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Afferents to different layers of the dorsolateral isocortex in rats

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Abstract

Fluorescent somatopetal tracers were used to infiltrate, by diffusion rather than injections, the dorsolateral cortex of one hemisphere in rats. In different animals the tracers penetrated into the cortex to different depths. We found several interesting features of the commissural system: first, there were no areas without commissural neurons. At least a few labelled cell bodies were present in a single-cell layer also in “acallosal” cortical areas. Secondly, there is a considerable variety of laminar distribution patterns of labelled perikarya in different areas. Thirdly, some cortical fields, which cytoarchitecturally appear uniform, can be subdivided according to different distributions of cell bodies with commissural projections. Fourthly, when only supragranular layers were infiltrated, labelled cell bodies were present mainly in the supragranular layers of the contralateral cortex. Infiltration of the first layer alone did not label any neurons in the contralateral cortex but did label neurons in layer VIb ipsilaterally. In the subcortex, the labelled perikarya were found in the structures already known to project directly to the cortex. In rats with the tracer restricted mainly to the supragranular layers, a conspicuously reduced labelling was found in the basal forebrain and the thalamus. In the thalami of those animals, labelled neurons were found only in paralamellar nuclei. The high sensitivity of the tracer used, together with infiltration of the entire dorsolateral cortex, allows us to conclude that probably all sources of innervation of the isocortex in rats have been seen.

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References

  • Aschoff A, Holländer H (1982) Fluorescent compounds as retrograde tracers compared with horseradish peroxidase (HRP). I. A parametric study in the central visual system of the albino rat. J Neurosci Methods 6:179–197

    Google Scholar 

  • Audinat E, Condé F, Crépel F (1988) Cortico-cortical connections of the limbic cortex of the rat. Exp Brain Res 69:439–443

    Google Scholar 

  • Beckstead RM (1979) An autoradiographic examination of cortical and subcortical projections of the mediodorsal projection (prefrontal) cortex in the rat. J Comp Neurol 184:43–62

    Google Scholar 

  • Cowan WM, Gottlieb DI, Hendrickson AE, Price JL, Woolsey TA (1972) The autoradiographic demonstration of axonal connections in the central nervous system. Brain Res 37:21–51

    Google Scholar 

  • Cusick CG, Lund RD (1982) Modification of visual callosal projections in rats. J Comp Neurol 212:385–398

    Google Scholar 

  • De Olmos J, Heimer L (1980) Double and triple labelling of neurons with fluorescent substances: the study of collateral pathways in the ascending raphe system. Neurosci Lett 19:7–12

    Google Scholar 

  • Divac I (1975) Magnocellular nuclei of the basal forebrain project to neocortex, brain stem, and olfactory bulb. Review of some functional correlates. Brain Res 93:385–398

    Google Scholar 

  • Divac I (1981) Cortical projections of the magnocellular nuclei of the basal forebrain: a reinvestigation. Neuroscience 6:983–984

    Google Scholar 

  • Divac I, Mogensen J (1990) Long-term labelling of neurons. Brain Res 524:339–341

    Google Scholar 

  • Divac I, LaVail JH, Rakic P, Winston KR (1977) Heterogeneous afferents to the inferior parietal lobule of the rhesus monkey revealed by the retrograde transport method. Brain Res 123:197–207

    Google Scholar 

  • Divac I, Kosmal A, Björklund A, Lindvall O (1978) Subcortical projections to the prefrontal cortex in the rat as revealed by the horseradish peroxidase technique. Neuroscience 3:785–796

    Google Scholar 

  • Divac I, Petrovic-Minic B, Mogensen J (1984) Focal cortical seizures prevent HRP and HRP-WGA labeling only in neurons bidirectionally connected to the cortex. Brain Res 3:189–193

    Google Scholar 

  • Divac I, Marinkovic S, Mogensen J, Schwerdtfeger W, Regidor J (1987a) Vertical ascending connections in the isocortex. Anat Embryol 175:443–455

    Google Scholar 

  • Divac I, Mogensen J, Marinkovic S, Mårtensson R (1987b) On the projections from the neostriatum to the cerebral cortex: the “displaced” neurons. Neuroscience 21:197–205

    Google Scholar 

  • Dunker RO, Harris AB, Jenkins DP (1976) Kinetics of horseradish peroxidase migration through cerebral cortex. Brain Res 118:199–217

    Google Scholar 

  • Herkenham M (1979) The afferent and efferent connections of the ventromedial thalamic nucleus in the rat. J Comp Neurol 183:487–518

    Google Scholar 

  • Herkenham M (1986) New perspectives on the organization and evolution of nonspecific thalamocortical projections. In: Jones EG, Peters A (eds) Cerebral cortex, vol 5. Plenum Press, New York, pp 403–445

    Google Scholar 

  • Hübener M, Boltz J (1988) Morphology of identified neurons in layer 5 of rat visual cortex. Neurosci Lett 94:76–81

    Google Scholar 

  • Innocenti GM (1986) General organization of callosal connections in the cerebral cortex. In: Jones EG, Peters A (eds) Cerebral cortex, vol V. Plenum Press, New York, pp 291–353

    Google Scholar 

  • Ivy GO, Gould HJ III, Killackey HP (1984) Variability in the distribution of callosal projection neurons in the adult rat parietal cortex. Brain Res 306:53–61

    Google Scholar 

  • Jacobson S, Trojanowski JQ (1974) The cells of origin of the corpus callosum in rat, cat and rhesus monkey. Brain Res 74:149–155

    Google Scholar 

  • Jones EG (1975) Possible determinants of the degree of retrograde neuronal labelling with horseradish peroxidase. Brain Res 85:249–253

    Google Scholar 

  • Jouandet ML, Garey LJ, Lipp H-P (1984) Distribution of the cells of origin of the corpus callosum and anterior commissure in the marmoset monkey. Anat Embryol 169:45–59

    Google Scholar 

  • Jouandet ML, Lachat J-J, Garey LJ (1985) Distribution of the neurons of origin of the great cerebral commissures in the cat. Anat Embryol 171:105–120

    Google Scholar 

  • Jouandet ML, Lachet J-J, Garey LJ (1986) Topographic distribution of callosal neurons and terminals in the cerebral cortex of the cat. Anat Embryol 173:323–342

    Google Scholar 

  • Kristensson K (1970) Transport of fluorescent protein tracer in peripheral nerves. Acta Neuropathol (Berl) 16:293–300

    Google Scholar 

  • Kuypers HGJM, Huisman AM (1984) Fluorescent neuronal tracers. In: Advances in cellular neurobiology, vol 5. Academic Press, New York, pp 307–340

    Google Scholar 

  • LaVail JH, LaVail MM (1972) Retrograde axonal transport in the central nervous system. Science 176:1416–1417

    Google Scholar 

  • Leichnetz GR (1982) Connections between the frontal eye field and pretectum in the monkey. An anterograde-retrograde study using HRP gel and TMB neurohistochemistry. J Comp Neurol 207:392–402

    Google Scholar 

  • Lipp HP, Schwegler H (1980) Improved transport of horseradish peroxidase after injection with a non-ionic detergent (Nonidet p-40) into mouse cortex and observations on the relationship between spread at the injection site and amount of transported label. Neurosci Lett 20:49–54

    Google Scholar 

  • Luiten PGM, Gaykama RPA, Traber J, Spencer DG Jr (1987) Cortical projection patterns of magnocellular basal nucleus subdivisions as revealed by anterogradely transported Phaseolus vulgaris leucoagglutinin. Brain Res 413:229–250

    Google Scholar 

  • Markowitsch HJ, Guldin WO (1983) Heterotopic interhemispheric cortical connections in the rat. Brain Res Bull 10:805–810

    Google Scholar 

  • Mårtensson R, Björklund A (1984) Low-power photography in the fluorescence microscope using an automatic dark-field condenser-scanner. In: Björklund A, Hökfelt T (eds) Handbook of chemical neuroanatomy, vol 2. Classical transmitters in the CNS. Part 1. Elsevier, Amsterdam, pp 380–386

    Google Scholar 

  • McBride RL, Feringa ER, Garver MK, Williams JK Jr (1990) Retrograde transport of Fluoro-Gold in corticospinal and rubrospinal neurons 10 and 20 weeks after T-9 spinal cord transsection. Exp Neurol 108:83–85

    Google Scholar 

  • Mercier BE, Legg CR, Glickstein M (1990) Basal ganglia and cerebellum receive different somatosensory information in rats. Proc Natl Acad Sci USA 87:4388–4392

    Google Scholar 

  • Miller MW, Vogt BA (1984) Heterotopic and homotopic callosal connections in rat visual cortex. Brain Res 297:75–89

    Google Scholar 

  • Olavarria J, Van Sluyters RC (1983) Widespread callosal connections in infragranular visual cortex of the rat. Brain Res 279:233–237

    Google Scholar 

  • Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates, 2nd edn. Academic Press, Sydney

    Google Scholar 

  • Peyronnard J-M, Charron L (1983) Decreased horseradish peroxidase labelling in deafferented spinal motoneurons of the rat. Brain Res 275:203–214

    Google Scholar 

  • Preuss TM, Goldman-Rakic P (1987) Crossed corticothalamic and thalamocortical connections of macaque prefrontal cortex. J Comp Neurol 257:269–281

    Google Scholar 

  • Ribak CE (1977) A note on the laminar organization of rat visual cortical projections. Exp Brain Res 27:413–418

    Google Scholar 

  • Rieck RW, Carey RG (1985) Organization of the rostral thalamus in the rat: Evidence for connections to layer I of visual cortex. J Comp Neurol 234:137–154

    Google Scholar 

  • Rüttgers K, Aschoff A, Frianf E (1990) Commissural connections between the auditory cortices of the rat. Brain Res 509:71–79

    Google Scholar 

  • Sarter M, Markowitsch HJ (1985) Convergence of intra- and interhemispheric cortical afferents: lack of collateralization and evidence for a subrhinal cell group projecting heterotopically. J Comp Neurol 236:283–296

    Google Scholar 

  • Schmued LC (1990) Fluoro-Gold and 4-acetamido-4′-isothiocyanostilbene-2, 2′-disulfonic acid: use of substituted stilbenes in neuroanatomical studies. In: Conn PM (ed) Quantitative microscopy. Methods in neuroscience, vol 3. Academic Press, New York, pp 317–330

    Google Scholar 

  • Schmued LC, Fallon JH (1986) Fluoro-Gold: a new fluorescent retrograde axonal tracer with numerous unique properties. Brain Res 337:147–154

    Google Scholar 

  • Singer W, Holländer H, Vanegas H (1977) Decreased peroxidase labelling of lateral geniculate neurons following deafferentation. Brain Res 120:133–137

    Google Scholar 

  • Vanegas H, Holländer H, Distel H (1978) Early stages of uptake and transport of horseradish peroxidase by cortical structures, and its use for the study of local neurons and their processes. J Comp Neurol 177:193–212

    Google Scholar 

  • Wiley RG, Blessing WW, Reis DJ (1982) Suicide transport: destruction of neurons by retrograde transport of ricin, abrin and modeccin. Science 216:889–890

    Google Scholar 

  • Wise SP, Jones EG (1976) The organization and postnatal development of the commissural porjection of the rat somatosensory cortex. J Comp Neurol 168:313–343

    Google Scholar 

  • Zaborsky L, Wolff JR (1982) Distribution patterns and individual variations of callosal connections in the albino rat. Anat Embryol 165:213–232

    Google Scholar 

  • Zilles K (1985) The cortex of the rat. A stereotaxic atlas. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Zilles K (1990) Anatomy of the neocortex: cytoarchitecture and myeloarchitecture. In: Kolb B, Tees RC (eds) The cerebral cortex of the rat. MIT Press, Cambridge, USA, pp 77–112

    Google Scholar 

  • Zilles K, Zilles B, Schleicher A (1980) A quantitative approach to cytoarchitectonics. VI. The areal pattern of the cortex of the albino rat. Anat Embryol 159:335–360

    Google Scholar 

  • Zilles K, Wree A, Schleicher A, Divac I (1984) The monocular and binocular subfields of the rat's primary visual cortex. A quantitative morphological approach. J Comp Neurol 226:391–402

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Medical Physiology, Panum Institute, University of Copenhagen, DK-2200, Copenhagen, Denmark

    Ivan Divac, Slobodan Milosevic & Jesper Mogensen

  2. Department of Morphology, Faculty of Health Sciences, University of Las Palmas de Gran Canaria, Spain

    Jose Regidor

  3. Laboratory of Neuropsychiatry, Department of Pharmacology, University of Copenhagen, Denmark

    Jesper Mogensen

  4. C. and O. Vogt Institute for Brain Research, University of Düsseldorf, Germany

    Karl Zilles

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  1. Ivan Divac
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  2. Jose Regidor
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  3. Slobodan Milosevic
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  4. Jesper Mogensen
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  5. Karl Zilles
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Divac, I., Regidor, J., Milosevic, S. et al. Afferents to different layers of the dorsolateral isocortex in rats. Anat Embryol 192, 63–75 (1995). https://doi.org/10.1007/BF00186992

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