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Expression of the chicken retinoid X receptor-γ gene in migrating cranial neural crest cells

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Abstract

We have used in situ hybridisation to whole chick embryos with digoxygenin-labelled probes to investigate the distribution of RXR-γ transcripts during neural crest cell migration in the developing head and the anterior of the trunk (the vagal region), where neural crest cells make a substantial contribution. We have found that RXR-γ transcripts are a good marker for migrating neural crest cells in the chick embryo. RXR-γ transcripts were first detected in cells that had recently emerged from the neural crest, providing an earlier marker for neural crest cells than the HNK-1 epitope. The pattern of RXR-γ transcript distribution is dynamic in the developing chick head, and changes in a pattern which is coincident with the migration of cells containing RXR-γ transcripts and the gradual restriction of RXR-γ transcripts to specific differentiating neural crest derivatives. Transcripts appeared to be present initially in migrating neural crest cells thoughout the developing head, but gradually became restricted to some crest-derived populations and absent from others. By stage 15, RXR-γ transcripts were not detectable in neural-crest-derived ectomesenchymal cells, although they were still found in cells contributing to the cranial ganglia and their roots.

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  1. Annie Rowe

    Present address: START Laboratory, Chelsea and Westminster Hospital, Fulham Road, SW10 9NH, London, UK

Authors and Affiliations

  1. Department of Anatomy and Developmental Biology, University College London Medical School, The Windeyer Building, Cleveland Street, W1P 6DB, London, UK

    Annie Rowe

  2. Medical Molecular Biology Unit, Department of Molecular Pathology, University College London Medical School, The Windeyer Building, Cleveland Street, W1P 6DB, London, UK

    Annie Rowe & Paul M. Brickell

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  1. Annie Rowe
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  2. Paul M. Brickell
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Rowe, A., Brickell, P.M. Expression of the chicken retinoid X receptor-γ gene in migrating cranial neural crest cells. Anat Embryol 192, 1–8 (1995). https://doi.org/10.1007/BF00186986

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