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Genetic and environmental effects on in vitro shoot regeneration from cotyledon explants of Brassica juncea

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Abstract

Twenty-one lines of Brassica juncea were screened for frequency of in vitro shoot formation from cultured cotyledons of 8-day old seedlings. All brown-seeded Indian lines showed good shoot regeneration with 20–50% of cotyledons giving rise to shoots. Very poor shoot regeneration (0–12%) was observed for the predominantly yellow-seeded Chinese/European lines, including two erucic acid-free lines. Germination of seedlings in hydroponic nutrient solution markedly enhanced subsequent shoot regeneration frequency from cotyledons of three Indian lines but had no effect on shoot formation from the recalcitrant Chinese/European lines.

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Authors and Affiliations

  1. Biotechnology Unit, State Chemistry Laboratory, 5 Macarthur Street, 3002, East Melbourne, Victoria, Australia

    G. A. Fazekas, P. A. Sedmach & M. V. Palmer

Authors
  1. G. A. Fazekas
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  2. P. A. Sedmach
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  3. M. V. Palmer
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Fazekas, G.A., Sedmach, P.A. & Palmer, M.V. Genetic and environmental effects on in vitro shoot regeneration from cotyledon explants of Brassica juncea . Plant Cell Tiss Organ Cult 6, 177–180 (1986). https://doi.org/10.1007/BF00180802

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  • DOI: https://doi.org/10.1007/BF00180802

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