Abstract
In previous studies, aluminium was found to retard bacterial growth and enhance porphyrin formation in Arthrobacter aurescens RS-2. The aim of this study was to establish the mechanism of action of aluminium which leads to increased porphyrin production. Cultures of Arthrobacter aurescens RS-2 were incubated in the absence and presence of 0.74 mm aluminium. After 6 and 24 h of incubation, various parameters of the haem biosynthetic pathway were determined. After 6 h of incubation with aluminium, the activities of the enzymes aminolevulinate synthase (ALAS), aminolevulinate dehydratase (ALAD), porphobilinogen deaminase (PBGD) and uroporphyrinogen decarboxylase (UROD) were increased by 120, 170, 190 and 203%, respectively, while that of ferrochelatase (FC) was found to be unchanged. However, after 24 h of incubation, no change in the activities of ALAS and ALAD was noted, while an about 2-fold increase in PBGD and UROD activities were observed. FC activity was decreased by 63%. It was concluded that aluminium exerts its effect by inducing the enzymes PBGD and UROD rather than by a direct or indirect effect on ALAS. Its effect on the final step in the haem biosynthetic pathway is discussed.
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References
Abdulla M, Svensson S, Haeger-Aronson B. 1979 Antagonistic effect of zinc and aluminum on lead inhibition of delta amino-levulinic- acid dehydratase EC-4.2.1.24. Arch Environ Health 34, 464–469.
Avissar YJ, Nadler KD 1978 Stimulation of tetrapyrrole formation in Rhizobium japonicum by restricted aeration. J Bacteriol 135, 782–789.
Berko G, Durko I. 1972 A new possibility for the demonstration of amino-laevulinic acid in urine on the basis of Mauzerall-Granick method. Clin Chim Acta 37, 443–447.
Bia MJ, Cooper K, Schall S, et al. 1989 Aluminum induced anemia: pathogenesis and treatment in patients on chronic hemodialysis. Kidney Int 36, 852–858.
Brooker JD, Srivastava G, May BK, Elliott WH. 1982 Radiochemical assay for δ-aminolevulinate synthase. Enzyme 28, 109–119.
Buchet JP, Louwerys R, Hassoun A, et al. 1987 Effect of aluminum on porphyrin metabolism in hemodialyzed patients. Nephron 46, 360–363.
Buttery JE, Stuart S. 1991 Measurement of porphobilinogen in urine by a simple resin method with use of a surrogate standard. Clin Chem 37, 2133–2136.
Chmielnicka J, Nasiadek M, Lewandowska-Zyndul E. 1994 The effect of aluminum chloride on some steps of heme biosynthesis in rats after oral exposure. Biol Trace Element Res 40, 127–136.
Del C Battle AM, Ferramola AM, Grinstein M. 1967 Purification and general properties of delta-amino-levulinate dehydratase from cow liver. Biochem J 104, 244–249.
Deybach JC, de Verneuil H, Nordmann Y. 1981 The inherited enzymatic defect in porphyria variegata. Hum Genet 58, 425–428.
Fontanellas A, Coronel F, Santos JL, et al. 1994 Heme biosynthesis in uremic patients on CAPD or hemodialysis. Kidney Int 45, 220–223.
Javor GT, Febre EF. 1992 Enzymatic basis of thiol-stimulated secretion of porphyrins by Escherichia coli. J Bacteriol 174, 1072–1075.
Lascelles J. 1968 The regulation of haem and chlorophyll synthesis. Biochem Soc Symp 28, 49–59.
Lim CK, Peters TJ. 1984 Urine and faecal porphyrin profiles by reverse-phase high performance liquid chromatography in the porphyrias. Clin Chem Acta 139, 55–65.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJH. 1951 Protein measurement with folin-phenol reagent. J Biol Chem 193, 265- 275.
Luo G, Lim CK. 1991 Random decarboxylation of uroporphyrinogen III by human hepatic uroporphyrinogen decarboxylase. J Chromatogr 566, 409–413.
Macdonald TL, Martin RB. 1988 Aluminium ion in biological systems. Trend Biochem Sci 13, 15–19.
Magnussen CR, Levine JB, Doherty JM, Cheesman JO, Tschudy DP. 1974 A red cell enzyme method for diagnosis of acute intermittent porphyria. Blood 44, 857–868.
May BK, Bawden MJ. 1989 Control of heme biosynthesis in animals. Sem Hematol 26, 150–156.
McGonigle RJ, Parsons V. 1985 Aluminium-induced anaemia in haemodialysis patients. Nephron 39, 1–9.
McManus J, Blake D, Ratnaike S. 1988 An assay of uroporphyrinogen decarboxylase in erythrocytes. Clin Chem 34, 2355–2357.
Oelze J. 1992 Light and oxygen regulation of the synthesis of bacteriochlorophylls a and c in Chloroflexus aurantiacus. J Bacteriol 174, 5021–5026.
Philipp-Dormston WK, Doss M. 1973 Comparison of porphyrin and heme biosynthesis in various heterotrophic bacteria. Enzyme 16, 57–64.
Rosenlof K, Fyhrquist F, Tenhunen R. 1990 Erythropoietin, aluminium, and anaemia in patients on haemodialysis. Lancet 335, 247–249.
Rosseland BO, Eldhuset TD, Staurnes M. 1990 Environmental effect of aluminum. Environ Geochem Health 12, 17–27.
Scharf R, Zimmels Y, Kimchie S. 1993 Metal induced extracellular protein excretion in Arthrobacter aurescens. FEMS Microbiol Lett 109, 139–144.
Scharf R, Mamet R, Zimmels Y, Kimchie S, Schoenfeld, N. 1994 Evidence for the interference of aluminum with bacterial porphyrin biosynthesis. Bio Metals 7, 135–141.
Schoenfeld N, Mamet R. 1991 High-performance liquid chromatographic detection of pitfalls in porphobilinogen deaminase determination J Chromatogr Biomed Appl 570, 51–64.
Tait GH. 1973 Control of aminolaevulinate synthase in Micrococcus denitrificans. Enzyme 16, 21–27.
Witters HE, Van Puymbroeck S, Van Den Sande I, Vanderborght OLJ. 1990 Haematological disturbances and osmotic shifts in rainbow trout, Onchorhynchus mykiss [Walbaum] under acid and aluminum exposure. J Comp Physiol B 160, 563–571.
Wood M, Cooper JE. 1988 Acidity, aluminum and multiplication of Rhizobium trifolii: possible mechanisms of aluminum toxicity. Soil Biol Biochem 20, 95–99.
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Mamet, R., Scharf, R., Zimmels, Y. et al. Mechanism of aluminium-induced porphyrin synthesis in bacteria. Biometals 9, 73–77 (1996). https://doi.org/10.1007/BF00188093
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DOI: https://doi.org/10.1007/BF00188093