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The extravascular contractile system in the human placenta

Morphological and immunocytochemical investigations

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Abstract

In the human placenta, besides the fetal blood vessel system a second extravascular contractile system exists. It is localized in the chorionic plate and runs in a longitudinal direction and adjacent to fetal blood vessels into the stem villi, where it forms perivascular contractile sheaths. Characteristically, cells of the extravascular contractile system are extremely long and spindle-shaped and give rise to fine cell processes, by which they obviously contact each other or insert into the basement membrane of the trophoblast. They show immunoreactivity with desmin, vimentin, α-actin, myosin, nitric oxide synthase type I (brain form) and dipeptidyl peptidase IV. The ultrastructure suggests that cells of the extravascular contractile system are related to smooth muscle cells, including subpopulations with myofibroblastic features. In stem villi a few cells are nitric oxide synthase type I immunoreactive. These cells are thought to be specialized smooth-muscle-like cells of the extravascular contractile system or cells of the extravascular contractile system related to paraneurons that generate nitric oxide, which, in turn, may modulate the tone of perivascular contractile sheaths. The high dipeptidyl peptidase IV activity suggests that modulation of the extravascular contractile system may also occur by substance P.

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References

  • Babcock RJ (1969) Smooth muscle in the human placenta. Am J Obstet Gynecol 105:612–615

    Google Scholar 

  • Becker V, Schiebler T, Kubli F (1981) Die Plazenta des Menschen. Thieme, Stuttgart, p 55

    Google Scholar 

  • Beham A, Denk H, Desoye G (1988) The distribution of intermediate filament proteins, actin and desmoplakins in human placental tissue as revealed by polyclonal and monoclonal antibodies. Placenta 9:479–492

    Google Scholar 

  • Bertolini R, Reißig D, Schippel K (1969) Elektronenmikroskopische Befunde an den Zellen in der Chorionplatte der reifen menschlichen Plazenta. Z Mikrosk-Anat Forsch 80:358–368

    Google Scholar 

  • Boura ALA, Walters WAW (1991) Autacoids and the control of vascular tone in the human umbilical-placental circulation. Placenta 12:453–477

    Google Scholar 

  • Demir R, Demir N, Kohnen G, Kosanke G, Mironov V, Üstünel I (1992) Ultrastructure and distribution of myofibroblast-like cells in human placental stem villi. Electron Microsc 3:509–510

    Google Scholar 

  • Dubreuil G, Riviére M (1932) Formations fibro-musculaires du chorion et des villosités du placenta humain. CR Seances Soc Biol Fil 111:170–172

    Google Scholar 

  • Feller AC, Schneider H, Schmidt D, Parwaresch MR (1985) Myofibroblasts as a major cellular constituent of villous stroma in human placenta. Placenta 6:405–415

    Google Scholar 

  • Gown AM (1990) The mysteries of the myofibroblast (partially) unmasked. Lab Invest 63:1–3

    Google Scholar 

  • Graf R, Frank HG, Öney T (1992) Histochemical and immunocytochemical investigations of the fetal extravascular and vascular contractile system in the normal placenta and during preeclampsia. In: Neubert D, Kavlock RJ, Merker HJ, Klein J (eds) Risk assessment of prenatally-induced adverse health effects. Springer, Berlin Heidelberg New York, pp 537–550

    Google Scholar 

  • Graf R, Langer J-U, Schönfelder G, Hartel-Schenk S, Reutter W (1993) The extravascular system of the human placenta. Placenta 14:A.25

    Google Scholar 

  • Hagemann A, Nielsen AH, Poulsen K (1994) The uteroplacental renin-angiotensin system: a review. Exp Clin Endocrinol (in press)

  • Happe H (1906) Beobachtungen an Eihäuten junger menschlicher Eier. Anat Hefte 32:173–212

    Google Scholar 

  • Hartel S, Hanski C, Kreisel W, Hoffmann C, Mauck J, Reutter W (1987) Rapid purification of dipeptidyl peptidase IV from rat liver plasma membranes. Biochim Biophys Acta 924:543–547

    Google Scholar 

  • Heymann E, Mentlein R (1984) Beeinflußt Dipeptidylpeptidase IV Blutdruck und Gerinnung? Klin Wochenschr 62:2–10

    Google Scholar 

  • Hsi BL, Yeh CJG (1988) Monoclonal antibodies to placental vascular structures. Trophoblast Res 3:139–148

    Google Scholar 

  • Huszar G, Bailey P (1979a) Isolation and characterization of myosin in the human term placenta. Am J Obstet Gynecol 135:707–712

    Google Scholar 

  • Huszar G, Bailey P (1979b) Relationship between actin-myosin interaction and myosin light chain phosphorylation in human placental smooth muscle. Am J Obstet Gynecol 135:718–726

    Google Scholar 

  • Kaufmann P, Sen DK, Schweikhart G (1979) Classification of human placental villi. I. Histology. Cell Tissue Res 200:409–423

    Google Scholar 

  • King TM, Gröschel-Stewart U (1965) Placental contractile protein. Am J Obstet Gynecol 93:253–258

    Google Scholar 

  • Kloos K, Vogel M (1974) Pathologie der Perinatalperiode. Thieme, Stuttgart, pp 22–27

    Google Scholar 

  • Kohnen G, Mironov V, Demir R, Castellucci M, Kaufmann P (1993) Immunhistochemie und Ultrastruktur kontraktiler Zellen im Zottenstroma der menschlichen Placenta. Ann Anat 175 [Suppl]:37

    Google Scholar 

  • Krantz KE, Parker JC (1963) Contractile properties of the smooth muscle in the human placenta. Clin Obstet Gynecol 6:26–38

    Google Scholar 

  • Lojda Z, Gossrau R, Stoward PJ (1991) Proteases. In: Stoward PJ, Pearse AGE (eds) Histochemistry. Theoretical and applied, vol 3. Enzyme histochemistry, 4th edn. Churchill Livingstone, Edinburgh, pp 305–309

    Google Scholar 

  • McDonald JK, Barrett AJ (1986) Mammalian proteases: a glossary and bibliography, vol 2. Academic Press, London

    Google Scholar 

  • Michael C (1974) Actomyosin content of the human placenta. J Obstet Gynaecol 81:307–310

    CAS  PubMed  Google Scholar 

  • Moncada S, Palmer RMJ, Higgs EA (1991) Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol Rev 43:109–142

    CAS  PubMed  Google Scholar 

  • Myatt L (1992) Control of vascular resistance in the human placenta. Placenta 13:329–341

    Google Scholar 

  • Pappas PW (1971) The use of a chrome alum-gelatin (subbing) solution as a general adhesive for paraffin sections. Stain Technol 46:121–124

    Google Scholar 

  • Püschel G, Mentlein R, Heymann E (1982) Isolation and characterization of dipeptidyl peptidase IV from human placenta. Eur J Biochem 126:359–365

    Google Scholar 

  • Romeis B (1968) Mikroskopische Technik. Oldenbourg, Munich

    Google Scholar 

  • Schmidt HHHW, Böhme E (1990) NO, ein hormonaler Wirkstoff. Med Mol Pharmacol 3:74–81

    Google Scholar 

  • Schmidt HHHW, Gagne GD, Nakane M, Pollock JS, Miller MF, Murad F (1992) Mapping of neural nitric oxide synthase in the rat suggests frequent co-localization with NADPH-diaphorase but not with soluble guanylyl cyclase, and novel paraneuronal functions for nitrinergic signal transduction. J Histochem Cytochem 40:1439–1456

    Google Scholar 

  • Schönfelder G, Graf R, Schmidt HHHW (1993) A possible regulation of the extravascular contractile system in human placenta by nitric oxide synthase immunoreactive cells. Placenta 14:A.69

    Google Scholar 

  • Spanner R (1936) Mütterlicher und kindlicher Kreislauf der menschlichen Plazenta und seine Strombahnen. Z Anat Entwicklungsgesch 105:163–242

    Google Scholar 

  • Vogel M (1992) Atlas der morphologischen Plazentadiagnostik. Springer, Berlin Heidelberg New York, pp 4 and 80–91

    Google Scholar 

Download references

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Authors and Affiliations

  1. Institut für Anatomie, Freie Universität Berlin, Königin-Luise-Strasse 15, D-14195, Berlin, Germany

    R. Graf, J.-U. Langer & G. Schönfelder

  2. Frauenklinik und Poliklinik, UK Steglitz, Freie Universität Berlin, Hindenburgdamm 30, D-12203, Berlin, Germany

    T. Öney

  3. Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-14195, Berlin, Germany

    S. Hartel-Schenk & W. Reutter

  4. Institut für Biochemie und Pathobiochemie, Medizinische Universität Würzburg, Versbacher Strasse 5, D-97078, Würzburg, Germany

    H. H. H. W. Schmidt

Authors
  1. R. Graf
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  2. J.-U. Langer
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  3. G. Schönfelder
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  4. T. Öney
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  5. S. Hartel-Schenk
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  6. W. Reutter
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  7. H. H. H. W. Schmidt
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Graf, R., Langer, JU., Schönfelder, G. et al. The extravascular contractile system in the human placenta. Anat Embryol 190, 541–548 (1994). https://doi.org/10.1007/BF00190104

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  • DOI: https://doi.org/10.1007/BF00190104

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