Summary
Chlamydomonas reinhardtii cells immobilized in Ba-alginate beads provide a stable and effective system for photoproducing ammonium from nitrite in a culture medium containing l-methionine-d,l-sulphoximine. The process was studied in either air-lift, fluidized or packed-bed reactors, the last one providing the most suitable system with a volumetric activity of 2700 μmol NH sup4inf+ ·1−1 per hour.
Similar content being viewed by others
References
Arnon DI (1949) Copper enzymes in isolated chloroplasts: polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15
Brodelius P (1985) The potential role of immobilization in plant cell biotechnology. Trend Biotechnol 3:280–285
Brodelius P, Nilsson K (1980) Entrapment of plant cells in different matrices. FEBS Lett 122:312–316
Brouers M, Hall DO (1986) Ammonia and hydrogen production by immobilized cyanobacteria. J Biotechnol 3:307–321
Cheetham PSJ, Kevin WB, Buche C (1979) Physical studies on cell immobilization using calcium alginate gels. Biotechnol Bioeng 21:2155–2168
Dainty AL, Goulding KH, Robinson PK, Simpkins I, Trevan MD (1986) Stability of alginate immobilized algae cells. Biothchnol Bioeng 28:210–216
Fernández E, Matagne R (1984) Genetic analysis of nitrate reductase deficient mutants in Chlamydomonas reinhardtii. Curr Genet 8:635–640
Florencio FJ, Vega JM (1983) Utilization of nitrite, nitrite and ammonium by Chlamydomonas reinhardtii. Photoproduction of ammonium. Planta 158:288–293
Kennedy FJ, Cabral JMS (1983) Immobilized enzymes In: Scouten WH (ed) Solid phase biochemistry. Analytical and synthetic aspects. Wiley, New York, pp 253–368
Levenspiel O (1979) The chemical reactor omnibook. Chemical Engineering Department, University of Oregon State
Musgrave SC, Kerby NW, Codd G, Stewart WDP (1982) Sustained ammonia production by immobilized filaments of the nitrogen fixing cyanobcteria Anabaena 27893. Biotechnol Lett 4:647–651
Paul F, Vignais PM (1980) Photophosphorylation in bacterial chromatophores entrapped in alginate gel: improvement of the physical and biochemical properties of the gel beads with barium as gel-inducing agent. Enzyme Microbiol Technol 2:281–287
Robinson PK, Mak AL, Trevan MD (1986) Immobilized algae: a review. Process Biochem. August: 122–127
Santos-Rosa F, Galván F (1989) Ammonium photoproduction by free and immobilized cells of Chlamydomonas reinhardtii. Appl Microb Biotechnol 31:55–58
Snell FD, Snell CT (1949) Colorimetric method of analysis, vol. 2. Van Nostrand, New York, pp 804–805
Solorzano L (1969) Determination of ammonia in natural waters by the phenolhypochlorite method. Limnol Oceanogr 14:799–801
Sueoka N, Chian KS, Kates JR (1967) Deoxyribonucleic acid replication in meiosis of Chlamydomonas reinhardtii. I. Isotopic transfer experiments with a strain producing eight zoospores. J Mol Biol 25:47–66
Trevan MD, Mak AL (1988) Immobilized algae and their potential for use as biocatalysts. Trends Biotechnol 6:68–72
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Santos-Rosa, F., Galván, F. & Vega, J.M. Photoproduction of ammonium by Chlamydomonas reinhardtii cells immobilized in barium alginate: a reactor feasibility study. Appl Microbiol Biotechnol 32, 285–290 (1989). https://doi.org/10.1007/BF00184975
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00184975