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Glycine-evoked release of [3H]acetylcholine from rat striatal slices is independent of the NMDA receptor

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Summary

KCl-, NMDA-, and glycine-evoked release of [3H]acetylcholine was studied in superfused rat striatal slices. KCl-evoked release of [3H]acetylcholine was inhibited by 1.2 mM MgC12 and 100 μM lidocaine. Similarly, NMDA-evoked release was inhibited by MgCl2 and lidocaine as well as 10 μM CGS 19755, a competitive antagonist at NMDA receptors, and 10 nM MK-801, a noncompetitive antagonist of NMDA-induced responses. Glycine-evoked release was calcium-dependent and was inhibited by 0.1 μM strychnine whereas KCl- and NMDA-evoked release were resistant to strychnine. In addition, lidocaine inhibited the glycine-induced response. Cross-tachyphylaxis was not observed between NMDA- and glycine-evoked release. These results indicate that the strychnine-sensitive, glycine-evoked release of [3H]acetylcholine is independent of the NMDA receptor.

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Authors and Affiliations

  1. Ciba-Geigy Corp., Neuroscience Research, Pharmaceuticals Division, 07901, Summit, NJ, USA

    Cynthia A. Taylor, Cindy Tsai & John Lehmann

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  1. Cynthia A. Taylor
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  2. Cindy Tsai
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  3. John Lehmann
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Taylor, C.A., Tsai, C. & Lehmann, J. Glycine-evoked release of [3H]acetylcholine from rat striatal slices is independent of the NMDA receptor. Naunyn-Schmiedeberg's Arch Pharmacol 337, 552–555 (1988). https://doi.org/10.1007/BF00182730

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  • DOI: https://doi.org/10.1007/BF00182730

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