Skip to main content
SpringerLink
Log in

DNA ploidy and S-phase in recurrent astrocytomas: a retrospective study by flow cytometry of deparaffinized specimens

  • Laboratory Investigation
  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Summary

Twenty-two patients with recurrent astrocytic tumors were treated surgically two or even three times. At the time of the first surgery 6 tumors were fibrillary astrocytomas (grade II), 9 anaplastic astrocytomas (grade III) and 7 glioblastomas (grade IV).

Histopathological specimens from second surgery demonstrated in 12 cases signs of higher grades of malignancy. Flow cytometry (FCM) did not reveal any significant changes of S-phase fraction (p = 0.55). This study supports the theory that, given enough time, the histopathology of brain tumors change significantly from more benign forms to more malignant ones. The flow cytometry (FCM) could trace a weak tendency to higher S-phase fractions at the time of the second surgery. No apparent change of ploidy pattern was observed. In spite of the unequivocal histopathological changes of the recurrent astrocytomas the flow cytometry failed to indicate similar changes in terms of ploidy and S-phase fraction parameters.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ahyai A: Flow cytometric analysis of cellular DNA content in human astrocytomas and oligodendrogliomas. Neurosurg Rev 11: 177–187, 1988

    Google Scholar 

  2. Bowles AP, Pantazis CP, Allen MM, Martinez J, Allsbrook WC: Ganglioglioma, a malignant tumor? Correlation with flow deoxyribonucleic acid cytometric analysis. Neurosurgery 23: 376–381, 1988

    Google Scholar 

  3. Crone KR, Challa VR, Knute TE, Moody DM, Kelly DL: Relationship between flow cytometric features and clinical behavior of meningeomas. Neurosurgery 23: 720–724, 1988

    Google Scholar 

  4. Kros JM, van Eden CG, Vissers CJ, Mudler AH, van der Kwast ThH: Prognostic relevance of DNA flow cytometry in oligodendroglioma. Cancer 69: 1791–1798, 1992

    Google Scholar 

  5. Giangaspero F, Chieco P, Lisignoli G, Burger PC: Comparison of cytological composition with microfluorometric DNA analysis of the glioblastoma multiforme and anaplastic astrocytoma. Cancer 60: 59–65, 1987

    Google Scholar 

  6. Nagashima T, Hoshino T, Cho KG, Senegor M, Waldman F, Nomura K: Comparison of bromodeoxyuridine labeling indices obtained from tissue sections and flow cytometry of brain tumors. J Neurosurg 68: 388–392, 1988

    Google Scholar 

  7. De Reuck J, Sieben G, Coster W, Roels H, Van der Eeken H: Cytophotometric DNA determination in human oligodendroglial tumors. Histopathology 4: 225–232, 1980

    Google Scholar 

  8. Salmon I, Kiss R, Dewitte O, Gras T, Pasteels J-L, Brotchi J, Flament-Durant J: Histopathologic grading and DNA ploidy in relation to survival among 206 adult astrocytic tumor patients. Cancer 70(2): 538–546, 1992

    Google Scholar 

  9. Danova M, Riccardi A, Mazzini G, Ucci G, Montecucco MC, Gaetani P, Silvani V, Knerich R, Butti G, Ascari E, Paoletti P: Proliferative characteristics and ploidy of human brain tumors by DNA flow cytometry. Bas Appl Histochem 30: 175–180, 1986

    Google Scholar 

  10. Hoshino T, Nomura K, Wilson CB, Knebel KD, Gray JW: The distribution of nuclear DNA from human brain-tumor cells. J Neurosurg 49: 13–21, 1978

    Google Scholar 

  11. Hoshino T, Nagashima T, Murovic JA, Wilson CB, Edwards MSB, Gutin PH, Davis RL, De Armond SJ: In situ kinetic studies with bromodeoxyuridine labeling. J Neurosurg 64: 453–459, 1986

    Google Scholar 

  12. Hoshino T, Rodriguez LA, Cho KG, Lee KS, Wilson CB, Edwards SB, Levin VA, Davis RL: Prognostic implications of the proliferative potential of low-grade astrocytomas. J Neurosurg 69: 839–842, 1988

    Google Scholar 

  13. Hoshino T, Prados M, Wilson CB, Cho KG, Lee K-S, Davis RL: Prognostic implications of the bromodeoxyuridine labeling index of human gliomas. J Neurosurg 71: 335–341, 1989

    Google Scholar 

  14. Nishizaki T, Orita T, Furutani Y, Ikeyama Y, Aoki H, Sasaki K: Flow cytometric DNA analysis and immunohistochemical measurement of Ki-67 and BUdR labeling indices in human brain tumors. J Neurosurg 70: 379–384, 1989

    Google Scholar 

  15. Nishizaki T, Orita T, Kajiwara K, Ikeda N, Ohshita N, Nakayama H, Kamiryo T, Ito H: Correlation of in vitro bromodeoxyuridine labeling index and DNA aneuploidy with survival or recurrence in brain-tumor patients. J Neurosurg 73: 396–400, 1990

    Google Scholar 

  16. Nishizaki T, Ohshita N, Nagatsugu Y, Orita T, Ito H, Sasaki K: Clinical evaluation of DNA index in human brain tumors. J Neuro-Oncol 17(1): 9–13, 1993

    Google Scholar 

  17. Raghavan R, Steart PV, Weller RO: Cell proliferation patterns in the diagnosis of astrocytomas, anaplastic astrocytomas and glioblastoma multiforme: a Ki-67 study. Neuropathol & App1 Neurobiol 16(2): 123–133, 1990

    Google Scholar 

  18. Salcman M, Kaplan RS, Ducker TB, Abdo H, Montgomery E: Effect of age and reoperation on survival in the combined modality treatment of malignant astrocytoma. Neurosurgery 10(4): 454–463, 1982

    Google Scholar 

  19. Vavruch L, Eneström S, Carstensen J, Nordenskjöld B, Wingren S: DNA index and S-phase in primary brain tumors. J Neurosurg 80: 85–89, 1994

    Google Scholar 

  20. Kleihues P, Burger PC, Scheithauer BW: Histological Typing of Tumors of the Central Nervous System. 2nd edition. Berlin, Springer-Verlag, 1993

    Google Scholar 

  21. Vindelov LL, Christensen IJ, Nissen NI: A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry 3: 323–327, 1983

    Google Scholar 

  22. Wingren S, Hatschek T, Staal O, Boeryd B, Nordenskjöld B: Comparison of static and flow cytofluometry for estimation of DNA index and S-phase fraction in fresh and paraffin-embedded breast carcinoma tissue. Acta Oncol 27: 793–797, 1989

    Google Scholar 

  23. Goerttler K: Durchflusszytometrische Untersuchungen zum Proliferationsverhalten menschlicher Tumoren. Acta Histochem Suppl 39: 40–60, 1990

    Google Scholar 

  24. Armitage P, Berry G: Statistical Methods in Medical Research. 2nd edition. Oxford: Blackwell, 1987

    Google Scholar 

  25. Coons SW, Johnson PC: Regional heterogeneity in the DNA content of human gliomas. Cancer 72(10): 3052–3060, 1993

    Google Scholar 

  26. Coons SW, Davis JR, Way DL: Correlation of DNA content and histology in prognosis of astrocytomas. Am J Clin Pathol 90: 289–293, 1988

    Google Scholar 

  27. Friedlander ML, Hedley DW, Taylor JW: Clinical and biological significance of aneuploidy in human tumors. J Clin Pathol 37: 961–974, 1984

    Google Scholar 

  28. Appley AJ, Fitzgibbons PL, Chandrasoma PT, Hinton DR, Apuzzo MLJ: Multiparameter flow cytometric analysis of neoplasms of the central nervous system: correlation of nuclear antigen p105 and DNA content with clinical behavior. Neurosurgery 27: 83–96, 1990

    Google Scholar 

  29. van Meyel DJ, Ramsay DA, Casson AG: p53 mutation, expression, and DNA ploidy in evolving gliomas: evidence for two pathways of progression. J Nat Cancer Inst 86(13): 1011–1017, 1994

    Google Scholar 

  30. Salmon I, Dewitte P, Pasteels JL, Flament-Durand J, Brotchi J, Vereerstraeten P, Kiss R: Prognostic scoring in adult astrocytic tumors using patient age, histopathological grade, and DNA histogram type. J Neurosurg 80: 877–883, 1994

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Spinal Surgery, University Hospital, S-581 85, Linköping, Sweden

    Ludek Vavruch

  2. Department of Oncology, University Hospital, S-581 85, Linköping, Sweden

    Bo Nordenskjöld

  3. Department of Pathology, University Hospital, S-581 85, Linköping, Sweden

    John Carstensen

  4. Department of Health and Society, Linköping University, S-581 83, Linköping, Sweden

    Sverker Eneström

Authors
  1. Ludek Vavruch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bo Nordenskjöld
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John Carstensen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sverker Eneström
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vavruch, L., Nordenskjöld, B., Carstensen, J. et al. DNA ploidy and S-phase in recurrent astrocytomas: a retrospective study by flow cytometry of deparaffinized specimens. J Neuro-Oncol 30, 37–45 (1996). https://doi.org/10.1007/BF00177441

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00177441

Key words

Search

Navigation