Abstract
We have studied the properties of kainic acid receptor-activated channels using domoic acid as an agonist. Similarities of the electrophysiological, pharmacological and noise properties of domoic acid and kainic acid-evoked currents confirm that domoate is a potent and specific agonist of the kainate receptor. Single-channel properties of domoic acid-evoked currents were directly determined from outside-out membrane patches for the first time, and results were compared with those obtained by fluctuation analysis of macroscopic currents. Small conductance cationic-selective channels of ≈4 pS and a mean open time of 2 to 3 ms were detected using both methods.
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Sciancalepore, M., Galdzicki, Z., Zheng, X. et al. Kainate activated single channel cnrrents as revealed by domoic acid. Eur Biophys J 19, 63–68 (1990). https://doi.org/10.1007/BF00185087
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DOI: https://doi.org/10.1007/BF00185087