatsu

Authors
  1. Yasuyuki Sasano
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  2. Itaru Mizoguchi
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  3. Mitsuru Furusawa
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  4. Nobuhiko Aiba
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  5. Eriko Ohtani
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  6. Yoko Iwamatsu
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  7. Manabu Kagayama
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Sasano, Y., Mizoguchi, I., Furusawa, M. et al. The process of calcification during development of the rat tracheal cartilage characterized by distribution of alkaline phosphatase activity and immunolocalization of types I and II collagens and glycosaminoglycans of proteoglycans. Anat Embryol 188, 31–39 (1993). https://doi.org/10.1007/BF00191449

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  • DOI: https://doi.org/10.1007/BF00191449

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