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Serum-induced collagen gel contraction

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Abstract

• Purpose: To understand the molecular events underlying disease-related vitreous gel contraction, the effect of serum components on collagen was investigated. • Methods: Bovine vitreous or dermal collagen was incubated with a mixture of transglutaminase (TG; factor XIIIa) and fibronectin (FN), and the biochemical changes of collagen were monitored by gel electrophoresis. In addition, serum-induced changes in the volume of the collagen gel were monitored. • Results: Gel electrophoresis revealed a new high-molecular-weight band (Mr 240 000) presumably due to intermolecular cross-links of collagen peptides and FN. The serum components also were shown to cause a significant decrease in the volume of the collagen gel. • Conclusion. Collagen gel contraction could be attributed to the collagen-FN-collagen cross-links catalyzed by TG.

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Authors and Affiliations

  1. Department of Ophthalmology, Harvard Medical School, Schepens Eye Research Institute, Boston, Massachusetts, USA

    Jun Akiba, Akihiro Kakehashi, Norio Ueno & Bireswar Chakrabarti

  2. Department of Ophthalmology, Asahikawa Medical College, 4-5 Nishikagura, 078, Asahikawa, Japan

    Jun Akiba & Akihiro Kakehashi

  3. Department of Ophthalmology, Osaka University Medical School, Osaka, Japan

    Norio Ueno & Yasuo Tano

Authors
  1. Jun Akiba
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  2. Akihiro Kakehashi
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  3. Norio Ueno
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  4. Yasuo Tano
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  5. Bireswar Chakrabarti
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Akiba, J., Kakehashi, A., Ueno, N. et al. Serum-induced collagen gel contraction. Graefe's Arch Clin Exp Ophthalmol 233, 430–434 (1995). https://doi.org/10.1007/BF00180947

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  • DOI: https://doi.org/10.1007/BF00180947

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