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Dynamics of Na,K-ATPase sites in lateral cochlear wall tissues of the rat

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Summary

The objective of this study was to determine whether varying levels of either the synthetic glucocorticoid, dexamethasone, or the mineralocorticoid, aldosterone, modulate the quantity of Na,K-ATPase sites in stria vascularis and spiral ligament tissues. Surgically adrenalectomized male rats were administered different dosages of dexamethasone or aldosterone for 7 days and subsequently were sacrificed. Na,K-ATPase α-subunits of stria vascularis and spiral ligament homogenates were determined quantitatively by enzyme-linked immunosorbent assay, using a monoclonal antibody shown to react with lateral wall Na,K-ATPase α-subunit. Elevated serum levels of dexamethasone were correlated with significantly increased Na,K-ATPase α-subunit levels in both the stria vascularis and spiral ligament (P 〈 0.05). Elevated serum levels of aldosterone were correlated with increased Na,K-ATPase α-subunits in the stria vascularis, but not in the spiral ligament (P 〈 0.1). These data further indicate a positive correlation between increased serum levels of adrenal steroids and induction of Na,K-ATPase synthesis by such steroids; particularly by dexamethasone.

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Authors and Affiliations

  1. Departments of Anatomy & Cell Biology and Otolaryngology, College of Medicine, University of Florida, 32610, Gainesville, FL, USA

    L. M. Curtis, W.-J. F. ten Cate & K. E. Rarey

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  1. L. M. Curtis
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  2. W.-J. F. ten Cate
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  3. K. E. Rarey
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Curtis, L.M., ten Cate, WJ.F. & Rarey, K.E. Dynamics of Na,K-ATPase sites in lateral cochlear wall tissues of the rat. Eur Arch Otorhinolaryngol 250, 265–270 (1993). https://doi.org/10.1007/BF00186223

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  • DOI: https://doi.org/10.1007/BF00186223

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