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Cell-mediated cytotoxicity in glioma-bearing patients: Differential responses of peripheral blood mononuclear cells to stimulation with interleukin-2 and microbial antigen

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Abstract

Peripheral blood mononuclear cells (PBMC) of malignant glioma-bearing patients were stimulated in vitro with Interleukin-2 (ILr2) or a glucomannan-protein antigen of Candida albicans (GMP) then assayed for proliferation, production of IFN-gamma, and generation of cytotoxic effectors against either K562 tumor cell line or freshly-cultured allogenic glioma cells. PBMC of healthy, age and sex-matched subjects were the controls. PBMC of glioma-bearing patients did not differ, as a whole, from PBMC of healthy donors in IL-2 or GMP-induced proliferation. However, they showed a lesser ability to produce IFN as well as a substantial inability to generate cytotoxic effectors following GMP stimulation. PBMC of glioma patients were fully responsive to ILr2 in cytotoxicity generation, as were the PBMC from normal subjects. The results suggest that glioma patients may have a defective antigen-mediated activation of natural cytotoxic effectors. This hyporesponsiveness is not accompanied by depressed lymphoproliferation and does not apparently involve a reduced response to IL-2.

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Authors and Affiliations

  1. Istituto CNR Tipizzazione Tissutale e Problemi della Dialisi, LAquila, Italy

    C. Ausielloh

  2. Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanity, Roma, Italy

    C. Ausielloh & A. Cassone

  3. Dipartimento di Scienze Neurologiche, Sezione di Neurochirurgia, University di Roma'La Sapienza, Italy

    A. Maleci

  4. Istituto di Virologia, University di Roma ‘La Sapienza’, Roma, Italy

    G. C. Spagnolil & G. Antonelli

Authors
  1. C. Ausielloh
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  2. A. Maleci
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  3. G. C. Spagnolil
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  4. G. Antonelli
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  5. A. Cassone
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Ausielloh, C., Maleci, A., Spagnolil, G.C. et al. Cell-mediated cytotoxicity in glioma-bearing patients: Differential responses of peripheral blood mononuclear cells to stimulation with interleukin-2 and microbial antigen. J Neuro-Oncol 6, 329–338 (1988). https://doi.org/10.1007/BF00177428

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  • DOI: https://doi.org/10.1007/BF00177428

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