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Elicitation of Taxus sp. cell cultures for production of taxol

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Summary

The addition of cell extracts and cultures filtrate of Pencillium minioluteum, Botrytis cinerea, Verticillium dahliae, and Gilocladium deliqucescens on the tenth day after transferring Taxus sp. (RO1-M28) cell suspensions into an induction medium, further improved the production of TaxolFootnote 1 and total taxanes. Arachidonic acid (1mg/L) addition at the time of inoculation increased Taxol production by 150%. Oxidative stress induction and copper sulphate or sodium orthovanadate addition had no effect on Taxol production. Three categories of elicitors; those specifically stimulating Taxol production, those specifically stimulating the producion of other taxanes, and those stimulating taxane production uniformly, could be identified. The biosynthetic site of action of these elicitors is currently not known.

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Notes

  1. Taxol is a registered trademark of Bristol Myers Squibb for paclitaxel.

References

  • Castoria, R., Altamura, M.M., Fabbri, A.A., Tomassi, M., and Fanelli, C. (1995). Plant Physiol. 145, 209–214.

    Google Scholar 

  • Choi, D., Bostock, R.M., Avdiushko, S., and Hilderbrand, D.F. (1994). Proc. Natl. Acad. Sci. USA. 91, 2329–2333.

    Google Scholar 

  • Christen, A.A., Gibson, D.M., and Bland, T. (1991). U.S. Patent 5019504.

  • Cline, S.D., McHale, R.J., and Coscia, C.J. (1993). J. Nat. Products 56(8), 1219–1228.

    Google Scholar 

  • Cragg, G.M., Schepartz, S.A., Suffness, M., and Grever, M.R. (1993). J. Nat. Products 56(10), 1657–1668.

    Google Scholar 

  • Eilert, U., and Constabel, F. (1986). J. Plant Physiol. 125, 167–172.

    Google Scholar 

  • Fetto-neto, A.G., Dicosmo, F., Reynolds, W.F., and Sataka, K. (1992). Bio/Technology 10, 1572–1575.

    Google Scholar 

  • Fetto-neto, A.G., Melanson, S.J., Nicholson, S.A., Pennington, J.J., and Dicosmo, F., (1994). Biotechnology and Bioengineering 44, 967–971.

    Google Scholar 

  • Ketchum, R.E.B., and Gibson, D.M. (1993). J. Liq. Chromatography 16(12), 2519–2530.

    Google Scholar 

  • Payne, G.F., Bringi, V., Prince, C.L., and Shuler, M.L. Plant Cell and Tissue Culture in Liquid Systems, Hanser Publishers, Munich, 1992.

    Google Scholar 

  • Srinivasan, V., Pestchanker, L., Moser, S., Hirasuna, T.J., Taticek, R.A., Shuler, M.L. (1995). Biotechnlogy and Bioengineering. In press.

  • Srinivasan, V., Moser, S., and Shuler, M.L. (1995). J. Nat. Products. Communicated.

  • Strobel, G., Stierle, A., van Kujik, F. (1992). Plant Science 84, 65–74.

    Google Scholar 

  • Suvamalatha, G., Rajendran, L., Ravishankar, G.A., and Venkatraman, L.V. (1994). Biotechnology Letters 16(12), 1275–1280.

    Google Scholar 

  • Witherup, K.M., Look, S.A., Stasko, W.M., McGloud, O.G., Issaq, H.J., and Muschik, G.M. (1989). J.Liq.Chromatography 12, 2117–2132.

    Google Scholar 

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Ciddi, V., Srinivasan, V. & Shuler, M.L. Elicitation of Taxus sp. cell cultures for production of taxol. Biotechnol Lett 17, 1343–1346 (1995). https://doi.org/10.1007/BF00189223

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  • DOI: https://doi.org/10.1007/BF00189223

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