Skip to main content
SpringerLink
Log in

Radioimaging of human glioma xenografts with 123I labeled monoclonal antibody G-22 against glioma-associated antigen

  • Laboratory Investigation
  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Summary

Monoclonal antibody (MCA) G-22 is directed against a human glioma-associated surface antigen. Its availability for the radioimmunodetection of human glioma was analyzed by utilizing the xenografts in athymic mice. Nude mice with subcutaneous grafts of U251-MG or U251-SP glioma received intravenous administration of 123I or 131I labeled F(ab′)2 fragment or whole immunoglobulin. Results of radioimaging revealed that 123I-labeled antibody was better than the 131I-labeled. It was also noted that administration of 123I-labeled F(ab′)2 fragment of G-22 MCA enabled the imaging of human glioma xenografts weighing 80–650 mg after 48 hours. When biodistribution of 123I MCA was compared between G-22 and control antibodies, the percentages of dose/g in tumors were 5.228–1.799 at 30 hours and 4.112–1.132 at 48 hours with G-22 and they were 4.164–1.248 and 0.314–0.142 with control. The tumor/blood ratio until 72 hours after injection was constantly above 1 with G-22 and less than 1 with control antibody. These results indicate the potential usefulness of G-22 MCA for the radioimmunodetection of human gliomas.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pressman D, Keighley G: The zone of activity of antibodies as determined by the use of radioactive tracers. The zone of activity of nephritoxic antikidney serum. J Immunol 59: 141–146, 1948

    Google Scholar 

  2. Pressman D, Pressman R: Computer programs for paired and tried radioiodine label techniques in radioimmunochemistry. Int J Appl Radiat Isot 18: 617–622, 1965

    Google Scholar 

  3. Goldenberg DM, Preston DF, Primus FJ, Hansen HJ: Photoscan localization of GW-39 tumors in hamsters using radiolabeled anticarcinoembryonic antigen immunoglobulin G. Cancer Res 34: 1–9, 1974

    Google Scholar 

  4. Mach JP, Carrel S, Merenda C, Sordat B, Cerottini JC: In vivo localization of radiolabeled antibodies to carcinoembryonic antigen in human colon carcinoma grafted into nude mice. Nature 248: 704–706, 1974

    Google Scholar 

  5. Goldenberg DM, Deland F, Kim E, Berrnett S, Primus FJ, van Nagell JR, Estes N, DeSimone P, Rayburn P: Use of radiolabeled antibodies to carcinoembryonic antigen for the detection and localization of diverse cancer by external photoscanning. N Eng J Med 298: 1384–1388, 1978

    Google Scholar 

  6. Mach JP, Form M, Ritchard J, Buchegger F, Carrel S, Widgren S, Donath A, Alberto P: Use and limitation of radiolabeled anti-CEA antibodies and their fragments for photoscanning detection of human colorectal carcinoma. Oncodev Biol Med 1: 49–69, 1980

    Google Scholar 

  7. Goldenberg DM, Kim EE, DeLand FH, Spremueli E, Nelson MD, Gockerman JP, Primus FJ, Corgan RL, Alpert E: Clinical studies on the radioimmunodetection of tumors containing alpha-fetoprotein. Cancer 45: 2500–2505, 1980

    Google Scholar 

  8. Day ED, Lassiter S, Woodhall B, Mahaley JL, Mahaley MS: The localization of radioantibodies in human brain tumors. 1. Preliminary exploration. Cancer Res 25: 773–778, 1965

    Google Scholar 

  9. Mach J-P, Buchegger F, Form M, Ritschand J, Berche C, Lumbroso J-D, Schreyer M, Girardet C, Accolla RS, Carrel S: Use of radiolabeled monoclonal anti-CEA antibodies for the detection of human carcinomas by external photoscanning and Fomoscintigrophy. Immunol Today 2: 239–249, 1981

    Google Scholar 

  10. Moldofsky PJ, Powe J, Mulhern CB Jr, Hammond N, Sears HF, Gatenby RA, Steplewski Z, Koprowski H: Metastatic colon carcinoma detected with radiolabeled F(ab′)2 monoclonal antibody fragments. Radiology 149: 549–555, 1983

    Google Scholar 

  11. Larson SM, Brown JP, Wright PW, Carrasquillo JA, Hellstrom I, Hellstrom KE: Imaging of melanoma with I-131-labeled monoclonal antibodies. J Nucl Med 24: 123–129, 1983

    Google Scholar 

  12. Farrands PA, Perkins A, Sully L, Hopkins JS, Pimm MV, Baldwin RW, Hardcastle JD: Localization of human osteosarcoma by antitumor monoclonal antibody. Journal of Bone and Joint Surgery 65-B: 638–640, 1983

    Google Scholar 

  13. Epenetos AA, Mather S, Granowska M, Nimmon CC, Hawkins LR, Britton KE, Shepherd J, Taylor-Paradimitrion J, Durbin H, Malpas JS, Bodmer WF: Targeting of iodine-123-labeled tumor-associated monoclonal antibodies to ovarion, breast, and gastrointestinal tumors. Lancet 11: 999–1006, 1980

    Google Scholar 

  14. Davis AG, Richardson PB, Bourne SP, Kemshead JT, Coakham HB: Immunolocalization of human brain tumors. In: Bleehan N (ed) Tumor of the Brain. Springer-Verlag, New York, pp 83–99, 1986

    Google Scholar 

  15. Yoshida J, Kageyama N, Ueda R, Takahashi T: Human glioma associated and related antigens identified by monoclonal antibodies. Neurosurg Rev 7: 23–28, 1984

    Google Scholar 

  16. Yoshida J, Wakabayashi T, Kito A, Kageyama N, Murata Y, Seo H, Kojima N, Yagi K: Clinical application of monoclonal antibodies against glioma-associated antigen. Prog Exp Tumor Res 30: 44–56, 1987

    Google Scholar 

  17. Wakabayashi T, Yoshida J, Seo H, Kazo K, Murata Y, Matui N, Kageyama N: Characterization of neuroectodermal antigen by a monoclonal antibody and its application in CSF diagnosis of human glioma. J Neurosurg 68: 449–455, 1988

    Google Scholar 

  18. Kito A, Yoshida J, Kageyama N, Kojima N, Yagi K: Liposomes coupled with monoclonal antibody against glioma-associated antigen for targeting chemotherapy of glioma. J Neurosurg 71: 382–387, 1989

    Google Scholar 

  19. Yoshida J, Yamamoto R, Wakabayashi T, Nagata M, Seo H: Radioimmunoassay of glioma-associated antigen in cerebrospinal fluid and its usefulness for the diagnosis and monitoring of human glioma. J Neuro-Oncology, in press

  20. Westermark B, Pontèn J, Hugosson R: Determinants for the establishment of permanent tissue culture lines from human gliomas. Acta path microbiol scan Section A 81: 791–805, 1973

    Google Scholar 

  21. Mihara Y: Establishment and characterization of 5 cell lines derived from human brain tumors. Kumamoto Med 139: 25–41, 1986

    Google Scholar 

  22. Pettit WA, Berrnet SJ, Deland F, Goldenberg DM: Iodination and acceptance testing of antibodies. In: Burchiel SW, Rhodes BA, Friedman BE (eds) Tumor Imaging, The radioimmunochemical detection of cancer. Masson Publishing USA, Inc 1989, pp 99–109

  23. Richardson RB, Davies AG, Bourne SP, Staddon GE, Jones DH, Kemshead IT, Coakham HB: Radioimmunolocalization of human brain tumors: Biodistribution of radio labeled monoclonal antibody UJ 13A. Eur J Nucl Med 12: 313–320, 1986

    Google Scholar 

  24. Wikstrand LJ, Mclendon RE, Bullard DE, Fredman P, Svennerholm L, Bigner DD: Production and characterization of two glioma xenograft-localizing monoclonal antibodies. Cancer Res 46: 5933–5940, 1986

    Google Scholar 

  25. Bourdon MA, Coleman RE, Blasberg RG, Groothuis DR, Bigner DD: Monoclonal antibody localization in subcutaneous and intracranial human glioma xenografts: paired-label and imaging analysis. Anticancer Res 4: 133–140, 1984

    Google Scholar 

  26. Lee YS, Bullard DE, Humphrey PA, Colapinto EV, Friedman HS, Zalutsky MR, Coleman PE, Bigner DD: Treatment of intracranial human glioma xenografts with 131-I-labeled Anti-Tenascin monoclonal antibody 81C6. Cancer Res 48: 2904–2910, 1988

    Google Scholar 

  27. Lee YS, Bullard DE, Wikstrand CJ, Zalutslcy MR, Muhlbaier LH, Bigner DD: Comparison of monoclonal antibody delivery to intracranial glioma xenografts by intravenous and intracarotid administration. Cancer Res 47: 1941–1946, 1987

    Google Scholar 

  28. Woo DV, Brady LW, Herlyn DM, Takahashi H, Miyamoto C, Karlsson U, Dadpravar S, Koprowski H: Radioimmunodetection of human glioma by a monoclonal antibody specific to epidermal growth factor receptor. Proc of ASCO 7: 83, 1988

    Google Scholar 

  29. Stavrou D, Glassner K, Bilzer T, Senekowitsch R, Keiditsch E, Mehraein P: Radioimaging of experimental glioma grafts using F(ab′)2 fragments of monoclonal antibodies. Anti Cancer Res 6: 897–903, 1986

    Google Scholar 

  30. Coakham HB: The application of monoclonal antibodies in the diagnosis and therapy of brain tumors. Proceedings of the Fourth Meeting of the Japan Society of Brain Tumor Pathology. 155–164,1986

  31. Lashford LS, Davies AG, Richardson RB, Bourne SP, Bullimore JA, Eckert H, Kemshead JT, Coakham HB: A pilot study of 131I monoclonal antibodies in the therapy of leptomeningeal tumors. Cancer 61: 857–868, 1988

    Google Scholar 

  32. Zelutsky MR, Moseley RP, Coakham HB, Coleman E, Bigner DD: Pharmacokinetics and tumor localization of 131I-labeled anti-tenascin monoclonal antibody 81C6 in patients with gliomas and other intracranial malignancies. Cancer Research 49: 2807–2813, 1989

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Neurosurgery, Nagoya University School of Medicine, Nagoya, Japan

    Jun Yoshida, Masaaki Mizuno, Itaru Inoue, Toshihiko Wakabayasi & Kenichiro Sugita

  2. Department of Endocrinology and Metabolism, Research Institute of Environmental Medicine, Nagoya, Japan

    Hisao Seo

  3. LTD, Daiichi Radioisotope Laboratories, Chiba, Japan

    Kazumi Chiba

Authors
  1. Jun Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masaaki Mizuno
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Itaru Inoue
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Toshihiko Wakabayasi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kenichiro Sugita
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hisao Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kazumi Chiba
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yoshida, J., Mizuno, M., Inoue, I. et al. Radioimaging of human glioma xenografts with 123I labeled monoclonal antibody G-22 against glioma-associated antigen. J Neuro-Oncol 8, 221–229 (1990). https://doi.org/10.1007/BF00177355

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00177355

Key words

Search

Navigation