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Nuclear DNA analysis and prognosis in carcinoma of the thyroid gland

A nationwide study in iceland on carcinomas diagnosed 1955–1990

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Abstract

The purpose of this study was to ascertain whether DNA ploidy status and S-phase fraction affected the prognosis of patients with carcinoma of the thyroid gland. We reviewed all malignant thyroid tumours diagnosed in Iceland from 1955 to 1990. In all, 494 thyroid carcinomas were diagnosed during that period. By analysing tumour material from paraffin blocks by flow cytometry we were able to evaluate the ploidy status in 424 tumours and the S-phase value in 417 tumours. We detected aneuploid cell populations in 9.7% of papillary carcinomas, 24.3% of follicular carcinomas, 42.9% of medullary carcinomas and 78.6% of anaplastic carcinomas. Some 57% of tumours, mainly papillary carcinomas, had an S-phase value of less then 3%, whereas most of the other histological types of carcinoma, including all the anaplastic tumours, had an S-phase value of ≥3%. Univariate analysis indicated that both ploidy status and S-phase fraction were significant variables. When taking into account known prognostic variables of thyroid carcinoma in a multivariate analysis, however, neither ploidy status nor S-phase value proved significant. We conclude that DNA ploidy status and S-phase values are not independent prognostic factors in thyroid carcinoma.

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References

  1. Bäckdahl M, Tallroth E, Auer G, Forsslund G, Granberg PO, Lundell G, Löwhagen T (1985) Prognostic value of nuclear DNA content in medullary thyroid carcinoma. World J Surg 9:980–987

    Google Scholar 

  2. Bäckdahl M, Auer G, Forsslund G, Granberg PO, Lundell G, Löwhagen T, Zetterberg A (1986) Prognostic value of nuclear DNA content in follicular thyroid tumours. Acta Chir Scand 152:1–7

    Google Scholar 

  3. Bengtsson A, Malmaeus J, Grimelius L, Johanson H, Pontén J, Rastad J, Åkerström G (1984) Measurement of nuclear DNA content in thyroid diagnosis. World J Surg 8:481–486

    Google Scholar 

  4. Bergholm U, Adami HO, Auer G, Bergström R, Bäckdahl M, Grimelius L, Hansson G, Ljungberg O, Wilander E, The Swedish Study Group (1989) Histopathologic characteristics and nuclear DNA content as a prognostic factors in medullary thyroid carcinoma. A nationwide study in Sweden. Cancer 64:135–142

    Google Scholar 

  5. Bondeson L, Azavedo E, Bondeson AG, Caspersson T, Ljungberg O (1986) Nuclear DNA content and behavior of oxyphil thyroid tumours. Cancer 58:672–675

    Google Scholar 

  6. Britton LC, Wilson TO, Gaffey TA, Cha SS, Samuel Wiand H, Podratz KC (1990) DNA ploidy in endometrial carcinoma: major objective factor. Mayo Clin Proc 65:643

    Google Scholar 

  7. Bronner MP, Clevenger CV, Edmonds PR, Lowel DM, McFarland MM, LiVolsi VA (1988) Flow cytometric analysis of DNA content in Hürthle cell adenomas and carcinomas of the thyroid. Am J Clin Pathol 89:764–769

    Google Scholar 

  8. Camargo RS, Scafuri AG, Castro de Tolosa EM, Ferreira EAB (1992) DNA image cytometric analysis of differentiated thyroid adenocarcinoma specimens. Am J Surg 164:640–645

    Google Scholar 

  9. Christov K (1986) Flow cytometric DNA measurements in human thyroid tumors. Virchows Arch B 51:255–263

    Google Scholar 

  10. Cohn K, Bäckdahl M, Forsslund G, Auer JG, Lundell G, Löwhagen T, Tallroth E, Willems JS, Zetterberg A, Granberg PO (1984) Prognostic value of nuclear DNA content in papillary thyroid carcinoma. World J Surg 8:474–480

    Google Scholar 

  11. Cox DR (1972) Regression models and life tables. J R Stat Soc 34:187–220

    Google Scholar 

  12. EGRET Reference Manual (1990) Statistics and epidemiology research corporation. SERC, Seattle

    Google Scholar 

  13. El-Naggar AK, Batsakis JG, Luna MA, Hickey RC (1988) Hürthle cell tumors of the thyroid. A flow cytometric DNA analysis. Arch Otolaryngol Head Neck Surg 114:520–521

    Google Scholar 

  14. Engeland A. Haldorsen T, Tretli S, Hakulinen T, Hörte LG, Luostarinen T, Magnus K, Schou G, Sigvaldason H. Strom HH, Tulinius H, Vaittinen P (1993) Prediction of cancer incidence in the Nordic countries up to the years 2000 and 2010. A collaborative study of the five Nordic cancer registries. APMIS [Suppl 38]: 101:1–124

    Google Scholar 

  15. Fukunaga M, Shinozaki N, Endo Y, Ushigome S (1992) Atypical adenoma of the thyroid. A clinicopathologic and flow cytometric DNA study in comparison with other follicular neoplasms. Acta Pathol Jpn 42:632–638

    Google Scholar 

  16. Grant CS, Hay ID, Ryan JJ, Bergstralh EJ, Rainwater LM, Goellner JR (1990) Diagnostic and prognostic utility of flow cytometric DNA measurements in follicular thyroid tumours. World J Surg 14:283–290

    Google Scholar 

  17. Hamming JF, Schelfhout LJDM, Cornelisse CJ, van de Velde CJH, Goslings BM, Hermans J, Fleuren GJ (1988) Prognostic value of nuclear DNA content in papillary and follicular thyroid cancer. World J Surg 12:503–508

    Google Scholar 

  18. Hedinger C, Williams ED, Sobin LH (1988) Histologic typing of thyroid tumours, 2nd edn. (International Histological Classification of Tumours, 11) World Health Organisation. Springer, Berlin Heidelberg New York

    Google Scholar 

  19. Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA (1983) Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem 31:1333–1335

    Google Scholar 

  20. Hiddeman W, Schumann J, Andreeff M, Barlogie B, Herman CJ, Leif RC, Mayall BH, Murphy RF, Sandberg AA (1984) Convention on nomenclature for DNA cytometry. Cytometry 5:445–446

    Google Scholar 

  21. Hostetter AL, Hrafnkelsson J, Wingren SOW, Eneström S, Nordenskjöld B (1988) A comparative study of DNA cytometry methods for benign and malignant thyroid tissue. Am J Clin Pathol 89:760–763

    Google Scholar 

  22. Hruban RH, Huvos AG, Traganos F, Reuter V, Lieberman PH, Melamed MR (1990) Follicular neoplasms of the thyroid in men older than 50 years of age. A DNA flow cytometric study. Am J Clin Pathol 94:527–532

    Google Scholar 

  23. Joensuu H, Klemi P, Eerola E, Tuominen J (1986) Influence of cellular DNA content on survival in differentiated thyroid cancer. Cancer 58:2462–2467

    Google Scholar 

  24. Johannessen JV, Sobrinho-Simões M, Tangen KO, Lindmo T (1981) A flow cytometric deoxyribonucleic acid analysis of papillary thyroid carcinoma. Lab Invest 45:336–341

    Google Scholar 

  25. Johannessen JV, Sobrinho-Simões M, Lindmo T, Tangen KO (1982) The diagnostic value of flow cytometric DNA measurements in selected disorders of the human thyroid. Am J Clin Pathol 77:20–25

    Google Scholar 

  26. Jonasson JG, Hrafnkelsson J, Björnsson J (1989) Tumours in Iceland. 11. Malignant tumours of the thyroid gland. APMIS 97:625–630

    Google Scholar 

  27. Kallioniemi OP, Punnonen R, Mattila J, Lettinen M, Koivula T (1988) Prognostic significance of DNA index, multiploidy and S-phase fraction in ovarian cancer. Cancer 61:334–339

    Google Scholar 

  28. Kaplan EL, Meier P (1958) Non-parametric estimation from incomplete observations. J Am Stat Assoc 53:457–481

    Google Scholar 

  29. Klemi PJ, Joensuu H, Eerola E (1988) DNA aneuploidy in anaplastic carcinoma of the thyroid gland. Am J Clin Pathol 89:154–159

    Google Scholar 

  30. Lukes AS, Kohler MF, Pieper CF, Bentley R, Rodriguez GC, Soper JT, Clarke-Pearson DL, Bast RC, Berchuck A (1994) Multivariable analysis of DNA ploidy, p53, and HER-2/neu as prognostic factors in endometrial cancer. Cancer 73:2380–2385

    Google Scholar 

  31. Mizukami Y, Nonomura A, Michigishi T, Kosaka T, Noguchi M, Nakamura S, Hashimoto T (1992) Flow cytometric DNA measurement in benign and malignant human thyroid tissues. Anticancer Res 12:2213–2218

    Google Scholar 

  32. Oyama T, Vickery AL, Preffer FI, Colvin RB (1994) A comparative study of flow cytometry and histopathologic findings in thyroid follicular carcinomas and adenomas. Hum Pathol 25:271–275

    Google Scholar 

  33. Pasieka JL, Zedenius J, Auer G, Grimelius L, Höög A, Lundell G, Wallin G, Backdhal M (1992) Addition of nuclear DNA content to the AMES risk-group classification for papillary thyroid cancer. Surgery 112:1154–1160

    Google Scholar 

  34. Pyke CM, Hay ID, Goellner JR, Bergstralh EJ, van Heerden JA, Grant CS (1991) Prognostic significance of calcitonin immunoreactivity, amyloid staining, and flow cytometric DNA measurements in medullary thyroid carcinoma. Surgery 110:964–971

    Google Scholar 

  35. Schark C, Fulton N, Yashiro T, Stanislav G, Jacoby R, Strauss FH, Dytch H, Bibbo M, Kaplan EL (1992) The value of measurements of ras oncogenes and nuclear DNA analysis in the diagnosis of Hürthle cell tumors of the thyroid. World J Surg 16:745–752

    Google Scholar 

  36. Schelfhout LJDM, Cornelisse CJ, Goslings BM, Hamming JF, Kuipers-Dukshoorn NJ, van de Velde CJH, Fleuren GJ (1990) Frequency and degree of aneuploidy in benign and malignant thyroid neoplasms. Int J Cancer 45:16–20

    Google Scholar 

  37. Sierk AE, Askin FB, Reddick RL (1990) Pediatric thyroid cancer. Pediatr Pathol 10:877–893

    Google Scholar 

  38. Sigurdsson H, Baldedorp B, Borg Å, Dalberg M, Fernö M, Killander D, Olsson H (1990) Indicators of prognosis in node negative breast cancer. N Engl J Med 322:1045–1053

    Google Scholar 

  39. Soares J, Fonseca I, Limbert E, Falkmer UG, Falkmer S (1991) Prognostic implications of image cytometric assessments of nuclear DNA distribution pattern of neoplastic cells in thyroid medullary carcinoma. APMIS 99:745–754

    Google Scholar 

  40. St»l O, Klintenberg C, Franzén G, Risenberg B, Arvidsson S, Bjelkenkrantz K, Skoog L, Nordenskjöld B (1986) A comparison of static fluorometry and flow cytometry for the estimation of ploidy and DNA replication in human breast cancer. Breast Cancer Res Treat 7:15–22

    Google Scholar 

  41. Tallroth-Ekman E, Bäckdahl M, Löwhagen T, Auer G (1989) Nuclear DNA measurements on thyroid carcinoma in young patients. Acta Oncol 28:475–479

    Google Scholar 

  42. Tallroth-Ekman E, Wallin G, Bäckdahl M, Löwhagen T, Auer G (1989) Nuclear DNA content in anaplastic giant-cell thyroid carcinoma. Am J Clin Oncol (CCT) 12:442–446

    Google Scholar 

  43. Tallroth-Ekman E, Bergholm U, Bäckdahl M, Adami HO, Bergström R, Grimelius L, Auer G, The Swedish Medullary Thyroid Cancer Study Group (1990) Nuclear DNA content and survival in medullary thyroid carcinoma. Cancer 65:511–517

    Google Scholar 

  44. Thornthwaite JT, Sugarbaker EV, Temple WJ (1980) Preparation of tissues for DNA flow cytometric analysis. Cytometry 1:229–237

    Google Scholar 

  45. Tsuchiya A, Sekikawa K, Ando Y, Suzuki S, Kimijima I, Abe R (1990) Flow cytometric DNA analysis of thyroid carcinoma. Jpn J Surg 20:410–514

    Google Scholar 

  46. Zedenius J, Auer G, Bäckdahl M, Falkmer U, Grimelius L, Lundell G, Wallin G (1992) Follicular tumors of the thyroid gland: diagnosis, clinical aspects and nuclear DNA analysis. World J Surg 16:589–594

    Google Scholar 

  47. Zimmerman D, Hay I, Gough IR, Goellner JR, Ryan JJ, Grant CS, McGonahey WM (1988) Papillary thyroid carcinoma in children and adults: long-term follow-up of 1039 patients conservatively treated at one institution during three decades. Surgery 104:1157–1166

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, University of Iceland, P.O. Box 1465, 121, Reykjavík, Iceland

    J. G. Jónasson

  2. Department of Oncology, University Hospital, and Icelandic Cancer Registry, Reykjavík, Iceland

    J. Hrafnkelsson

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  1. J. G. Jónasson
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Jónasson, J.G., Hrafnkelsson, J. Nuclear DNA analysis and prognosis in carcinoma of the thyroid gland. Vichows Archiv A Pathol Anat 425, 349–355 (1994). https://doi.org/10.1007/BF00189572

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  • DOI: https://doi.org/10.1007/BF00189572

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