Summary
The effect of a low dose of diclofenac sodium, administered locally, on heterotopic bone formation in rats was investigated. Heterotopic bone was induced by implantation of demineralised bone matrix into the gluteal muscles. Diclofenac was released continuously for 2 weeks from micro-osmotic pumps for each demineralised bone implant in the rats. Each had a control implant in the opposite gluteal muscle to which saline was released in the same way. The yield of new woven bone was determined after 4 weeks by measuring the ash-weights of the implants. Those of the diclofenac samples were significantly decreased compared to the controls. These results suggest that a low dose of diclofenac given locally decreases heterotopic bone formation in rats.
Résumé
L'effet, sur la formation d'os hétérotopic d'une petite dose de diclofenac sodium administrée localement a été étudié chez le rat. Les ossifications hétéropiques ont été crées par l'implantation d'une matrice osseuse déminéralisée dans le muscle fessier des rats. Le diclofenac (0,2 microgramme par ml) a été délivré de façon continue pendant 2 semaines par des pompes micro-osmotiques (200 μl) au niveau du fragment d'os déminéralisé. Chaque animal a eu un fragment d'os déminéralisé de contrôle placé dans le muscle fessier contro-latéral et au niveau duquel une solution saline (0,9% NaCl) a été administrée de la même façon. La formation d'os nouveau a été déterminée après 4 semaines par la mesure du poids des fragments osseux réduits en cendres. Le poids des cendres des échantillons traités au diclofenac est significativement plus faible que celui des contrôles (p inférieur à 0,05). Nos résultats indiquent qu'une faible dose de diclofenac administrée localement, décroit expérimentalement la formation d'os hétérotopic chez le rat.
Article PDF
Similar content being viewed by others
References
Aspenberg P, Lohmander LS (1989) Fibroblast growth factor stimulates bone formation. Bone induction studied in rats. Acta Orthop Scand 60: 473–476
Delloye C, Hebrant A, Munting E, Piret L, Coutelier L (1985) The osteoinductive capacity of differently HCl-decalcified bone alloimplants. Acta Orthop Scand 56: 318–322
Elmstedt E, Lindholm TS, Nilsson OS, Törnkvist H (1985) Effect of ibuprofen on heterotopic ossification after hip replacement. Acta Orthop Scand 56: 25–27
Høgevold HE, Grøgaard B, Reiker»s O (1993) The effects of short-term and long-term treatment with indomethacin on primary and secondary healing in rats. Eur J Musculoskel Res 2: 3–8
Kjaersgaard-Andersen P, Nafei A, Teichert G, Kristensen O, Schmidt SA, Keller J, Lucht U (1993) Indomethacin for prevention of heterotopic ossification: a randomized controlled study in 41 hip arthroplasties. Acta Orthop Scand 64: 639–642
Menassé R, Hedwall PR, Kraetz J, Percin C, Reisterer L, Sallman A, Ziel R, Jaques R (1978) Pharmacological properties of diclofenac sodium and its metabolites. Scand J Rheumatol [Suppl] 22: 5–16
Nilsson OS, Bauer HCF, Brosjö O, Törnkvist H (1986) Influence of indomethacin on experimental bone formation in rats: Importance of length of treatment and of age. Clin Orthop 207: 239–245
Nilsson OS, Bauer HCF, Brosjö O, Törnkvist H (1987) A comparision of indomethacin and diclofenac in the inhibition of experimental heterotopic new bone formation. Int Orthop 11: 283–287
Rø J, Sudmann E, Marton PF (1976) Effect of indomethacin on fracture healing in rats. Acta Orthop Scand 47: 588–599
Schmidt SA, Kjaersgaard-Andersen P, Pedersen NW, Kristensen SS, Pedersen P, Nielsen J (1988) The use of indomethacin to prevent the formation of heterotopic bone after total hip replacement. J Bone Joint Surg [Am] 70: 834–838
Sudmann E, Bang G (1979) Indomethacin-induced inhibition of Haversian remodelling in rabbits. Acta Orthop Scand 50: 621–627
Törnkvist H, Nilsson OS, Bauer HCF, Lindholm TS (1983) Experimentally induced heterotopic ossification in rats influenced by anti-inflammatory drugs. Scand J Rheumatol 12: 177–180
Urist MR (1965) Bone: Formation by autoinduction. Science 150: 893–899
Wahlström O, Djerf K, Risto O, Hammerby S (1991) Diclofenac as prophylaxis against heterotopic bone formation after hip arthroplasty. Acta Orthop Scand 62: 419–421
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Risto, O., Wahlström, O. & Abdiu, A. The effect of low dose diclofenac sodium administered locally on heterotopic bone formation in rats. International Orthopaedics 19, 392–395 (1995). https://doi.org/10.1007/BF00178357
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00178357