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In vivo depletion of CD4 and CD8 T lymphocytes impairs Mycobacterium w vaccine-induced protection against M. tubercolosis in mice

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Abstract

In the present study we sought to determine the relative role of CD4 and CD8 T cells in Mycobacterium w-induced protective immunity against tuberculosis of mice by in vivo depletion with specific monoclonal antibodies (mAb). Mice were immunized first with M. w, 4 weeks later treated with anti-CD4, anti-CD8 or a combination of both mAb and subsequently infected with M. tuberculosis H37Rv i.V. Numbers of colony-forming units in animals depleted of CD4 T cells, CD8 T cells or both T cell populations were significantly higher than those in control mice receiving irrelevant mAb or no mAb. Cytokine production by T cell subsets was also determined by culturing the cells remaining after in vivo depletion in the presence or absence of mycobacterial antigens. CD8 (CD4 depleted) T cells produced lower levels of interferon-γ than CD4 (CD8 depleted) T cells. These data suggest that both CD4 and CD8 T cells participate in resistance against tuberculosis induced by vaccination with M. w.

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Authors and Affiliations

  1. Department of Microbiology, National Institute of Immunology, New Delhi, India

    Indira Guleria & Rama Mukherjee

  2. Department of Immunology, University of Ulm, Albert-Einstein-Allee 11, W-7900, Ulm, Germany

    Stefan H. E. Kaufmann

Authors
  1. Indira Guleria
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  2. Rama Mukherjee
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  3. Stefan H. E. Kaufmann
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Guleria, I., Mukherjee, R. & Kaufmann, S.H.E. In vivo depletion of CD4 and CD8 T lymphocytes impairs Mycobacterium w vaccine-induced protection against M. tubercolosis in mice. Med Microbiol Immunol 182, 129–135 (1993). https://doi.org/10.1007/BF00190265

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  • DOI: https://doi.org/10.1007/BF00190265

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