Abstract
Protoplasts of Cyamopsis tetragonoloba were isolated from leaves of in vitro grown plants. The yield of the protoplasts, their viability and subsequent divisions were greatly influenced by the pH of the media used for isolation and culture of protoplasts. Sustained divisions of the cultured protoplasts were best supported by a modified Kao and Michayluk (1975) nutrient medium containing glucose (0.4 M), NAA (4 mgl−1), 2,4-D (1 mgl−1) and KIN (2 mgl−1 ). The protoplast derived cells developed calli on transfer to Murashige and Skoog (1962) medium supplemented with 1 mgl−1 each of 2,4-D, NAA and KIN.
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Saxena, P.K., Gill, R. & Rashid, A. Isolation and culture of protoplasts from mesophyll tissue of the legume Cyamopsis tetragonoloba L.. Plant Cell Tiss Organ Cult 6, 173–176 (1986). https://doi.org/10.1007/BF00180801
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DOI: https://doi.org/10.1007/BF00180801