Abstract
In vitro multiplication of banana (Musa spp.) from shoot-tip explants isolated from lateral suckers is described. Using explants with apical domes, a total of 22 banana cultivars were successfully cultured on a modified Murashige and Skoog's medium containing 6-benzylaminopurine (BA) and indolebutyric acid (IBA). Shoot-tip explants could be induced to produce multiple shoot initials in the presense or absence of apical domes, but the survival rates were higher if apical domes were retained. Cultivars varied widely in their multiplication rates in response to cytokinins, BA being consistently more effective than kinetin (Kn). Although Kn was less effective in this regard, it stimulated vigorous root growth. Rooted plantlets were successfully established in soil.
This is a preview of subscription content, log in via an institution to check access.
References
Bower JP, Fraser C (1982) Shoot tip culture of Williams bananas. Subtropica 3:13–16
Cronauer SS, Krikorian AD (1984) Rapid multiplication of bananas and plantains by in vitro shoot tip culture. Hort Science 19:234–235
De Guzman EV, Decena AC, Ubalde EM (1980) Plantlet regeneration from unitradiated and irradiated banana shoot tip tissues cultured in vitro. Phil Agri 63:140–146
Hwang SC, Chen CL, Lin JC, Lin HL (1984) Cultivation of banana using plantlets from meristem culture. Hort Science 19:231–233
Hu CY, Wang PJ (1983) Meristem, shoot tip and bud cultures. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture. Volume 1. Techniques for propagation and breeding. MacMillan Publishing Co: New York and London, pp 177–227
Jarret RL, Rodriguez W, Fernandez R (1985) Evaluation, tissue culture propagation, and dissemination of ‘SABA’ and ‘PELIPITA’ plantains in Costa Rica. Sci Hort 25:137–147
Ma SS, Shii CT (1972) In vitro formation of adventitious buds in banana shoot apex following decapitation. J Hort Soc China 18:135–142
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Phillips IDJ (1975) Apical dominance. Ann Rev Plant Physiol 26:341–367
Simmonds NW (1966) Banana, 2nd edition. Longmans: London, 512 pp
Skutch AF (1932) Anatomy of the axis of the banana. Bot Gaz 93:233–258
Stover RH (1972) Banana, plantain and abaca diseases. Commw Mycol Inst, Kew, UK, 316 pp
Swamy RD, Rao NKS, Chacko EK (1983) Tissue-culture propagation of banana. Sci Hort 18:247–252
Vessey JC, Rivera JA (1981) Meristem culture of bananas. Turrialba 31:162–163
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wong, W.C. In vitro propagation of banana (Musa spp.): initiation, proliferation and development of shoot-tip cultures on defined media. Plant Cell Tiss Organ Cult 6, 159–166 (1986). https://doi.org/10.1007/BF00180799
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00180799