Biotechnology Techniques

, Volume 10, Issue 4, pp 253–256 | Cite as

Continuous separation of proteins by poly(vinyl alcohol) shielded Multichannel Flow Electrophoresis

  • Zheng Liu
  • Jin Wang
  • Zheng Huang
  • Fuxin Ding
  • Zhongyao Shen
  • Naiju Yuan
Article

Summary

A hydrophilic polysulphone membrane spaced multicompartment electrolyzer for Multichannel Flow Electrophoresis (MFE) was developed. Application of poly(vinyl alcohol) (PVA) in MFE resulted in a 35% increase of protein transmembrane flux. Continuous separation of bovine serum albumin (BSA) and haemoglobin bovine blood (HBB) model mixture by PVA shielded MFE yielded 56mg BSA and 48mg HBB per hour. The average recovery was 65%.

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References

  1. Grag, N., Galev, I.Y. and Mattiasson, B.(1994), Biotechnology Techniques, 8, 645–50Google Scholar
  2. Liu, Z., Huang, Z., Cong, J.-Y., Ding, F.-X., Yuan, N.-J. (1995), Sep. Sci. & Tech. In press.Google Scholar
  3. Liu, Z., Yang H., Huang, Z., Ding, F.-X., Yuan, N.-J. (1995), Submitted to Sep. Sci. & Tech. Google Scholar
  4. Yuan, N.-J., Liu, Z., Zhu, D.-Q. and Ding, F.-X. (1993), Applied for China Patent, Appl. No. 93108630.2–4.Google Scholar

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Zheng Liu
    • 1
  • Jin Wang
    • 1
  • Zheng Huang
    • 1
  • Fuxin Ding
    • 1
  • Zhongyao Shen
    • 1
  • Naiju Yuan
    • 1
  1. 1.Dept. of Chemical EngineeringTsinghua UniversityBeijingPeople's Republic China

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