Summary
Regulatory peptide immunoreactivities reported in the upper respiratory system of man include vasoactive intestinal polypeptide (VIP) and peptide histidine methionine (PHM), which are co-localized in a network of fine varicose nerve fibers. The present study was undertaken to examine the possible occurrence and distribution of the recently described VIP-like peptide helospectin. Double immunofluorescence labelling showed that helospectin is co-localized with VIP and PHM. In addition to nerve fibers containing all three peptides, scattered nerve fibers were detected that were only immunoreactive to helospectin but not to VIP and/or PHM. The distribution of Heospectin/VIP/PHM immunoreactive nerve fibers around blood vessels and in close relationship to seromucous glands indicates their possible involvement in the regulation of blood flow and secretion.
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Absood A, Ekblad E, Ekelund M, Hakanson R, Sundler F (1992) Helospectin-like peptides in the gastrointestinal tract. Immunocytochemical localization and immunochemical characterization. Neuroscience 46:431–438
Albegger K, Hauser-Kronberger CE, Saria A, Graf A-H, Bernatzky G, Hacker GW (1991) Regulatory peptides and general neuroendocrine markers in the human nasal mucosa, the soft palate and the larynx. Acta Otalaryngol (Stockh) 111: 373–378
Coons AH, Leduc EH, Connolly JM (1955) Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med 102:49–60
Dahlquist A, Forsgren S (1989) Networks of peptide-containing nerve fibers in laryngeal nerve paraganglia. Acta Otolaryngol(Stockh) 107:289–295
Diamond L, Szarek JL, Gillespie MN (1983) In vivo bronchodilator activity of vasoactive intestinal peptide in the cat. Am Rev Respir Dis 128:827–832
Ekstroem J (1987) Neuropeptides and secretion. J Dent Res 66:524–530
Goyal RK, Rattan S, Said SI (1980) VIP as a possible neurotransmitter of noncholinergic nonadrenergic inhibitory neurons. Nature 288:378–380
Grundemar L, Hogestatt ED (1990) Vascular effects of helodermin, helospectin I and helospectin II: a comparison with vasoactive intestinal peptide (VIP) Br J Pharmacol 99:526–528
Hauser-Kronberger CE, Hacker GW, Saria A, Graf A-W, Albegger K (1991) Regulatorische Peptide und generelle neuroendokrine Marker im oberen Respirationstrakt des Menschen. Allergologie 6:224–230
Huang WM, Gibson SJ, Facer P, Gu J, Polak JM (1983) Improved section adhesion for immunocytochemsitry using high molecular weight polymers of L-lysine as a slide coating. Histochemistry 77:275–279
Ichimura K, Mineda H, Seki A (1988) Vascular effects of neuropeptides on nasal mucosa. Ann Otol Rhinol Laryngol 97: 289–293
Lacroix JS (1989) Adrenergic and non-adrenergic mechanisms in sympathetic vascular control of the nasal mucosa. Acta Physiol Scand 136 [Suppl 581]: 1–63
Lindh B, Lundberg JM, Hokfelt T (1989) NPY-, galanin-, VIP/PHI-, CGRP- and substance P-immunoreactive neuronal subpopulations in cat autonomic and sensory ganglia and their projections. Cell Tissue Res 256:259–273
Lundberg JM, Fahrenkrug J, Hökfelt T, Martling CR, Larsson O, Tatemoto K, Angard A (1984) Coexistence of peptide HI (PHI) and VIP in nerves regulating blood flow and bronchial smooth muscle tone in various mammals including man. Peptides 5:593–606
Lundberg JM, Martling C-R, Höfkelt T (1988) Airways, oral cavity and salivary glands: classical transmitters and peptides in sensory and autonomic motor neurons. In: Bjbrklund A, Hökfelt T, Owman C (eds) Handbook of chemical neuroanatomy. Elsevier, Amsterdam, pp 391–444
Luts A, Sundler F (1989) Peptide-containing nerve fibers in the respiratory tract of the ferret. Cell Tissue Res 258: 259–267
Raufman J-P, Jensen RT, Sutcliff VE, Pisano JJ, Gardner JD (1982) Actions of Gila monster venom on dispersed acini from guinea pig pancreas. Am J Physiol 242: G470-G474
Stefanini M, Martino C de, Zamboni L (1967) Fixation of ejaculated spermatozoa for electron microscopy. Nature 216: 173
Sundler F, Ekblad E, Grunditz T, Hakanson R, Uddman R (1988) Vasoactive intestinal peptide in the peripheral nervous system. In: Said S, Mutt V (eds) vasoactive intestinal peptide and related peptides. Ann NY Acad Sci 527:143–167
Uddman R, Alumets J, Densert O, Hakanson R, Sundler F (1978) Occurrence and distribution of VIP nerves in the nasal mucosa and tracheobronchial wall. Acta Otolaryngol (Stockh) 86:443–448
Vandermeers A, Gourlet P, Vandermeers-Piret M-C et al. (1987) Chemical, immunological and biological properties of peptides like vasoactive-intestinal-peptide and peptide-histidineisoleucinamide extracted from the venom of two lizards (Heloderma horridum and Heloderma suspectum). Eur J Biochem 164:321–327
Zamboni L, Martino C de (1967) Buffered picric acid formaldehyde: a new, rapid fixative for electron microscopy. J Cell Biol 35:148A
Zhou ZC, Gardner JC, Jensen RT (1989) Interaction of peptides and related to VIP and secretin with guinea pig pancreatic acini. Am J Physiol 256: pG283–290
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Dedicated to the memory of Prof. Heinrich Spoendlin (Innsbruck)
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Hauser-Kronberger, C.E., Backer, G.W., Sundler, F. et al. Distribution and co-localization of immunoreactive helospectin with vasoactive intestinal polypeptide and peptide histidine methionine in human nasal mucosa, soft palate and larynx. Eur Arch Otorhinolaryngol 249, 201–205 (1992). https://doi.org/10.1007/BF00178469
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DOI: https://doi.org/10.1007/BF00178469