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Novel method using antibiotics for the determination of the rate-limiting step in the secretion pathway of glucoamylase from recombinant yeast

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Biotechnology Techniques

Summary

A novel method for the determination of the rate-limiting step in the protein secretion pathway in recombinant strains of Saccharomyces cerevisiae is proposed which used several antibiotics that specifically inhibit the processing enzymes in the secretion pathway. This novel method has advantages such as the relative simplicity of the experiments, non-requirement of secretion mutants, and absence of strain effects.

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References

  • Carlson, M. and Botstein, D. (1982). Cell. 28, 145–154.

    Google Scholar 

  • Cha, H.J., Yoo, Y.J., Ahn, J.H., and Kang, H.S. (1992). Biotech. Lett. 14, 747–752.

    Google Scholar 

  • Cha, H.J., Cho, K.M. and Yoo, Y.J. (1994). Kor. J. Biotechnol. Bioeng. 9, 532–540.

    Google Scholar 

  • Duronio, V., Jacobs, S., Romero, P.A., and Herscovics, A. (1988). J. Biol. Chem. 263, 5436–5445.

    Google Scholar 

  • Elliott, S., Giffin, J., Suggs, S., Lau, E.P., and Banks, A.R. (1989). Gene. 79, 167–180.

    Google Scholar 

  • Greber, U.F., Sode, K., and Meussdoerffer, F. (1988). Enzyme Microb. Technol. 10, 246–251.

    Google Scholar 

  • Hurn, B.A.L. and Chantler, S.M. (1980). Methods Enzymol. 70, 104–142.

    Google Scholar 

  • Kingsman, S.M., Kingsman, A.J., and Mellor, J. (1987). Trends Biotechnol. 5, 53–57.

    Google Scholar 

  • Kovaleva, I.E., Novikova, L.A., and Luzikov, V.N. (1989). FEBS-Lett. 251, 183–186.

    Google Scholar 

  • Lodish, H.F. and Kong, N. (1984). J. Cell. Biol. 98, 1720–1729.

    Google Scholar 

  • Lodish, H.F. (1988). J. Biol. Chem. 263, 2107–2110.

    Google Scholar 

  • Moir, D.T. and Dumais, D.R. (1987). Gene. 56, 209–217.

    Google Scholar 

  • Morosoli, R., Lecher, P., and Durand, S. (1988). Arch. Biochem. Biophys. 265, 183–189.

    Google Scholar 

  • Park, S.J. and Ramirez, W.F. (1989). Biotech. Bioeng. 33, 272–281.

    Google Scholar 

  • Saunier, B., Kilker, R.K., Tkacz, J.S., and Herscovics, A. (1982). J. Biol. Chem. 257, 14155–14161.

    Google Scholar 

  • Schauer, I., Emr, S., Gross, C., and Schekman, R. (1985). J. Cell Biol. 100, 1664–1675.

    Google Scholar 

  • Yamashita, I., Suzuki, K., and Fukui, S. (1985). J. Bacteriol. 161, 567–573.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering and The Institute for Molecular Biology and Genetics, Seoul National University, 151-742, Seoul, Korea

    Hyung Joon Cha & Young Je Yoo

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  1. Hyung Joon Cha
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  2. Young Je Yoo
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Cha, H.J., Yoo, Y.J. Novel method using antibiotics for the determination of the rate-limiting step in the secretion pathway of glucoamylase from recombinant yeast. Biotechnol Tech 10, 257–262 (1996). https://doi.org/10.1007/BF00184025

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  • DOI: https://doi.org/10.1007/BF00184025

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