Abstract
Retinal abnormalities have been described in both animals and humans exposed to cocaine during development. The present study was designed to examine the morphological repercussions of neonatal exposure to cocaine on the developing retina of the rat. Male Wistar rats were given 15 mg/kg body weight per day of cocaine hydrochloride subcutaneously on postnatal days (PND) 0–6, 13 and 29 and sacrificed at PND 7, 14 or 30; controls were given saline. The retinas were processed for electron microscopy. Retina quadrants were embedded flat and vertical semithin and ultrathin sections obtained. PND 7 sections showed discrete intraretinal hemorrhages, PND 14 sections showed massive intraretinal hemorrhages and images of ischemic necrosis in the nerve fiber layer and PND 30 sections showed cavity lesions in the hemorrhagic areas, gliosis and pigmented macrophagerich epiretinal membranes; photoreceptor rosettes were also found. These results are the first morphological demonstration of retinal hemorrhages and associated epiretinal membranes following neonatal exposure to cocaine in the rat. These changes are probably related to the ischemia/hypoxia induced by cocaine.
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References
Brendt RL (1990) Relationship between uterine vascular clamping, vascular disruption syndrome and cocaine teratogenicity. Teratology 41:757–760
Burke JM, Smith JM (1981) Retinal proliferation in response to vitreous hemoglobin or iron. Invest Ophthalmol Vis Sci 20:582–592
Clarkson JG, Green WR, Massof D (1977) A histopathologic review of 168 cases of preretinal membrane. Am J Ophthalmol 84:1–17
Crosby WH (1993) The vasoconstrictor action of cocaine. J Pharmacol Exp Ther 65:150–155
Dixon SD, Coem RW, Crutchfield gnS (1987) Visual dysfunction in cocaine-exposed infants. Pediatr Res 21:395A
obbing J, Sands J (1979) Comparative aspects of the brain growth spurt. Early Hum Dev 3:79–83
Dominguez RD, Vila-Coro AA, Slopis JM, Bohan TP (1991) Brain and ocular abnormalities in infants with in utero exposure to cocaine and other street drugs. Am J Dis Child 145:688–695
Ferriero DM, Kobori JA, Good WV, Golabi M (1989) Retinal defects in cocaine-exposed infants. Ann Neurol 26:458
Gingras JL, Weese-Mayer DE, Hume RF, Donnell KJ (1992) Cocaine and development: mechanisms of fetal toxicity and neonatal consequences of prenatal cocaine exposure. Early Hum Dev 31:1–24
Good WV, Ferriero DM, Golabi M, Kobori JA (1992) Abnormalities of the visual system in infants exposed to cocaine. Ophthalmology 99:341–346
Hughes WF (1991) Quantitation of ischemic damage in the rat retina. Exp Eye Res 53:573–582
Isenberg SJ, Spierer A, Inkelis S (1987) Ocular signs of cocaine intoxication in neonates. Am J Ophthalmol 103:211–214
Kiritsy-Roy JA, Halter JB, Gordon SM, Smith MJ, Terry LC (1990) Role of central nervous system in hemodynamic and sympathoadrenal responses to cocaine in rats. J Pharmacol Exp Ther 255:154–160
Palay SL, Chan-Palay V (1974) Cerebellar cortex - cytology and organization. Springer, Berlin Heidelberg New York
Seidler FJ, Slotkin TA (1993) Prenatal cocaine and cell development in rat brain regions: effects on ornithine decarboxylase and macromolecules. Brain Res Bull 30:91–99
Silva-Araújo A, Salgado-Borges J, Tavares MA (1991) Morphological changes in the optic nerve after chronic exposure of neonatal rats to cocaine and amphetamine. Ophthalmic Res 23:295–302
Silva-Araújo A, Salgado-Borges J, Cardoso V, Castro-Correia J, Tavares MA (1993) Changes in retinal ganglion cell layer and optic nerve of rats exposed neonatally to cocaine. Exp Eye Res 56:199–206
Tavares MA, Silva MC (1993) Body weight gain and hippocampal volumes of rats exposed prenatally to psychostimulants. Brain Res 619:137–145
Teske MP, Trese MT (1987) Retinopathy of prematurity-like fundus and persistent primary vitreous associated with maternal cocaine use. Am J Ophthalmol 103:719–720
Volpe JJ (1992) Effect of cocaine use on the fetus. N Engl J Med 327:399–407
Wallace RT, Brown GC, Benson W, Sivalingham A (1992) Sudden retinal manifestations of intranasal cocaine and methamphetamine abuse. Am J Ophthalmol 114:158–160
Webster WS, Brown-Woodman PDC (1990) Cocaine as a cause of congenital malformations of vascular origin: experimental evidence in the rat. Teratology 41:689–697
Webster WS, Woodman PDCB, Lipson AH, Ritchie HE (1991) Fetal brain damage in the rat following prenatal exposure to cocaine. Neurotoxicol Teratol 13:621–626
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This work was supported by grants from the JNICT Project (PMCT/C/SAU/10/90) and the STRIDE (FEDER) Project (STRAD/C/SAU/304/92)
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Silva-Araújo, A., Abreu-Dias, P., Salgado-Borges, J. et al. Retinal changes induced by neonatal cocaine exposure in the rat. Graefe's Arch Clin Exp Ophthalmol 232, 162–166 (1994). https://doi.org/10.1007/BF00176786
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DOI: https://doi.org/10.1007/BF00176786