Summary
From 1986 until 1990, in vivo proliferation rates (PRs) in 110 patients with renal-cell carcinoma (RCC) were immunohistochemically determined by the Ki-67 assay. It could be demonstrated that the PRs of RCCs range between only 1% and 15%. Due to its low proliferative kinetics in vivo, tumor cytogenetic investigations of this malignancy remain rare. During short-term in vitro culture, the PRs of this neoplasm increased (21%–82%). Therefore, 36 untreated human RCCs were cultured in vitro for cytogenetic analysis using the G-banding technique. In all, 77.8% (28/36) of the renal malignancies investigated exhibited an aberration of chromosome 3, which seems to serve as a marker for this malignancy. Whereas tumor stage showed no correlation with PR, tumor grade exhibited a strong correlation with this parameter. According to the data presented herein, immunohistochemical determination of the tumor-specific PR using the monoclonal antibody Ki-67 is a practicable, reliable and reproducible method that complements conventional histological tumor grading and staging. This parameter appears to be useful in identifying RCC patients at high risk, especially at early stages that are identical in tumor stage and grade.
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References
Berger CS, Sandberg A, Todd IA (1986) Chromosomes in kidney, ureter, and bladder cancer. Cancer Genet Cytogenet 23:1–24
Caspersson T, Zech L, Johannsson C, Modest EJ (1970) Identification of human chromosomes by DNA-binding fluorescent agents. Chromosoma 30:215–227
Cohen AJ, Li FP, Berg S, Marchetto DJ, Tsai S, Jacobs SC, Brown RS (1979) Hereditary renal cell carcinoma associated with a chromosomal translocation. N Engl J Med 301.592–595
Cordell JL, Falini B, Erber NW, Ghosh AK, Abdulaziz Z, MacDonald S, Pulford KAF, Stein H, Mason DY (1984) Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219–229
Gerdes J, Schwab U, Lemke H, Stein H (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 31:13–20
Gerdes J, Dallenbach F, Lennert K (1984) Growth fractions in malignant non-Hodgkin's lymphomas (NHL) as determined in situ with the monoclonal antibody Ki-67. Hematol Oncol 2:365–371
Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H (1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol 133:1710–1715
Girod C (1986) Die Entwicklung der Kenntnisse von der Zelle. In: Toellner R (ed) Illustrierte Geschichte der Medizin. Rabe, Stuttgart, pp 1884–1893
Hermanek P, Sobin LH (1987) Classification of urological tumors. In: Hermanek P, Sobin LH (eds) TNM classification of malignant tumors, 47th edn. Springer, Berlin Heidelberg New York, pp 137–139
Hermanek P, Sigl A, Chlepas S (1976) Histological grading of renal cell carcinoma. Eur Urol 2:189–191
ISCN (1985) An international system of human cytogenetic nomenclature. Karger, Basel
Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibodies of a predefined specificity. Nature 256:495
Kovacs G, Szücs S, Riese W de, Baumgärtel I (1987) Specific chromosome aberration in human renal cell carcinoma. Int J Cancer 40:171–178
Lelle RJ, Heidenreich W, Stauch G, Gerdes J (1987) The correlation of growth fractions with histologic grading and lymph node status in human mammary carcinoma. Cancer 59:83–88
Rabes H, Carl P, Meister P, Rattenhuber U (1979) Analysis of proliferative compartments in renal adenocarcinoma. Cancer 44:799–804
Riese W de, Allhoff E, Lenis G, Liedke S, Feretos P, Jonas U (1989) Bestimmung der Proliferationstrate in-vivo sowie in-vitro beim Nierenzellkarzinom mittels des Ki-67-Assays. (In vivo and in vitro determination of the proliferation rates in renal cell carcinoma with the Ki-67-assay) Aktuel Urol 20: 79–84
Riese W de, Allhoff E, Pohl U, Lenis G, Liedke S, Atay Z, Jonas U, Warnaar SO (1989) Comparison of human normal and malignant renal cells in vivo and in vitro using cytological, cytochemical and immunocytochemical methods. Invest Urol 3:8–16
Schiodt T (1966) Breast carcinoma — a histological and prognostic study of 650 followed-up cases. Munksgaard, Copenhagen
Schorn A, Marberger M (1984) Long-term survival of untreated bilateral renal cell carcinoma with supradiaphragmatic vena caval thrombus. J Urol 131:108–109
Seabright M (1971) A rapid banding technique for human chromosomes. Lancet 11:971–972
Sumner AT, Evans HJ, Buckland RA (1971) New technqiue distinguishing between human chromosomes. Nature 232:31–32
Yoshida MA, Ohyashiki K, Ochi H, Gibas Z, Pontes JE, Prout GR, Sandberg AA (1986) Cytogenetic studies of tumor tissue from patients with nonfamilial renal cell carcinoma. Cancer Res 46:2139–2147
Yunis JJ (1983) The chromosomal basis of human neoplasia. Science 221:227–236
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de Riese, W., Allhoff, E., Werner, M. et al. Proliferative behaviour and cytogenetic changes in human renal-cell carcinoma. World J Urol 9, 79–85 (1991). https://doi.org/10.1007/BF00184038
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DOI: https://doi.org/10.1007/BF00184038