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Generation, characterization and cross-reactivities of monoclonal antibodies against the p24 core protein and the gp130 envelope glycoprotein of HIV-2ben

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Abstract

The purpose of this study was to characterize antigenic determinants on structural polypeptides of human immunodeficiency virus type 2 (HIV-2ben). Therefore, three HIV-2-specific monoclonal antibodies (mAbs) against the p24 core protein (gag) and one mAb against the gp130 envelope glycoprotein (env) were produced. In addition to p24 the anti-core mAbs recognized the primary translation product of the viral gag gene p55 and an intermediate cleavage product p41. Core mAbs cross-reacted with another HIV-2 isolate (HIV-2rod), and several simian immunodeficiency viruses (SIVagm TYO7 and SIVmac), but not with SIVmnd and the HIV-1 isolates investigated (HIV-1han and HIV-1lai). The env mAb cross-reacted with HIV-2rod and SIVmac but not with SIVagm, SIVmnd or HIV-1. In competition assays and with epitope mapping possible binding sites for the mAbs were identified. The processing of HIV-2 core proteins is compared in retrovirus-infected T cell lines and during the expression by recombinant vaccinia virus. Finally, the mAb XIV DC10 which recognized a highly conserved epitope could be useful for an assay to detect HIV-1 and HIV-2 simultaneously. II D8 is the first mAb raised against HIV-2 env glycoprotein.

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References

  1. Clavel F, Guyader M, Guetard D, Salle M, Montagnier L and Alizon M (1986) Molecular cloning and polymorphism of the human immunodeficiency virus type 2. Nature 324:691–695

    Google Scholar 

  2. Dormont D, Livartowski J, Chamaret S (1989) HIV-2 in rhesus monkeys: serological, virological and clinical results. Intervirology 30:59–65

    Google Scholar 

  3. Douillard JY, Hoffman T (1983) Enzyme-linked immunosorbent assay for screening monoclonal antibody production using enzyme labeled second antibody. Methods Enzymol 92:168–174

    Google Scholar 

  4. Fazekas de St. Groth S, Scheidegger D (1980) Production of monoclonal antibodies. Strategy and tactics. J Immunol Methods 32:297–304

    Google Scholar 

  5. Franchini G, Gurgo C, Guo H-G, Gallo RC, Collati E, Fargnoli KA, Hall LF, Wong-Staal F, Reitz MS (1987) Nucleotide sequence of simian immunodeficiency virus and its relationship to the human immunodeficiency viruses. Nature 328:539–543

    Google Scholar 

  6. Friguet B, Djavadi-Ohaniance L, Pages J, Bussard A, Goldberg M (1983) A convenient enzyme-linked immunosorbent assay for testing whether monoclonal antibodies recognize the same antigenic site. J Immunol Methods 60:351–358

    Google Scholar 

  7. Hurn BAL, Chantler SM (1980) Production of reagent antibodies. Methods Enzymol 70:104–142

    Google Scholar 

  8. Kent KA, Gritz L, Stallard G (1991) Production and characterization of monoclonal antibodies to simian immunodeficiency virus envelope glycoproteins. AIDS 5:829–836

    Google Scholar 

  9. Kirchhoff F, Jentsch KD, Stuke A, Mous J, Hunsmann G (1990) Genomic divergence of a HIV-2 from a german AIDS patient probably infected in Mali. AIDS 4:847–857

    Google Scholar 

  10. Kirchhoff F, Voss G, Nick S, Stahl-Hennig C, Coulibali C, Frank R, Jentsch KD, Hunsmann G (1991) Antibody response to the negative regulatory factor (NEF) in experimentally infected macaques: correlation with viremia, disease progression and seroconversion to structural viral proteins. Virology 183:267–272

    Google Scholar 

  11. Klemm E, Schneeweis KE, Horn R, Tackmann W, Schulze G, Schneider J (1988) HIV-2 infection with initial neurologic manifestation. J Neurol 235:304–307

    Google Scholar 

  12. Komatsu H, Tozawa H, Kawamura M, Kodama T, Hayami M (1990) Multiple antigenic epitopes expressed on gag proteins, p24 and p15 of a human immundeficiency virus (HIV) type 2 as defined with a library of monoclonal antibodies. AIDS Res Hurn Retroviruses 6:871–881

    Google Scholar 

  13. Kornfeld H, Riedel N, Viglianti GA, Hirsch V, Mullins JI (1987) Cloning of HTLV-4 and its relationship to simian and human immundeficiency viruses. Nature 326:610–613

    Google Scholar 

  14. Lövgren K, Lindmark J, Pipkorn R, Morein B (1987) Antigenic presentation of small molecules and peptides conjugated to a preformed Iscom as carrier. J Immunol Methods 98:329–356

    Google Scholar 

  15. Minassian AA, Kalyanaraman VS, Gallo RC, Popovic M (1988) Monoclonal antibodies against human immundeficiency virus (HIV) type 2 core proteins: cross-reactivity with HIV type 1 and simian immundeficiency virus. Proc Natl Acad Sci USA 85:6939–6943

    Google Scholar 

  16. Morein B, Sundquist B, Hoglund S, Dalsgaard K, Osterhaus A (1984) Iscom, a novel structure for antigenetic presentation of membrane proteins from enveloped viruses. Nature 308:457–460

    Google Scholar 

  17. Morein B, Lövgren K, Höglund S, Sundquist B (1987) The ISCOM: an immunostimulating complex. Immunol Today 8:333–338

    Google Scholar 

  18. Nicol I, Messinger D, Bubouch P, Bernard J, Desportes I, Jouffre R, Snart R, Nara P, Gallo RC, Zagury D (1989) Use of old world monkeys for acquired immunodeficiency syndrome research. J Med Primatol 18:227–236

    Google Scholar 

  19. Otteken A, Nick S, Bergter W, Voss G, Faisst AC, Stahl-Hennig C, Hunsmann G (1992) Identification of a gag protein epitope conserved among all four groups of primate immunodeficiency viruses by using monoclonal antibodies. J Gen Virol 73:2721–2724

    Google Scholar 

  20. Otteken A, Voss G, Hunsmann G (1993) Mapping of the human immunodeficiency virus type 2 envelope glycoprotein CD4 binding region and fusion domain with truncated proteins expressed by recombinant vaccinia viruses. Virology 194:37–43

    Google Scholar 

  21. Baumgarten H, Schulze M (1985) Nachweise von antigenspezifischen Antikörpern. In: Peters JH, Baumgarten H, Schulze M (eds) Monoklonale Antikörper. Springer, Berlin Heidelberg New York Tokyo, pp 103–160

    Google Scholar 

  22. Putkonen P, Bottiger B, Warstedt K, Thorstensson R, Albert J, Biberfeld G (1989) Experimental infection of cynomolgus monkeys (Macaca fascicularis) with HIV-2. J AIDS 2:366–373

    Google Scholar 

  23. Schneider J, Hunsmann G (1978) Surface expression of murine leukemia virus structural polypeptides on host cells and the virion. Int J Cancer 22:204–213

    Google Scholar 

  24. Schneider J, Yamamoto N, Hinuma Y, Hunsmann G (1984) Sera from adult T-cell leukemia patients react with envelope and core polypeptides of adult T-cell leukemia virus. Virology 132:1–11

    Google Scholar 

  25. Schneider J, Lüke W, Kirchhoff F, Jentsch KD, Jung R, Jurkiewicz E, Stahl-Hennig C, Klemm E, Hunsmann G (1990) Isolation and characterisation of HIV-2ben, a new HIV-2 from a patient with predominantly neurological defects. AIDS 4:455–457

    Google Scholar 

  26. Speijers GJ, Danse LH, Benvery EC, Strik JJ, Vos JG (1988) Local reactions of the Saponin Quil A and a Quil A containing iscom measles vaccine after intramuscular injection of rats: a comparison with the effect of DPT-polio vaccine. Fundam Appl Toxicol 10:425–430

    Google Scholar 

  27. Stahl-Hennig C, Herchenröder O, Nick S, Evers M, Stille-Siegener M, Jentsch KD, Kirchhoff F, Tolle T, Gatesman TJ, Lüke W, Hunsmann G (1990) Experimental infection of macaques with HIV-2ben, a novel HIV-2 isolate. AIDS 4:611–617

    Google Scholar 

  28. Veronese FD, Copeland TD, Oroszlan S, Gallo RC, Sarngadharan MG (1988) Biochemical and immunological analysis of human immundeficiency virus gag gene products p17and p24. J Virology 62:795–801

    Google Scholar 

  29. Voss G, Kirchhoff F, Nick S, Moosmayer D, Gelderblom H, Hunsmann G (1992) Morphogenesis of recombinant HIV-2 gag core particles. Virus Res 24:197–210

    Google Scholar 

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Authors and Affiliations

  1. Deutsches Primatenzentrum, Abteilung Virologie und Immunologie, Kellnerweg 4, W-3400, Göttingen, Germany

    Arne-C. Faißt, Ahlert Otteken, Sigrid Nick & Gerhard Hunsmann

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  1. Arne-C. Faißt
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  2. Ahlert Otteken
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  3. Sigrid Nick
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  4. Gerhard Hunsmann
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Faißt, AC., Otteken, A., Nick, S. et al. Generation, characterization and cross-reactivities of monoclonal antibodies against the p24 core protein and the gp130 envelope glycoprotein of HIV-2ben . Med Microbiol Immunol 182, 119–128 (1993). https://doi.org/10.1007/BF00190264

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  • DOI: https://doi.org/10.1007/BF00190264

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