Summary
An important molecular approach for the study of the complex processes implicated in prostatic carcinogenesis is the determination of steady-state mRNA levels of potentially relevant genes. One important category of these genes are the proto-oncogenes. Previous studies on the involvement of oncogenes in prostatic carcinogenesis, using primary human prostatic tumors, have shown that the ras and myc oncogenes might be related to the progression of this disease. We tested this hypothesis in the Dunning R-3327 rat prostatic cancer model system, which consists of a series of sublines that represent the different stages of tumor progression in prostatic cancer. We could not show a correlation between the expression of the ras and myc proto-oncogenes and the state of progression of the disease. The levels and patterns of expression of other proto-oncogenes, however, might be more useful as markers for malignancy and/or progression of prostatic cancer in rats.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Auffray C, Rougeon F (1980) Purification of mouse immunoglobulin heavy chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem 107:303–314
Barbacid M (1987) ras Genes. Annu Rev Biochem 56:779–827
Ben-Neriah Y, Bernards A, Paskind M, Daley GQ, Baltimore D (1986) Alternative 5′ exons in c-abl mRNA. Cell 44:577–586
Bishop JM (1987) Trends in oncogenes. In: Bradshaw RA, Prentis S (eds) Oncogenes and Growth Factors. Elsevier, Amsterdam, pp 1–10
Bonner TI, Opperman H, Seeburg P, Kerby SB, Gunnell MA, Young AC, Rapp UR (1986) The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 gene. Nucleic Acids Res 14:1009–1015
Bussemakers MJG, Van de Ven WJM, Debruyne FMJ, Schalken JA (1991) Identification of high mobility group protein I(Y) as a potential marker for prostate cancer by differential hybridization analysis. Cancer Res 51:606–611
Buttyan R, Sawczuk IS, Benson MC, Siegal JD, Olsson CA (1987) Enhanced expression of the c-myc proto-oncogene in high grade human prostate cancers. Prostate 11:327–337
Campisi J, Gray HE, Pardee AB, Dean M, Sonenshein GE (1984) Cell-cycle control of c-myc but not c-ras expression is lost following chemical transformation. Cell 36:241–247
Cooke DB, Quarmby VE, Mickey DD, Isaacs JT, French FS (1988) Oncogene expression in prostate cancer: Dunning R-3327 rat dorsal prostatic adenocarcinoma system. Prostate 13:263–272
Cooke DB, Quarmby VE, Petrusz P, Mickey DD, Der CJ, Isaacs JT, French FS (1988) Expression of ras proto-oncogenes in the Dunning R-3327 rat prostatic adenocarcinoma system. Prostate 13:273–288
Fleming WH, Hamel A, MacDonald R, Ramsey E, Pettigrew NM, Johnston B, Dodd JG, Matusik RJ (1986) Expression of the c-myc proto-oncogene in human prostatic carcinoma and benign prostatic hyperplasia. Cancer Res 46:1535–1538
Hinds P, Finla C, Levine AJ (1989) Mutation is required to activate the p53 gene for cooperation with the ras oncogene and transformation. J Virol 63:739–746
Isaacs JT, Hukku B (1988) Nonrandom involvement of chromosome 4 in the progression of rat prostatic cancer. Prostate 13:165–188
Isaacs JT, Isaacs WB, Feitz WFJ, Scheres J (1986) Establishment and characterization of seven Dunning rat prostatic cancer cell lines and their use in developing methods for predicting metastatic abilities of prostatic cancers. Prostate 9:261–281
Meijer D, Hermans A, Von Lindern M, Van Agthoven T, De Klein A, Mackenbach P, Grootegoed A, Talarico D, Della Valle G, Grosveld G (1987) Molecular characterization of the testis specific c-abl mRNA in mouse. EMBO J 6:4041–4048
Muller E, Slamon DJ, Tremblay JM, Cline MJ, Verma IM (1982) Differential expression of cellular oncogenes during pre- and post-natal development of the mouse. Nature 299:640–644
Peehl DM, Wehner N, Stamey TA (1987) Activated Ki-ras oncogene in human prostatic adenocarcinoma. Prostate 10:281–289
Rijnders AWM, Van der Korput JAGM, Van Steenbrugge GJ, Romijn JC, Trapman J (1985) Expression of cellular oncogenes in human prostatic carcinoma cell lines. Biochem Biophys Res Commun 132:548–554
Viola MV, Fromowitz F, Oravez S, Deb S, Finkel G, Lundy J, Hand P, Thor A, Schlom J (1986) Expression of ras oncogene p21 in prostate cancer. N Engl J Med 314:133–137
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bussemakers, M.J.G., Isaacs, J.T., Debruyne, F.M.J. et al. Oncogene expression in prostate cancer. World J Urol 9, 58–63 (1991). https://doi.org/10.1007/BF00184034
Issue Date:
DOI: https://doi.org/10.1007/BF00184034