, Volume 92, Issue 2, pp 236–240 | Cite as

Endurance training effects on striatal D2 dopamine receptor binding and striatal dopamine metabolites in presenescent older rats

  • P. G. MacRae
  • W. W. Spirduso
  • T. J. Walters
  • R. P. Farrar
  • R. E. Wilcox
Original Investigations


Endurance training is associated with higher binding of 3H-spiperone to striatal D2 dopamine receptors of rats sacrificed 48 h following the last exercise bout (Gilliam et al. 1984). In the present study we investigated the effects of endurance training in presenescent older rats on the relationship between steady-state levels of DA and its metabolites in striatum versus the affinity and density of striatal D2 DA receptors. Citrate synthase activity of the gastrocnemius-plantaris muscle was 29.06±2.27 μmole/g wet wt in 21-month-old trained rats versus 22.88±1.13 μmole/g wet wt in 21-month-old untrained animals.

DOPAC levels and DOPAC/DA ratios were greater in the old controls. Endurance training was associated with lower DOPAC levels in the 21-month-old animals. Thus, endurance training may postpone selectively changes in DA metabolism over a portion of the lifespan.

As expected, the number of D2 DA binding sites was reduced with age (6 months Bmax:429±21 fmoles/mg protein; 21 months:355±20) with no change in affinity. The Bmax of old runners was significantly higher (457 ± 38 fmoles/mg protein) than that of old controls. Thus, endurance training appears to exert a protective effect on D2 dopamine receptors during the lifespan. Taken together, the present results suggest that there may be a possible reciprocal relationship between changes in DA metabolites and DA binding as a function of exercise in presenescent older rats, and that endurance training may decelerate the effects of age both on nigrostriatal dopamine neurons and on striatal D2 dopamine receptors during a portion of the lifespan.

Key words

Basal ganglia D2 dopamine receptor Dopamine metabolites Endurance training Aging 


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  1. Barchas JD, Freedman DX (1963) Brain amines: response to physiological stress. Biochem Pharmacol 12:1232–1235Google Scholar
  2. Black HR (1979) Nonpharmacologic therapy for hypertension. Am J Med 66:837Google Scholar
  3. Blair SN, Goodyear NN, Gibbons LW (1984) Physical fitness and incidence of hypertension in healthy normotensive men and women. JAMA 252:487Google Scholar
  4. Brown BS, Van Huss W (1973) Exercise and rat brain catcholamine. J Appl Physiol 34(5):664–669Google Scholar
  5. Brown BS, Payne T, Kim C, Moore G, Krebs P, Martin W (1979) Chronic response of rat brain norepinephrine and serotonin levels to endurance training. J Appl Physiol: Respirat Environ Exercise Physiol 46(1):19–23Google Scholar
  6. Butler J, O'Brien M, O'Malley K, Kelly JG (1982) Relationship of β-adrenoceptor density to fitness in athletes. Nature 298:60–62Google Scholar
  7. deCastro JM, Duncan G (1985) Operantly conditioned running: effects on brain catecholamine concentrations and receptor densities in the rat. Pharmacol Biochem Behav 23:495–500Google Scholar
  8. Dustman RE, Ruhling RO, Russell EM, Shearer DE, Bonekat W, Shigeoka JW, Wood JS, Bradford DC (1984) Aerobic exercise training and improved neuropsychological function of older individuals. Neurobiol Aging 5:35–42Google Scholar
  9. Gilliam PE, Spirduso WW, Martin TP, Walters TJ, Wilcox RE, Farrar RP (1984) The effects of exercise training on 3H-spiperone binding in rat striatum. Pharmacol Biochem Behav 20:863–867Google Scholar
  10. Gordon R, Spector S, Sjoerdsma A, Udenfriend S (1966) Increased synthesis of norepinephrine and epinephrine in the intact rat during exercise and exposure to cold. J Pharmacol Exp Ther 153:440–447Google Scholar
  11. Hamblin MW, Leff SE, Creese I (1984) Interactions of agonists with D2 dopamine receptors: evidence for a single receptor population existing in multiple agonist affinity states in rat striatal membranes. Biochem Pharmacol 33:877–887Google Scholar
  12. Heyes MP, Garnett ES, Coates G (1985) Central dopaminergic activity influences rats ability to exercise. Life Sci 36:671–677Google Scholar
  13. Limbird LL (1985) Cell surface receptors: a short course on theory and methods. Boston, Nijhoff, pp 1–196Google Scholar
  14. Lowry OH, Rosebrough I, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–273PubMedGoogle Scholar
  15. Lukaszyk A, Buczko W, Wisniewski K (1983) The effect of strenuous exercise on the reactivity of the central dopaminergic system in the rat. Pol J Pharmacol Pharm 35:29–36Google Scholar
  16. McMaster SB, Carney JM (1985a) Exercise-induced changes in scheduled controlled behavior. Physiol Behav 35:337–341Google Scholar
  17. McMaster SB, Carney JM (1985b) Changes in drug sensitivity following acute and chronic exercise. Pharmacol Biochem Behav 23:191–194Google Scholar
  18. Paffenbarger RS Jr, Wing AL, Hyde RT, Jung DL (1983) Physical activity and incidence of hypertension in college allumni. Am J Epidemiol 117:245–257Google Scholar
  19. Paffenbarger RS Jr, Hyde RT, Wing AL, Hseih CC (1986) Physical activity, all-cause mortality, and longevity of college alumni. New Engl J Med 314:605–613Google Scholar
  20. Randall PK (1980) Functional aging of the nigrostriatal system. Peptides [Suppl 1] 1:177–184Google Scholar
  21. Rogers J, Bloom FE (1985) Neurotransmitter metabolism and function in the aging central nervous system. In: Finch CE, Schneider EL (eds) Handbook of the biology of aging (2nd edition). New York, Van Nostrand Reinhold, pp 645–691Google Scholar
  22. Samorajski T, Delaney C, Durham L, Ordy JM, Johnson JA, Dunlap WP (1985) Effect of exercise on longevity, body weight, locomotor performance, and passive-avoidance memory of C57/BL6J mice. Neurobiol. Aging 6:17–24Google Scholar
  23. Scatchard G (1949) The attractions of proteins for small molecules and ions. Ann NY Acad Sci 51:660–672Google Scholar
  24. Scheuer J, Tipton CM (1977) Cardiovascular adaptations to physical training. Annu Rev Physiol 39:221Google Scholar
  25. Semenova TP, Ivanov VA, Tretyak TM (1981) Brain levels of noradrenaline, dopamine and serotonin in rats with different levels of motor activity. Neurosci Behav Physiol 2:153–155Google Scholar
  26. Severson JA, Finch CE (1980) Reduced dopaminergic binding during aging in the rodent striatum. Brain Res 192:147–162Google Scholar
  27. Severson JA, Randall PK (1985) D2 Dopamine receptors in aging mouse striatum: determination of high- and low-affinity agonist binding sites. J Pharmacol Exp Ther 233:361–368Google Scholar
  28. Srere PA (1969) Citrate synthase. In: Lowenstein JM (eds) Methods in enzymology 13. New York, Academic, pp 3–5Google Scholar
  29. Wilcox RE (1984) Changes in biogenic amines and their metabolites with aging and alcoholism. In: Hartford JT, Samorajski T (eds) Alcoholism in the elderly. New York, Raven, pp 85–115Google Scholar
  30. Wilcox RE, Hightower WH, Smith RV (1979) Post-hoc data analysis in biomedical research. Am Lab 11:32–45Google Scholar
  31. Wilcox RG, Bennett T, Brown AM (1982) Is exercise good for high blood pressure? Br Med J 285:767Google Scholar
  32. Winer BJ (1971) Statistical principles in experimental design. New York, McGraw-HillGoogle Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • P. G. MacRae
    • 1
  • W. W. Spirduso
    • 1
    • 2
  • T. J. Walters
    • 1
  • R. P. Farrar
    • 1
    • 2
  • R. E. Wilcox
    • 2
  1. 1.Department of Health and Physical EducationUniversity of TexasAustinUSA
  2. 2.Institute for Neuroscience, Department of Pharmacology and Toxicology, College of PharmacyUniversity of TexasAustinUSA

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