Abstract
The binding of Cu and Cd ions with ovalbumin has been measured indirectly by observing the displacement of H+ from acidic groups on the ovalbumin. The pH-measuring procedure agreed with values obtained by an equilibrium dialysis method. Both the metals were bound by carboxyl groups with log K values being 2.39 and 2.22, respectively. The extent of binding was found to be pH dependent with the involvement of the imidazole site yielding logK values of 3.23 and 2.80, respectively. Equilibrium dialysis results supported involvement of both carboxyl and imidazole groups of the protein in metal ion binding.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arora, J. P. S., Jadon, D. S., and Singh, R. P.: 1980, J. Ind. Chem. Soc. 56, 1323.
Arora, J. P. S. and Singh, R. P.: 1981, Studia Biophysica 38, 181.
Arora, J. P. S., Singh, R. P., Kumar, S., and Singhal, V. K.: 1979, J. Prakt. Chem. 321, 164.
Gurd, F. R. N. and Goodman, D. S.: 1954, J. Am. Chem. Soc. 74, 670.
Gurd, F. R. N. and Murray, G. R., Jr.: 1954, J. Am. Chem. Soc. 76, 187.
Kotsonis, F. N., and Klassen, C. D.: 1981, in J. O. Nriagu (ed.), Metallothionein and Its Interaction with Cadmium in Cadmium Environment, Part II, John Wiley & Sons, Inc., New York.
Malik, W. U. and Arora, J. P. S.: 1969, Ind. J. Biochem. Biophys. 6, 142.
Malik, W. U. and Jindal, M. R.: 1968, J. Electroanal. Chem. 19, 436.
Malik, W. U. and Salahuddin: 1963, J. Electroanal. Chem. 5, 147.
Sabbioni, E., Marafante, E., Amatini, L., Ubertalli, L., and Pietra, R.: 1978, Science of the Total Environment 10, 135.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Verma, S.R., Arora, J.P.S., Shankar, J.S. et al. Interaction of cupric and cadmium ions with a protein-ovalbumin (OA). Water Air Soil Pollut 43, 53–59 (1989). https://doi.org/10.1007/BF00175582
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00175582