Summary
The effect of low-energy laser (He-Ne) irradiation on the rate of skeletal muscle regeneration after partial excision of the rat gastrocnemius muscle was studied using quantitative histological morphometric methods. The injured zones of the experimental rats were subjected to direct He-Ne laser (632.8 nm wavelength) irradiation (6.0 mW for 2.3 min) immediately following injury and once daily thereafter for 5 days. Muscles that were injured as above and subjected to red or room light irradiation served as a control group. The volume fraction (percent of total volume of injured zone) of the mononucleated cells in the injured zone decreased gradually with time after injury, but more rapidly in the laser irradiated muscles than in the control. At 3 days post-injury the myotubes in the laser-irradiated muscles populated a significantly higher percentage (13.9 ± 1.1%) of the injured area than in the control muscles (7.8 ± 1.0%). The volume fraction of the young myofibers in the laser irradiated muscles exceeded 30.6 ± 2.2% and 49.6 ± 5.6% at 8 and 11 days post-injury, respectively, while in control muscles these structures comprised only 9.6 ± 1.0% and 27.2 ± 3.8% of the injured zone at 8 and 11 days after injury, respectively. It is concluded that He-Ne laser irradiation during the regeneration process promotes muscle maturation in the injured zone following partial excision of the rat gastro-cnemius muscle.
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References
Allbrook D (1981) Skeletal muscle regeneration. Muscle Nerve 4:234–245
Assia E, Rosner M, Belkin M, Solomon A, Schwartz M (1989) Temporal parameters of low energy laser irradiation for optimal delay of post-traumatic degeneration of rat optic nerve. Brain Res 476:205–212
Belkin M, Zaturunsky B, Schwartz M (1988) A critical review of low energy laser bioeffects. Lasers Light Ophthalmol 2:63–71
Bischoff R (1986a) Proliferation of muscle satellite cells on intact myofibers in culture. Dev Biol 115:129–139
Bischoff R (1986b) A satellite cell mitogen from crushed adult muscle. Dev Biol 115:140–147
Bischoff R (1990) Cell cycle commitment of rat muscle satellite cells. J Cell Biol 111:201–207
Carlson BM, Faulkner JA (1983) The regeneration of skeletal muscle fiber following injury. A review. Med Sci Sports Exerc 15:107–198
Carrino DA, Oron U, Pechak DG, Caplan AI (1988) Reinitiation of chondroitin sulphate proteoglycan synthesis in regenerating skeletal muscle. Development 103:641–656
Karu T (1989) Photobiology of low-power laser effects. Health Phys 56:691–704
Kovacs IB, Mester E, Gorog P (1974) Stimulation of normal healing with laser beam in the rat. Experientia 30:1275–1280
Loud AV, Anversa P (1984) Biology of disease: morphometric analysis of biological processes. Lab Invest 50:250–261
Maltz L, Oron U (1990) Proteolytic enzyme activities during regeneration of the rat gastrocnemius muscle. J Neurol Sci 98:149–154
Mathieu O, Cruz-Orive LM, Hoppeler H, Weibel ER (1981) Measuring error and sampling variation in stereology: comparison of the efficiency of various methods for planar image analysis. J Microsc 121:75–88
Mester E, Spiry T, Sjende B (1973) Effect of low laser rays in wound healing. Bull Soc Int Chir 2:169–181
Oron U (1984) Acetylcholinesterase and nerve axon formation during muscle regeneration in rats. Cell Mol Biol 30:411–416
Oron U (1990) Proteolytic enzyme activity in rat hindlimb muscles in fetus and during post-natal development. Int J Dev Biol 34:457–460
Passarella S, Casamassima E, Molinari S, Pastore D, Quagliariello E, Catalano IM, Cingolani A (1984) Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by Helium-Neon laser. FEBS Lett 175:95–99
Phillips GD, Knighton DR (1990) Angiogenic activity in damaged skeletal muscle. Proc Soc Exp Biol Med 193:197–202
Plaghki L (1985) Regeneration et myogenese du muscle strié. J Physiol (Paris) 80:51–110
Rochkind S, Barr Nea L, Bartal A, Nissan M, Lubart R, Razon N (1988) New methods of treatment of severely injured sciatic nerve and spinal cord. Acta Neurochir [Suppl] 43:91–93
Roth D, Oron U (1985) Repair mechanisms involved in muscle regeneration following partial excision of the rat gastrocnemius muscle. Exp Cell Biol 53:107–114
Schwartz M, Doron A, Erlich M, Lavie V, Benbasat S, Belkin M, Rochkind S (1987) Effect of low energy He-Ne laser irradiation on post-traumatic degeneration of adult rabbit optic nerve. Lasers Surg Med 7:51–55
Sokal RR, Rohlf FG (1981) Biometry, 2nd edn. Freeman, London
Weibel ER (1979) Stereological methods: practical methods for biological morphometry. Academic Press, London
Weibel ER (1980) Stereological methods: theoretical foundations. Academic press, London
Weiss N, Oron U (1991) Enhancement of repair process in gastrocnemius muscle in the rat by electromagnetic irradiation. Proceedings of 28th Meeting of Zoological Society of Israel, Tel Aviv, p 28
White TP, Esser AK (1989) Satellite cell and growth factor involvement in skeletal muscle growth. Med Sci Sports Exerc 21:S158-S163
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Weiss, N., Oron, U. Enhancement of muscle regeneration in the rat gastrocnemius muscle by low energy laser irradiation. Anat Embryol 186, 497–503 (1992). https://doi.org/10.1007/BF00185463
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DOI: https://doi.org/10.1007/BF00185463