Summary
Changes in distribution of elastin and elastin receptor were studied during maturation of the ductus arteriosus. In this vessel intimal thickening is a normal process. It starts with separation of endothelial cells from the internal elastic lamina by the formation of a wide subendothelial region, followed by an increase in number of radially orientated cells in the inner media and subendothelial region. For the first time the nature of these originally inner media cells could be definitely determined as being derived from smooth muscle cells. Areas rich in these modified smooth muscle cells contained less elastin as compared with regions rich in circularly orientated smooth muscle cells. The internal elastic lamina had disappeared underneath intimal cushions of the canine ductus arteriosus, whereas in the fetal human ductus arteriosus, splitting of the internal elastic lamina was observed. Elastin was not present underneath separated endothelial cells, which suggests that these cells do not synthesize elastin, at least not after separation. Both smooth muscle cells and endothelial cells demonstrated the presence of the elastin receptor. Changes in its distribution were not observed. The temporal and spatial association between the altering distribution of elastin and the absence of normal cushion formation in the persistent ductus arteriosus suggests a role of the elastin receptor in the process of intimal thickening.
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References
Buchanan JW (1978) Morphology of the abnormal ductus arteriosus in dogs. In: Heymann MA, Rudolph AM (eds) The ductus arteriosus. Report of the 75th Ross conference on pediatric research. Ohio: Ross Laboratories, pp 9–15
Chuaqui BJ, Piwonka GC, Farr OA (1977) Uber den Wandbau des persisterenden Ductus arteriosus. Virchow Arch [A] 372:315–324
De Reeder EG, Girard N, Poelmann RE, van Munsteren JC, Patterson DF, Gittenberger-de Groot AC (1988) Hyaluronic acid accumulation and endothelial separation in intimal thickening of the vessel wall: the normal and genetic defective ductus arteriosus. Am J Pathol 132:574
Gittenberger-de Groot AC (1977) Persistent ductus arteriosus: most probably a primary congenital malformation. Br Heart J 6:610–618
Gittenberger-de Groot AC, Strengers AC, Mentink M, Poelmann RE, Patterson DF (1985) Histological studies on normal and persistent ductus arteriosus of the dog. Am J Cardiol 6:393–404
Grande JG, Davis HR, Bates S, Mathews MB, Glasgov S (1987) Effect of an elastin growth substrate on cholesterol ester synthesis and foam cell formation by cultured aortic smooth muscle cells. Atherosclerosis 68:87–93, 184–195
Guide to the care and use of laboratory animals (1985) PHA NIH Publ, No 85-23, revised
Hinek A, Wrenn DS, Mecham RP, Barondes SH (1988) The elastin receptor: a galactoside-binding protein. Science 239:1539–1541
Jacob MP, Fulop T, Foris G, Robert L (1987) Effect of elastin peptides on ion fluxes in mononuclear cells, fibroblasts, and smooth muscle cells. Proc Natl Acad Sci USA 84:995–999
Mecham RP, Griffin GL, Madaras JC, Senior RM (1984) Appearance of chemotactic responsiveness to elastin peptides by developing fetal bovine ligament fibroblasts parallels the onset of elastin production. J Cell Biol 98:1813–1816
Nakatake J, Yamamoto T (1987) Three-dimensional architecture of elastic tissue in atheroarteriosclerotic lesions of the rat aorta. Atherosclerosis 64:191–200
Nakatake J, Wasano K, Yamamoto T (1985) Three-dimensional architecture of elastic tissue in early atherosclerotic lesions of the rat aorta. Atherosclerosis 57:199–208
Patterson DF, Pyle RL, Buchanan JW, Trautvetter E, Abt DA (1971) Hereditary patent ductus arteriosus and its sequelae in the dog. Circ Res 29:1–13
Rabinovitch M, Beharry S, Bothwell T, Jackowski G (1988) Qualitative and quantitative differences in protein synthesis comparing fetal lamb ductus arteriosus endothelium and smooth muscle with cells from adjacent vascular sites. Dev Biol 130:250–258
Strengers JLM (1988) Fetal and postnatal behavior of the ductus ateriosus. Thesis; Leiden
Strengers JLM, Poelmann RE, Patterson DF, Harinck E, Gittenberger-de Groot AC (1986) Comparison of light and electron microscopic studies of the normal and persistent ductus arteriosus in human and dogs. In: Doyle EF, Engle MA (eds) Pediatric Cardiology. Proceedings of the 2nd World Congress. Springer, New York, pp 868–870
Toda T, Tsuda N, Takagi T, Nishimori I, Leszczynski D, Kummerow F (1980) Ultrastructure of developing human ductus arteriosus. J Anat 131:25–37
Todorovitch-Hunter L, Johnson DJ, Ranger P, Keeley FW, Rabinovitch M (1988) Altered elastin and collagen synthesis associated with progressive pulmonary hypertension induced by monocrotaline. Lab Invest 58:184–195
Tsukada T, Tippens D, Gordon D, Ross R, Gown MA (1987) HHF35, A muscle-actin-specific monoclonal antibody. Am J Pathol 126:51–60
Wrenn DS, Hinek A, Mecham RP (1988) Kinetics of receptor-mediated binding of tropo-elastin to ligament fibroblasts. J Biol Chem 263:2280–2284
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This study is supported by the Dutch Heart Foundation (grant 85103) and National Institutes of Health (grant HL 18848). Part of the results have been published in a monograph by the first author; title: Maturation of the ductus arteriosus; a model for intimal thickening
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de Reeder, E.G., van Munsteren, C.J., Poelmann, R.E. et al. Changes in distribution of elastin and elastin receptor during intimal cushion formation in the ductus arteriosus. Anat Embryol 182, 473–480 (1990). https://doi.org/10.1007/BF00178912
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DOI: https://doi.org/10.1007/BF00178912