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Pneumococcal C and type polysaccharide detection in the concentrated urine of patients with bacteremia

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Abstract

The C polysaccharide of Streptococcus pneumoniae was detected in the concentrated urine of 23 of 33 patients with pneumococcal bacteremia using latex agglutination. Type-specific polysaccharides were detected in the urine of 17 of these 33 patients including 4 patients lacking C polysaccharide in their urine. These 4 with the 23 detected above gave a total sensitivity of 82% (27/33). The concentrated urine from an additional 11 patients with other bacteremias were tested by C polysaccharide and type-specific reagents and were negative. C polysaccharide detection in the concentrated urine of patients may be helpful in the diagnosis of pneumococcal infections.

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References

  1. Anhalt JP, Kenny GE, Rytel MW (1978) Detection of microbial antigens by counterimmu-nopelectrophoresis. American Society for Microbiology, Cumulative Techniques in Clinical Microbiology #8, Washington, pp 32–8

  2. Barrett-Connor E (1971) The nonvalue of sputum culture in the diagnosis of pneumococcal pneumonia. Am Rev Respir Dis 103:845–848

    Google Scholar 

  3. Bolan G, Ajello GW, Hayes PS, Lehmann D, Feeley JC, Perlino C, Broome CV (1986) The utility of commercial latex agglutination tests for detection of H. influenzas type b and S. pneumoniae antigens in bacteremic pneumonia patients. Sept. 28, 1986, New Orleans, Louisiana, abstract no. 73

  4. Coonrod JD (1983) Urine as an antigen reservoir for diagnosis of infectious diseases. Am J Med 75:85–92

    Google Scholar 

  5. Doskeland SO, Berdal BP (1980) Bacterial antigen detection in body fluid: methods for rapid antigen concentration and reduction of nonspecific reactions. J Clin Microbiol 11:380–384

    Google Scholar 

  6. Kalin M, Lindberg AA (1983) Diagnosis of pneumococcal pneumonia: a comparison between microscopic examination of expectorate, antigen detection and cultural procedures. Scand J Infect Dis 15:247–255

    Google Scholar 

  7. Kenny GE (1983) Counterimmunoelectrophoresis for the diagnosis of pneumococcal respiratory and other infections. In: Coonrod JD, Kunz L, Ferraro MJ (eds) The direct detection of microorganisms in clinical samples. Academic press, New York, pp 105–111

    Google Scholar 

  8. Kronvall G (1973) A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci. J Med Microbiol 6:187–190

    Google Scholar 

  9. Krook A, Holmberg H, Sjogren AM (1987) A new coagglutination test for detection Pneumococcal C polysaccharide. Eur J Clin Microbiol 6:68–69

    Google Scholar 

  10. Mayer ME, Geiseler PJ, Harris B (1983) Coagglutination for detection and serotyping of bacterial antigens: usefulness in acute pneumonias. Diagn Microbiol Infect Dis 1:277–285

    Google Scholar 

  11. O'Neill KP, Lloyd-Evans N, Campbell H, Forgie IM, Sabbally S, Greenwood BM (1989) Latex agglutination test for diagnosing pneumococcal pneumonia in children in developing countries. Br Med J 298:1061–1064

    Google Scholar 

  12. Ramsey BW, Marcuse EK, Foy HM, Cooney MK, Allen I, Brewer D, Smith AL (1986) Use of bacterial antigen detection in the diagnosis of pediatric respiratory tract infection. Pediatrics 78:1–9

    Google Scholar 

  13. Steinhoff MC, Simoes E, John TJ, Jadhav M, Pereira SM (1986) Antigen detection in pneumonia: A diagnostic and epidemiologic tool for developing countries (abstract). Pediatr Res 472:20, part 2:233 A

    Google Scholar 

  14. Thirumoorthi NC, Dajani AS (1979) Comparison of staphylococcal coagglutination, latex agglutination and counterimmunoelectrophoresis for bacterial antigen detection. J Clin Microbiol 9:28–32

    Google Scholar 

  15. Tugwell P, Greenwood BM (1975) Pneumococcal antigen in lobar pneumonia. J Clin Pathol 28:118–123

    Google Scholar 

  16. Turner RB, Hayden FG, Hendley JO (1983) Counterimmunoelectrophoresis of urine for diagnosis of bacterial pneumonia in pediatric outpatients. Pediatrics 71:780–783

    Google Scholar 

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Authors and Affiliations

  1. Departments of Pediatrics, Medicine, and Pathology, State University of New York, Health Sciences Center at Brooklyn/Kings County Hospital Center, 451 Clarkson Avenue, 11203, NY, USA

    Kenneth Bromberg, Gaylene Tannis & Alma Rodgers

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  1. Kenneth Bromberg
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  2. Gaylene Tannis
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  3. Alma Rodgers
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Bromberg, K., Tannis, G. & Rodgers, A. Pneumococcal C and type polysaccharide detection in the concentrated urine of patients with bacteremia. Med Microbiol Immunol 179, 335–338 (1990). https://doi.org/10.1007/BF00189611

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  • DOI: https://doi.org/10.1007/BF00189611

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