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Immunogenetics

, Volume 40, Issue 2, pp 83–99 | Cite as

Immunoglobulin light chain class multiplicity and alternative organizational forms in early vertebrate phylogeny

  • Jonathan P. Rast
  • Michele K. Anderson
  • Ronda T. Litman
  • M. Margittai
  • Gary W. Litman
  • Tatsuya Ota
  • Michael J. Shamblott
Original Paper

Abstract

The prototypic chondrichthyan immunoglobulin (Ig) light chain type (type I) isolated from Heterodontus francisci (horned shark) has a clustered organization in which variable (V), joining (J), and constant (C) elements are in relatively close linkage (V-J-C). Using a polymerase chain reaction-based approach on a light chain peptide sequence from the holocephalan, Hydrolagus colliei (spotted ratfish), it was possible to isolate members of a second light chain gene family. A probe to this light chain (type II) detects homologs in two orders of elasmobranchs, Heterodontus, a galeomorph and Raja erinacea (little skate), a batoid, suggesting that this light chain type may be present throughout the cartilaginous fishes. In all cases, V, J, and C regions of the type II gene are arranged in closely linked clusters typical of all known Ig genes in cartilaginous fishes. All representatives of this type II gene family are joined in the germline. A third (kappa-like) light chain type from Heterodontus is described. These findings establish that a degree of light chain class complexity comparable to that of the mammals is present in the most phylogenetically distant extant jawed vertebrates and that the phenomenon of germline-joined (pre-rearranged) genes, described originally in the heavy chain genes of cartilaginous fishes, extends to light chain genes.

Keywords

Light Chain Class Complexity Chain Gene Chain Peptide Heavy Chain Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Jonathan P. Rast
    • 1
  • Michele K. Anderson
    • 1
  • Ronda T. Litman
    • 1
  • M. Margittai
    • 1
  • Gary W. Litman
    • 1
  • Tatsuya Ota
    • 2
  • Michael J. Shamblott
    • 3
  1. 1.All Children's HospitalUniversity of South FloridaSt. PetersburgUSA
  2. 2.Institute of Molecular Evolutionary GeneticsThe Pennsylvania State UniversityUniversity ParkUSA
  3. 3.Center of Marine BiotechnologyUniversity of MarylandBaltimoreUSA

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