Abstract
The anti-HLA-DQ3 monoclonal antibodies (mAb) KS13, SO1, SO2, SO3, SO4, and SO5 recognize spatially close but distinct antigenic determinants, since they crossinhibit each other in their binding to HLA-DQ3 antigens, but do not share idiotopes recognized in their antigen combining site by syngeneic and anti-id antisera and mAb. Furthermore, mAb SO1, SO3, SO4, and SO5 react also with HLA-DQ allospecificities other than HLA-DQ3. Sequence analysis of the heavy (V H ) and light (V L ) chain variable region of the six mAb revealed preferential usage of V H 36–60 and V K 12/13 gene families. However, the individual V H and V L germline gene usage by the six mAb is diverse and the utilization of D, J H , and J L gene segments is heterogeneous. The diverse usage of V H and V L gene segments and heterogeneous amino acid sequences of V H and V L CDR, together with the heterogeneous idiotypic profile, may reflect the complexity of the determinants recognized by the six mAb on HLA-DQ3 antigens. The results we have presented provide for the first time information about the structural basis of the diversity of antibodies recognizing human histocompatibility antigens.
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The nucleotide sequence data reported in this Papershave been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers L20499, L20957, L20961, L24557, L24558 and L20962, respectively, for V H region genes, and L20956, L20958, L24555, L24556, L20959, and L20960, respectively, for V L region genes
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Iwasaki, Y., Takabatake, H., Monestier, M. et al. Idiotypic diversity and variable region gene usage by mouse anti-HLA-DQ3 mAb. Immunogenetics 42, 90–100 (1995). https://doi.org/10.1007/BF00178583
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DOI: https://doi.org/10.1007/BF00178583