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Superinducers for induction of thermostable lipase production by Pseudomonas species NT-163 and other Pseudomonas-like bacteria

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Biotechnology Techniques

Summary

Among various additives that affected production of a new thermostable extracellular lipase from Pseudomonas sp. NT-163 , stearyl alcohol was the most effective. Addition of stearyl alcohol (0.5%) brought about ca. 500 fold enhancement of the lipase activity (200 U/ml by p-nitorophenyllaurate method) compared to the case with no additive (≤ 0.4 U/ml), while olive oil attained only 12–15 U/ml. Palmityl and oleyl alcohols also were highly effective as lipase inducers (150–160 U/ml could be attained). Furthermore, stearyl alcohol induced lipase formation in several other Bacteriol strains 10-times more than olive oil.

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Authors and Affiliations

  1. Department of Industrial Chemistry, Niihama National College of Technology, Yakumo 7-1, Niihama, 792, Ehime

    Kazutoshi Ushio, Takuya Hirata, Katsutoshi Yoshida, Miho Sakaue, Chizuru Hirose & Toshiki Suzuki

  2. Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Kasuga, Bunkyo, 112, Tokyo, Japan

    Morio Ishizuka

Authors
  1. Kazutoshi Ushio
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  2. Takuya Hirata
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  3. Katsutoshi Yoshida
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  4. Miho Sakaue
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  5. Chizuru Hirose
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  6. Toshiki Suzuki
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  7. Morio Ishizuka
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Ushio, K., Hirata, T., Yoshida, K. et al. Superinducers for induction of thermostable lipase production by Pseudomonas species NT-163 and other Pseudomonas-like bacteria. Biotechnol Tech 10, 267–272 (1996). https://doi.org/10.1007/BF00184027

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  • DOI: https://doi.org/10.1007/BF00184027

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