Production of native-starch-degrading enzymes by a Bacillus firmus/lentus strain

Summary

A bacterium belonging to the Bacillus firmus/lentus-complex and capable of growth on native potato starch was isolated from sludge of a pilot plant unit for potato-starch production. Utilization of a crude enzyme preparation obtained from the culture fluid after growth of the microorganism on native starch, resulted in complete degradation of native starch granules from potato, maize and wheat at a temperature of 37°C. Glucose was found as a major product. Production of maltose, maltotriose and maltotetraose was also observed. Native-starch-degrading activity (NSDA) could be selectively adsorbed on potato-starch granules, whereas soluble-starch-degrading activity (SSDA) remained mainly in solution. The use of such a starch-adsorbed enzyme preparation on native starch resulted in a completely changed product pattern. An increase in oligosaccharides concomitant with less glucose formation was observed. An increased conversion of soluble starch to maltopentaose was possible with this starch-adsorbed enzyme preparation. It is concluded that NSDA comes from α-amylase(s) and SSDA from glucoamylase(s) and/or α-glucosidase(s). Cultivation of B. firmus/lentus on glucose, maltose, or soluble starch resulted in substantially smaller quantities of (native) starch-degrading activity.

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References

  1. Abe J, Bergmann W, Obata K, Hizukuri S (1988) Production of the raw-starch digestion amylase of Aspergillus sp. K-27. Appl Microbiol Biotechnol 27:447–450

    Google Scholar 

  2. Brunt K (1982) Rapid separation of linear and cyclic glucooligosaccharides on a cation exchange resin using Ca2+-EDTA solution as eluent. J Chromatogr 246:145–151

    Google Scholar 

  3. Buchanan RE, Gibbons NE (ed) (1974) Bergey's manual of determinative bacteriology, 8th edn Williams and Wilkins, Baltimore

    Google Scholar 

  4. Buranakari L, Ito K, Izaki K, Takahashi H (1988) Purification and characterization of a raw starch-digestive amylase from non-sulfur purple photosynthetic bacterium. Enzyme Microb Technol 10:173–179

    Google Scholar 

  5. Dische Z (1962) Color reactions of hexoses. In: Whistler RL, Wolfrom ML, BeMiller JN, Shafizadeh F (ed) Methods in carbohydrate chemistry, Vol I. Academic Press, New York, pp 490–491

    Google Scholar 

  6. Fogarty WM (1983) Microbial amylases. In: Fogarty WM (ed) Microbial enzymes and biotechnology. Applied Science Publishers, London, pp 1–93

    Google Scholar 

  7. Hayashida S (1975) Selective submerged production of three types of glucoamylases by a black koji mold. Agric Biol Chem 39:2093–2099

    Google Scholar 

  8. Hayashida S, Teramoto I, Inoue T (1988) Production and characteristics of raw-potato-starch digesting α-amylase from Bacillus subtilis 65. Appl Environ Microbiol 54:466–472

    Google Scholar 

  9. Kelly CT, Moriarty M, Fogarty WM (1983) α-Glucosidase of Bacillus firmus. Biotechnol Lett 5:289–294

    Google Scholar 

  10. Matsuoka H, Koba Y, Ueda S (1982) Alcoholic fermentation of sweet potato without cooking. J Ferment Technol 60:599–602

    Google Scholar 

  11. Meers JL (1972) The regulation of α-amylase production in Bacillus licheniformis. Anthonie van Leeuwenhoek 38:585–590

    Google Scholar 

  12. Monma M, Kagei N, Kainuma K (1989) Raw starch digestion by Chalara paradoxa immobilized in calcium alginate. Starch 41:355–357

    Google Scholar 

  13. Nelson N (1952) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153:376–380

    Google Scholar 

  14. Sata H, Taniguchi H, Maruyama Y (1987) Regulation of amylase synthesis in Bacillus circulans F-2. Agric Biol Chem 51:1521–1527

    Google Scholar 

  15. Sawada M, Kurusawa K, Sasaki H, Takao S (1988) Method for direct saccharification of raw starch using enzyme produced by a basidiomycete belonging to the genus Corticium. US patent no. 4727026

  16. Tanaka T, Ishimoto E, Shimomura Y, Taniguchi M (1987) Purification and some properties of raw starch-binding amylase of Clostridium butyricum T7 isolated from mesophilic methane sludge. Agric Biol Chem 51:399–405

    Google Scholar 

  17. Tani Y, Fuji A, Nishise H (1988) Production of raw cassava starch-digestive glucoamylase by a 2-deoxyglucose resistant mutant of Rhizopus sp. J Ferment Technol 66:545–551

    Google Scholar 

  18. Taniguchi H, Odashima F, Igarishi M, Maruyama Y, Nakamurav M (1982) Characterization of a potato starch-digesting bacterium and its production of amylase. Agric Biol Chem 46:2107–2115

    Google Scholar 

  19. Ueda S, Zenin CT, Monteiro DA, Park YK (1981) Production of ethanol from raw cassava starch by a nonconventional fermentation method. Biotechnol Bioeng 23:291–299

    Google Scholar 

  20. Yoshigi N, Chicano T, Kamimura M (1985) Purification and properties of an amylase from Bacillus cereus NY-14. Agric Biol Chem 49:3369–3376

    Google Scholar 

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Wijbenga, DJ., Beldman, G., Veen, A. et al. Production of native-starch-degrading enzymes by a Bacillus firmus/lentus strain. Appl Microbiol Biotechnol 35, 180–184 (1991). https://doi.org/10.1007/BF00184683

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Keywords

  • Starch
  • Sludge
  • Maltose
  • Starch Granule
  • Soluble Starch