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Comparison of the effects of prostaglandins E2 and I2 on testicular nociceptor activities studied in vitro

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Summary

Effects of exogenous prostaglandin (PG) E2 and PGI2 on testicular polymodal receptor activities were compared in in vitro recordings of single- or multi-fiber discharges from canine testis-spermatic nerve preparations. PGI2 up to 1.4×10−6 mol/l (cumulative method) or 1.0×10−5 mol/l (non-cumulative method) excited only weakly some of the receptors, and similar observations were made with PGE2. Both PGs applied cumulatively or non-cumulatively at concentrations above 1.4×10−8 mol/l augmented the response to bradykinin (9.4×10−8 mol/l) in more than half of the cases tested. The augmenting effect of PGE2 lasted longer than that of PGI2 both with the cumulative and the non-cumulative method. The degree of augmentation tended to increase dependent on concentration, but some cases showed no further increase or rather a decrease in augmentation by PGs at a ten times higher concentration, especially when PGs were applied cumulatively. A second challenge by PG after a short interval (2 min) did not induce augmentation. These phenomena were considered to be tachyphylaxis to PGs. Cross-tachyphylaxis to PGE2 and PGI2 was also observed. There was not much difference in excitatory and augmenting potencies between these two PGs, but there was a clear difference in the concentrations of the PGs necessary to induce excitation of polymodal receptors and to facilitate their response to bradykinin.

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Authors and Affiliations

  1. Department of Nervous and Sensory Functions, Research Institute of Environmental Medicine, Nagoya University, 464-01, Nagoya, Japan

    K. Mizumura, J. Sato & T. Kumazawa

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  1. K. Mizumura
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  2. J. Sato
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  3. T. Kumazawa
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Mizumura, K., Sato, J. & Kumazawa, T. Comparison of the effects of prostaglandins E2 and I2 on testicular nociceptor activities studied in vitro. Naunyn-Schmiedeberg's Arch Pharmacol 344, 368–376 (1991). https://doi.org/10.1007/BF00183013

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  • DOI: https://doi.org/10.1007/BF00183013

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