Skip to main content
Log in

Formation and dynamics of female and male pronuclei in the fertilization of the eggs of Rana limnocharis

  • Original Article
  • Published:
Anatomy and Embryology Aims and scope Submit manuscript

Abstract

Eggs of Rana limnocharis were inseminated with conspecific sperm and the formation and timing of the second polar body, the formation of female and male pronuclei, and their dynamic movements were observed. Sperm enter the egg at random locations around the second polar body, between 15° and 60°.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Elinson RP (1975) Site of sperm entry and a cortical contraction associated with egg activation in the frog Rana pipiens. Dev Biol 47:257–268

    Google Scholar 

  • Glover DM, Hames BD (1989) Genes and embryos. Oxford University Press, Oxford p 128

    Google Scholar 

  • Grey RD, Bastianti MJ, Webb DJ, Schertel ER (1982) An electrical block is required to prevent polyspermy in eggs fertilized by natural mating of Xenopus laevis. Dev Biol 89:475–484

    Google Scholar 

  • Hunter RHF (1972) Fertilization in the pig: sequence of nuclear and cytoplasmic events. J. Reprod Fertil 29:395–406

    Google Scholar 

  • Jura C, Krzysztofowicz A (1992) Inititation of embryonic development in Tetrodontophora bielanesis (Waga) [Collembola: Family] eggs: Meiosis, polyspermy, union of gametes and first cleavage. Int J Insect Morphol Embryol 21:87–94

    Google Scholar 

  • King WA, Bousquet D, Greve T, Betteridge KJ (1985) The pronuclei of bovine ova fertilized in vitro. Hereditas 102:293–296

    Google Scholar 

  • Labordus V, Hooftman RN, Wal UP van der (1986) The formation of the pronuclei and their associated perinuclear cytoplasm: The determination of the phases of the first cleavage cycles in Crepidula fornicate (Mollusca, Gastropoda). Int J Invert Reprod Dev 10:89–104

    Google Scholar 

  • Longo FJ, Anderson E (1968) The fine structure of pronuclear development and fusion in the sea urchin Arbacia punctulata. J Cell Biol 39:339–368

    Google Scholar 

  • Niwa K, Chang MC (1975) Fertilization of rat eggs in vitro at various times after ovulation with special reference to fertilization of ovarian oocytes matured in culture. J Reprod Fertil 43:435–451

    Google Scholar 

  • Niwa K, Imai H, Kim CI, Iritani A (1980) Fertilization in vitro of hamster and mouse eggs in a chemically defined medium. J Reprod Fertil 58:109–114

    Google Scholar 

  • Niwa K, Hosoi Y, Ohara K, Iritani A (1983) Fertilization in vitro of rabbit eggs with or without follicular cells by epididymal spermatozoa capacitated in a chemically defined medium. Anim Reprod Sci 6:143–149

    Google Scholar 

  • Niwa K, Ohara K, Hosoi Y, Iritani A (1985) Early events of in vitro fertilization of cat eggs by epididymal spermatozoa. J Reprod Fertil 74:657–660

    Google Scholar 

  • Sathananthan AH, Kola I, Osborne J, Trounson A, Ng SC (1991) Centrioles in the beginning of human development. Proc Natl Acad Sci USA 88:4806–4810

    Google Scholar 

  • Sele B, Pellestor F, Jalbert P, Estrade C, Ostoreor C, Gelas M (1985) Pronuclei cytogenetic analysis after interspecific manhamster fertilization. Ann Genet 28:81–85

    Google Scholar 

  • Stewart-Savage J, Grey RD (1982) The temporal and spatial relationships between cortical contraction, sperm trail formation, and pronuclear migration in fertilized Xenopus eggs. Roux's Arch Develop Biol 191:241–245

    Google Scholar 

  • Stewart-Savage J, Grey RD (1984) Fertilization of investment-free Xenopus eggs. Exp Cell Res 154:639–642

    Google Scholar 

  • Tchou-Su (1931) Elude cytologique sur l'hybridation chez les anoures. Arch Anat Microsc 27:1–105

    Google Scholar 

  • Tchou-Su, Bataillon E (1933) Les prodessus un'etiques dans l'oeuf de Bombyx mori (fecondation normale; parthenogenese; activation polyvobtinisante; dissociation experimentale des rythmes). Arch Anat Microsc Morphol Exp 29:285–372

    Google Scholar 

  • Tchou-Su, Chen ZX, Wang YL (1960) A cytological study of fertilisation of Carassius auratus and Megalobrama terminalis. Acta Biol Exp Sin 7:29–42

    Google Scholar 

  • Wu ZQ (1983) A cytological study on the formation of the gynogenetic haploid of Rana limnocharis. Zool Sin 29:295–299

    Google Scholar 

  • Xu Z, Ding H (1995) A study of embryonic cell metabolism of androgenetic haploid Rana limnocharis. Acta Herpetol Sin 45: 198–203

    Google Scholar 

  • Xu Z, Zhang K (1991) Androgenetic haploid produced by laser ablation of maternal chromosomes in eggs in Rana limnocharis. Appl Lasers 11:228–230

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, Z., Ding, H. Formation and dynamics of female and male pronuclei in the fertilization of the eggs of Rana limnocharis . Anat Embryol 193, 619–623 (1996). https://doi.org/10.1007/BF00187934

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00187934

Key words

Navigation