Skip to main content
Log in

Canine and feline haematology analysis: Comparative performance of technicon H*1 and AVL MS8 VET analysers

  • Original Article
  • Published:
Comparative Haematology International Aims and scope Submit manuscript

Abstract

The performance of a prototype AVL MS8 VET impedance haematology analyser was compared with that of a Technicon H*1 flow cytometry haematology analyser using blood from dogs and cats. Analysis was performed with the AVL MS8 VET on the day of blood collection and with the Technicon H*1 on the following day. Differences were noted in feline leucocyte and platelet counts and in canine and feline mean cell volume and mean cell haemoglobin concentration between analyses. The results indicate that the AVL MS8 VET is a reliable analyser for blood samples from dogs but may not be for those from cats. Attention is drawn to the importance of considering the type of analyser, calibration of the analyser, time of analysis after blood collection (effect of postal delay) and the effect of anticoagulants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Brittin GM, Brecher G, Johnson CA (1969) Evaluation of the Coulter Counter model S. Am J Clin Pathol 52:679–689

    Google Scholar 

  • Davies DT, Fisher GV (1991) The validation and application of the Technicon H*1 for the complete automated evaluation of laboratory animal haematology. Comp Haematol Int 1:91–105

    Google Scholar 

  • Ghanayem BI, Ward SM, Blair PC et al. (1990) Comparison of the haematologic effects of 2-butoxyethanol using two types of haematology analysers. Toxicol Appl Pharmacol 106:341–345

    Google Scholar 

  • Hinchliffe RF, Bellamy GJ, Lilleyman JS (1992) Use of the Technicon H*1 hypochromia flag in detecting spurious macrocytosis induced by excessive K2-EDTA concentration. Clin Lab Haematol 14:268–269

    Google Scholar 

  • Jain NC (1986) Schalm's Veterinary Haematology. Fourth edn. Lea & Febiger, Philadelphia

    Google Scholar 

  • Kimball JP, Sansone J, Lewandowski AD et al (1992) Multispecies haematology analysis: comparative performance of Technicon H*1E and Baker 9000 haematology analysers for lab animals. Proc V Congress of Int Soc of Anim Biochem, Parma, Italy

  • Levin J, Bessman JD (1983) The inverse relation between platelet volume and platelet number. J Lab Clin Med 101:295–307

    Google Scholar 

  • Lippi U, Schinella M, Modena N et al. (1987) Unpredictable effects of K3EDTA on mean platelet volume. Am J Clin Pathol 87:391–393

    Google Scholar 

  • Lippi U, Schinella M, Modena N et al. (1990) Advantages of a new anticoagulant in routine haematology on the Coulter Counter® S-Plus STKR analyser. Am J Clin Pathol 93:760–764

    Google Scholar 

  • Onder O, Weinstein A, Hoyer LW (1980) Pseudothrombocytopenia caused by platelet agglutinins that are reactive in blood anticoagulated with chelating agents. Blood 56:177–182

    Google Scholar 

  • Pinkerton PH, Spence I, Olgilvie JC et al. (1970) An assessment of the Coulter Counter model S. Vet Clin Pathol 23:68–76

    Google Scholar 

  • Reardon DM, Hutchinson D, Preston FE et al. (1985) The routine measurement of platelet volume: a comparison of aperture-impedance and flow cytometric systems. Clin Lab Haematol 7:251–257

    Google Scholar 

  • Savage RA (1984) Pseudoleukocytosis due to EDTA-induced platelet clumping. Am J Clin Pathol 81:317–322

    Google Scholar 

  • Thompson CB, Diaz DD, Quinn PG et al. (1983) The role of anticoagulation in the measurement of platelet volumes. Am J Clin Pathol 80:327–332

    Google Scholar 

  • Weingand KW, Odioso LW, Dameron GW et al. (1992) Haematology analyser comparison: Ortho ELT-8/ds vs. Baker 9000 for healthy dogs, mice and rats. Vet Clin Pathol 21:10–14

    Google Scholar 

  • Weiser MG (1983) Comparison of two automated multi-channel blood cell counting systems for analysis of blood of common domestic animals. Vet Clin Pathol 12:25–32

    Google Scholar 

  • Weiser MG (1987) Modification and evaluation of a multichannel blood cell counting system for blood analysis in veterinary haematology. J Am Vet Med Assoc 190:411–415

    Google Scholar 

  • Zelmanovic D, Sansone J, Kimball JP (1992) Sources of error affecting multispecies haematology RBC counting accuracy. Proc V Congress of the Int Soc of Anim Biochem, Parma, Italy

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Byrne, R.F., AndreW, C.M., Libretto, S.E. et al. Canine and feline haematology analysis: Comparative performance of technicon H*1 and AVL MS8 VET analysers. Comp Haematol Int 4, 212–217 (1994). https://doi.org/10.1007/BF00185176

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00185176

Keywords

Navigation