Abstract
• Background: In a previous paper, we reported that retinal blood flow (RBF) ceased immediately after injection of 1 nmol endothelin-1 (ET-1) and no recovery of RBF was detected for at least 50 min. In this study, we confirmed the same duration of RBF cessation and measured choroidal blood flow (CBF) for 180 min. • Methods: We measured CBF in a rabbit model of transient complete obstruction of retinal vessels induced by intravitreal injection of a high dose of ET-1, using the hydrogen clearance method. We also investigated the effects of intravitreal injection of ET-1 on intraocular pressure (IOP), blood pressure, pulse rate and blood gases. • Results: CBF was significantly greater in the ET-1-injected eyes than in the control eyes 40–130 min after injection of ET-1 (P < 0.05). The maximal CBF ratio in the ET-1-injected eyes was 128 ± 7.4% at 40 min. CBF decreased to the pre-injection level at 140 min after the injection of ET-1. There was no significant change in blood pressure, pulse rate and blood gases throughout this experiment, and there was no significant difference in IOP between ET-1-injected eyes and control eyes. • Conclusion: It seems likely that the increase in CBF resulted from some local mechanisms of control that compensated for the decrease in RBF induced by intravitreal injection of ET-1. This model may be useful for investigation of the regulatory system of intraocular circulation, including endothelin receptors.
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References
Alm A, Bill A (1972) The oxygen supply to the retina. II. Effect of high intraocular pressure and of increased arterial carbon dioxide tension on uveal and retinal blood flow in cats. Acta Physiol Scand 84:306–319
Alm A, Bill A (1987) Ocular circulation. In: Moses RA, Hart WM, Jr. (eds) Adler's physiology of the eye: clinical application. Mosby, St Louis, pp 183–203
Asano T, Ikegaki I, Suzuki Y, Satoh S, Shibuya M (1989) Endothelin and the production of cerebral vasospasm in dogs. Biochem Biophys Res Commun 159:1345–1351
Bill A (1984) Circulation in the eye. In: Renkin EM, Michel CC (eds) Handbook of physiology: the cardiovascular system. Waverly Press, Baltimore, pp 1001–1034
Friedman E (1970) Choroidal blood flow pressure relationships. Arch Ophthalmol 83:95–99
Friedman E, Chandra S (1972) Choroidal blood flow, effects of oxygen and carbon dioxide. Arch Ophthalmol 87:70–71
Granger HJ, Shepherd AP (1973) Intrinsic microvascular control of tissue oxygen delivery. Microvase Res 5:49–72
Hyvärinen L (1967) Vascular structures of the rabbit retina. Acta Ophthalmol (Copenh) 45:852–861
Kiel J, Shepherd A (1992) Autoregulation of choroidal blood flow in the rabbit. Invest Ophthalmol Vis Sci 33:2399–2410
Kimura Y, Nitta A, Shimizu R, Takayama H, Aoki K (1988) In vivo measurement of blood flow volume in the monkey rertina. Jpn J Ophthalmol 92:71–78
Kraus GE, Bucholz RD, Yoon KW, Knuepfer MM, Smith KR (1991) Cerebrospinal fluid endothelin-1 and endothelin-3 levels in normal and neurosurgical patients: a clinical study and literature review. Surg Neurol 35:20–29
Kurihara H, Yoshizumi M, Sugiyama T, Yamaoki K, Nagai R, Takaku F, Sato H, Inui J, Yanagisawa M, Masaki T, Yazaki Y (1989) The possible role of endothelin-1 in the pathogenesis of coronary vasospasm. J Cardiovasc Pharmacol 13: S132-S142
MacCumber MW, Jampel HD, Snyder SH (1991) Ocular effect of the endothelins. Arch Ophthalmol 109:705–709
Masaki T, Kimura S, Yanagisawa M, Goto K (1991) Molecular and cellular mechanism of endothelin regulation. Implication for vascular function. Circulation 84:1457–1468
Miyauchi T, Yanagisawa M, Tomizawa T; Sugishita Y, Suzuki N, Fujino M, Ajisaka R, Goto K, Masaki T (1989) Increased plasma concentrations of endothelin-1 and big endothelin-1 in acute myocardial infarction. Lancet 2:53–54
Riva CE, Grunwald JE, Petrig BL (1986) Autoregulation of human retinal blood flow: an investigation with laser doppler velocity. Invest Ophthalmol Vis Sci 27:1706–1712
Sato T, Takei K, Nonoyama T, Miyauchi T, Goto K, Hommura S (1993) Endothelin-1-induced vasoconstriction on retinal vessels in rabbit. Jpn J Ophthalmol 97:683–689
Stefansson E, Wagner HG, Seida M (1988) Retinal blood flow and its autoregulation measured by intraocular hydrogen clearance. Exp Eye Res 47:669–678
Takahashi K, Brooks RA, Kanse SM, Ghati MA, Kohner EM, Bloom SR (1989) Production of endothelin-1 by cultured bovine retinal endothelial cells and presence of endothelin receptors on associated pericytes. Diabetes 38:1200–1202
Takahashi S (1981) Studies on choroidal blood flow using the hydrogen clearance method. 1. The normal eye in the rabbit and monkey. Ophthalmol Jap 32:510–519
Takei K, Sato T, Nonoyama T, Miyauchi T, Goto K; Hommura S (1993) A new model of transient complete obstruction of retinal vessels by endothelin-1 injection into the posterior vitreous body in rabbits. Graefe's Arch Clin Exp Ophthalmol 231:476–481
Takei K, Sato T, Nonoyama T, Hommura S, Miyauchi T, Goto K (1993) Analysis of vasocontractile responses to endothelin-1 in rabbit retinal vessels using an ETA receptor antagonist and an ETB receptor agonist. Life Sci 53: LP111–115
Tomobe Y, Ishikawa T, Yanagisawa M, Kimura S, Masaki T, Goto K (1991) Mechanisms of altered sensitivity to endothelin-1 between aortic smooth muscles of spontaneously hypertensive and Wistar-Kyoto rats. J Pharmacol Exp Ther 257:555–561
Weiter JJ, Schachar RA, Ernest JT (1973) Control of intraocular blood flow. I. Intraocular pressure. Invest Ophthalmol 12:327–331
Yanagisawa M, Masaki T (1989) Molecular biology and biochemistry of endothelins. Trends Pharmacol Sci 10:374–378
Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K, Masaki T (1988) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332:411–415
Yu DY, Alder VA, Cringle SJ (1988) Choroidal blood flow measured in the dog eye in vivo and in vitro by local hydrogen clearance polarography: validation of a technique and response to raised intraocular pressure. Exp Eye Res 46:289–303
Yu DY, Alder VA, Cringle SJ (1990) The validity of hydrogen clearance measurements of retinal blood flow. Exp Eye Res 50:533–539
Yu DY, Alder VA, Cringle SJ (1991) Measurement of blood flow in rat eyes by hydrogen clearance. Am J Physiol 261:H960-H968
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Sato, T., Takei, K., Nonoyama, T. et al. Increase in choroidal blood flow in rabbits with endothelin-1 induced transient complete obstruction of retinal vessels. Graefe's Arch Clin Exp Ophthalmol 233, 425–429 (1995). https://doi.org/10.1007/BF00180946
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DOI: https://doi.org/10.1007/BF00180946