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p53 and c-erbB-2 expression in schistosomal urinary bladder carcinomas and schistosomal cystitis with premalignant lesions

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Abstract

Immunoreactivity for p53 and c-erbB-2 proteins was studied in 31 schistosomal urinary bladder carcinomas and 21 cases of schistosomal cystitis with hyperplastic, metaplastic and/or dysplastic (premaligant) lesions. The results were compared with 30 carcinomas and 21 premalignant lesions of the urinary bladder without schistosomiasis. Abnormal nuclear p53 protein accumulation was found in 17/31 schistosomal and in 15/30 non-schistosomal carcinomas and in 8/21 schistosomal cystitis with premalignant lesions of which five showed hyperplasia. No case of non-schistosomal hyperplasia or squamous metaplasia examined showed p53-positivity. In non-schistosomal carcinomas p53 positivity was significantly associated with tumour grade (grade I-II vs grade III tumours: P=0.021) and greater age (P=0.004) while in schistosomal carcinomas no such associations were found. Cytoplasmic membrane-bound positivity for c-erbB-2 oncoprotein was found in comparable percentages in schistosomal and non-schistosomal bladder carcinomas (10%), and in both groups was co-expressed with p53. p53 gene alteration is an important event in the development of both schistosomal and non-schistosomal bladder carcinoma.

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References

  • Akiyama T, Sudo C, Ogawara H, Toyoshima K, Yamamoto T (1986) The product of the human c-erbB-2 gene: a 185-kilo-dalton glycoprotein with tyrosine kinase activity. Science 232: 1644–1646

    Google Scholar 

  • Bartkova J, Bartek J, Lukas J et al. (1991) p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia. Int J Cancer 49: 196–202

    Google Scholar 

  • Brumm C, Riviere A, Wilckens C, Loning T (1990) Immunohistochemical investigation and Northern blot analysis of c-erbB-2 expression in normal, premalignant and malignant tissues of the corpus and cervix uteri. Virchows Arch [A] 417: 477–484

    Google Scholar 

  • Casson AG, Mukhopadhyay T, Cleary KR, Ro JY, Levin B, Roth JA (1991) p53 gene mutations in Barrett's epithelium and esophageal cancer. Cancer Res 51: 4495–4499

    Google Scholar 

  • Dolcetti R, Doglioni C, Maestro R et al. (1992) p53 over-expression is an early event in the development of human squamouscell carcinoma of the larynx: genetic and prognostic implications. Int J Cancer 52: 178–182

    Google Scholar 

  • Eliyahu D, Michalovitz D, Eliyahu S, Pinhasi-Kimhi O, Oren M (1989) Wild-type p53 can inhibit oncogene-mediated focus formation. Proc Natl Acad Sci USA 86: 8763–8767

    Google Scholar 

  • Farmer G, Bargonetti J, Zhu H, Friedman P, Prywes R, Prives C (1992) Wild-type p53 activates transcription in vitro. Nature 358: 83–86

    Google Scholar 

  • Finlay CA, Hinds PW, Levine AJ (1989) The p53 proto-oncogene can act as a suppressor of transformation. Cell 57: 1083–1093

    Google Scholar 

  • Gusterson BA, Anbazhagan R, Warren W et al. (1991) Expression of p53 in premalignant and malignant squamous epithelium. Oncogene 6: 1785–1789

    Google Scholar 

  • Hall PA, Hughes CM, Staddon SL, Richman PI, Gullick WJ, Lemoine NR (1990) The c-erbB-2 proto-oncogene in human pancreatic cancer. J Pathol 161: 195–200

    Google Scholar 

  • Hollstein M, Sidransky D, Vogelstein B, Harris C (1991) p53 mutations in human cancers. Science 253: 49–53

    Google Scholar 

  • Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29: 577–580

    Google Scholar 

  • Iggo R, Gatter K, Bartek J, Lane D, Harris AL (1990) Increased expression of mutant forms of p53 oncogene in primary lung cancer. Lancet 335: 675–679

    Google Scholar 

  • Isola J, Visakorpi T, Holli K, Kallioniemi O-P (1992) Association of overexpression of tumour suppressor protein p53 with rapid cell proliferation and poor prognosis in node-negative breast cancer. J Natl Cancer Inst 84: 1109–1114

    Google Scholar 

  • Kamel D, Pääkkö P, Nuorva K, Vähäkangas K, Soini Y (1993) p53 and c-erbB-2 protein expression in adenocarcinomas and epithelial dysplasias of the gallbladder. J Pathol 170: 67–72

    Google Scholar 

  • Koroltchouk V, Stanley K, Stjernswärd J, Mott K (1987) Bladder cancer: approaches to prevention and control. Bull World Health Organ 65 (4): 513–520

    Google Scholar 

  • Lucas SB (1982) Bladder tumours in Malawi. Br J Urol 54: 275–279

    Google Scholar 

  • Maestro R, Dolceti R, Gasparotto D et al. (1992) High frequency of p53 gene alterations associated with protein overexpression in human squamous cell carcinoma of the larynx. Oncogene 7: 1159–1166

    Google Scholar 

  • McCully RM, Barron CN, Cheever AW (1976) Schistosomiasis (Bilharziasis). In: Binford CH, Connor DH (eds) Pathology of tropical and extra-ordinary diseases. Armed Forces Institute of Pathology, Washington, D.C., pp 482–508

    Google Scholar 

  • Midgley CA, Fisher CJ, Bartek J, Vojtesek B, Lane D, Barnes DM (1992) Expression of human p53 in bacteria: application to the analysis of p53 expression in human tumours. J Cell Sci 101: 183–189

    Google Scholar 

  • Mostofi FK, Sobin LH, Torloni H (1973) Histologic typing of urinary bladder tumours. In: International histological classification of tumours (vol 10). World Health Organization, Geneva

    Google Scholar 

  • Nagy GK, Frable WJ, Murphy MM (1982) Classification of premalignant urothelial abnormalities. A Delphi study of the national bladder cancer collaborative group A. Pathol Annu 17: 219–233

    Google Scholar 

  • Nigro JM, Baker SJ, Preisinger AC et al. (1989) Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705–708

    Google Scholar 

  • Nuorva K, Soini Y, Kamel D et al. (1993) Concurrent p53 expression in bronchial dysplasias and squamous cell lung carcinomas. Am J Pathol 142: 725–732

    Google Scholar 

  • Olumi AF, Tsai YC, Nichols PW et al. (1990) Allelic loss of chromosome 17p distinguishs high grade from low grade transitional cell carcinomas of the bladder. Cancer Res 50: 7081–7083

    Google Scholar 

  • Pääkkö P, Nuorva K, Kamel D, Soini Y (1992) Evidence by in situ hybridization that c-erbB-2 proto-oncogene expression is a marker of malignancy and is expressed in lung adenocarcinomas. Am J Respir Cell Mol Biol 7: 325–334

    Google Scholar 

  • Sato K, Moriyama M, Mori S et al. (1992) An immunohistologic evaluation of c-erbB-2 gene product in patients with urinary bladder carcinoma. Cancer 70: 2493–2498

    Google Scholar 

  • Sidransky D, Von Eschenbach A, Tsai YC et al. (1991) Identification of p53 gene mutations in bladder cancers and urine samples. Science 25: 705–709

    Google Scholar 

  • Singleton TP, Strickler JG (1992) Clinical and pathological significance of the c-erbB-2 (HER-2/neu) oncogene. Pathol Annu (part 1) 27: 164–189

    Google Scholar 

  • Slamon DJ, Godolphin W, Jones LA et al. (1989) Studies of the HER/neu proto-oncogene in human breast and ovarian cancer. Science 244: 707–712

    Google Scholar 

  • Soini Y, Pääkkö P, Nuorva K, Kamel D, Lane DP, Vähäkangas K (1992) Comparative analysis of p53 protein immunoreactivity in prostatic, lung and breast carcinomas. Virchows Arch [A] 421: 223–228

    Google Scholar 

  • Soini Y, Turpeenniemi-Hujanen T, Kamel D et al. (1993) p53 immunohistochemistry in transitional cell carcinoma and dysplasia of the urinary bladder correlates with disease progression. Br J Cancer 68: 1029–1035

    Google Scholar 

  • Soini Y, Mannermaa A, Winqvist R et al. (1994) Application of fine needle aspiration to the demonstration of ERB-B2 and MYC expression by in situ hybridization in breast carcinoma. J Histochem Cytochem 42: (in press)

  • Steinmeyer K, Maacke H, Deppert W (1990) Cell cycle control by p53 in normal (3T3) and chemically transformed (Meth A) mouse cells. I. Regulation of p53 expression. Oncogene 5: 1691–1699

    Google Scholar 

  • Vähäkangas KH, Samet JM, Metcalf RA et al. (1992) Mutations of p53 and ras genes in radon-associated lung cancer from uranium miners. Lancet 339: 576–580

    Google Scholar 

  • Vogelstein B, Kinzler KW (1992) p53 function and dysfunction. Cell 70: 523–526

    Google Scholar 

  • Vojtěšek B, Fisher CJ, Barnes DM, Lane DP (1993) Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique. Br J Cancer 67: 1254–1258

    Google Scholar 

  • Voravud N, Foster CS, Gilbertson JA, Sikora K, Waxman J (1989) Oncogene expression in cholangiocarcinoma and in normal hepatic development. Hum Pathol 20: 1163–1168

    Google Scholar 

  • Wright C, Mellon K, Johnston P et al. (1991) Expression of mutant p53, c-erbB-2 and the epidermal growth factor receptor in transitional cell carcinoma of the human urinary bladder. Br J Cancer 63: 967–970

    Google Scholar 

  • Yamamoto T, Ikawa S, Akiyama T et al. (1986) Similarity of protein encoded by the human c-erbB-2 gene to epidermal growth factor receptor. Nature 319: 230–234

    Google Scholar 

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Kamel, D., Soini, Y., Nuorva, K. et al. p53 and c-erbB-2 expression in schistosomal urinary bladder carcinomas and schistosomal cystitis with premalignant lesions. Vichows Archiv A Pathol Anat 424, 349–355 (1994). https://doi.org/10.1007/BF00190555

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