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Behavioral Ecology and Sociobiology

, Volume 34, Issue 1, pp 25–30 | Cite as

Task specialization in a wild bee, Apis florea (Hymenoptera: Apidae), revealed by RFLP banding

  • Benjamin P. Oldroyd
  • H. Allen Sylvester
  • Siriwat Wongsiri
  • Thomas E. Rinderer
Article

Abstract

Workers in a wild in situ colony of the dwarf honey bee, Apis florea, were observed undertaking the following behavior: liquid foraging, pollen foraging, guarding, stinging, fanning and wagging abdomen. Bees of each behavioral class were separately collected and frozen. Collections were made over a period of 10 days. Random samples of brood and workers were also collected. DNA was extracted from each bee and “fingerprinted” using a probe of unknown sequence obtained from an A. mellifera genomic library. Patterns of fingerprints (Fig. 1) were dissimilar among behavioral classes (Tables 1 and 2), strongly suggesting a genetic component to division of labor in this species. This result supports similar findings in A. mellifera in a species that is not troubled by many of the experimental difficulties inherent in A. mellifera.

Key words

Honey bee Subfamily RFLP Task specialization Dwarf honey bee Thailand Multiple mating 

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References

  1. Blanchetot AJ (1991) Genetic relatedness in honeybees as established by DNA fingerprinting. J Hered 82:391–396Google Scholar
  2. Calderone NW, Page JR Jr (1988) Genotypic variability in age polyethism and task specialization in the honey bee, Apis mellifera. Behav Ecol Sociobiol 22:17–25Google Scholar
  3. Calderone NW, Page RE Jr (1991) Evolutionary genetics of division of labor in colonies of the honey bee (Apis mellifera). Am Nat 138:69–92Google Scholar
  4. Calderone NW, Robinson GE, Page RE Jr (1989) Genetic structure and division of labor in honeybee colonies. Experientia 45:765–767Google Scholar
  5. Carlin NF, Frumhoff PC (1990) Nepotism in the honey bee. Nature 346:706–708Google Scholar
  6. Crozier RH, Page RE Jr (1985) On being the right size: male contributions and multiple mating in the social Hymenoptera. Behav Ecol Sociobiol 18:105–115Google Scholar
  7. Frumhoff PC (1991) The effects of the cordovan marker on apparent kin discrimination among nestmate honeybees. Anim Behav 42:845–856Google Scholar
  8. Frumhoff PC, Baker J (1988) A genetic component to division of labour within honey bee colonies. Nature 333:358–361Google Scholar
  9. Hamilton WD (1964) The genetical evolution of social behavior. J Theor Biol 7:11–16, 17–52Google Scholar
  10. Hogendoorn K, Velthuis HHW (1988) Influence of multiple mating on kin recognition by worker honey bees. Naturwissenschaften 75:412–413Google Scholar
  11. Koeniger N, Koeniger G, Wongsiri S (1989) Mating and sperm transfer in Apis forea. Apidologie 20:413–418Google Scholar
  12. Moritz RFA (1985) The effects of multiple mating on the worker-queen conflict in Apis mellifera L. Behav Ecol Sociobiol 16:375–377Google Scholar
  13. Moritz RFA, Hillesheim E (1989) Genetic intragroup variance and hoarding behavior in honey bees. Apidologie 20:383–390Google Scholar
  14. Moritz RFA, Meusel MS, Haberl M (1991) Oligonucleotide DNA ingerprinting discriminates super- and half-sisters in honeybee colonies (Apis mellifera L.). Naturwissenschaften 78:422–424Google Scholar
  15. Oldroyd BP, Rinderer TE, Buco SM (1992a) Intra-colonial foraging specialism by honey bees (Apis mellifera) (Hymenoptera: Apidae). Behav Ecol Sociobiol 30:291–295Google Scholar
  16. Oldroyd BP, Rinderer TE, Harbo JR, Buco SM (1992b) Effects of intracolonial genetic diversity on honey bee (Hymenoptera: Apidae) colony performance. Ann Entomol Soc Am 85:335–343Google Scholar
  17. Oldroyd BP, Rinderer TE, Buco SM, Beaman LD (1993) Genetic variance in honey bees for preferred foraging distance. Anim Behav 45:323–332Google Scholar
  18. Oster GF, Wilson EO (1978) Caste and ecology in the social insects. Princeton University Press, PrincetonGoogle Scholar
  19. Page RE Jr (1980) The evolution of multiple mating behavior in honey bee queens (Apis mellifera L.). Genetics 96:263–267Google Scholar
  20. Page RE Jr, Robinson GE (1991) The genetics of division of labor in the honey bee. Adv Insect Physiol 23:117–169Google Scholar
  21. Page RE Jr, Breed MD, Getz WM (1990) Nepotism in the honey bee. Nature 346:706–707Google Scholar
  22. Ratnieks FRW (1990) The evolution of polyandry by queens in social hymenoptera: the significance of the timing of removal of diploid males. Behav Ecol Sociobiol 28:343–348Google Scholar
  23. Robinson GE (1992) Regulated division of labor in insect societies. Annu Rev Entomol 37:635–637Google Scholar
  24. Robinson GE, Page RE Jr (1988) Genetic determination of guarding and undertaking in honey bee colonies. Nature 333:356–358Google Scholar
  25. Ruttner F (1988) Biogeography and taxonomy of honeybees. Springer, BerlinGoogle Scholar
  26. Seeley TD, Seeley RH, Akratanakul P (1982) Colony defence strategies of the honey bees in Thailand. Ecol Monogr 52:43–63Google Scholar
  27. Shaskolsky DV (1976) Polyandry —a defending factor of the colony against a great number of lethal eggs. In: Apimondia symposium on bee biology, Moscow, USSR: Genetics selection and reproduction in the honey bee. Apimondia, Bucharest, pp 67–71Google Scholar
  28. Sheppard WS, McPheron BA (1991) Ribosomal DNA diversity in Apidae. In: Smith (ed) Diversity in the genus Apis. Westview Press, Boulder, pp 89–102Google Scholar
  29. Sherman PW, Seeley TD, Reeve HK (1988) Parasites, pathogens and polyandry in the social Hymenoptera. Am Nat 131:602–610Google Scholar
  30. Shykoff JA, Schmid-Hempel P (1990) Genetic relatedness and eusociality: parasite mediated selection on the genetic composition of groups. Behav Ecol Sociobiol 28:371–376Google Scholar
  31. Smith CAB (1986) Chi-squared tests with small numbers. Ann Hum Genet 50:163–167Google Scholar
  32. Steel RGD, Torrie JH (1980) Principles and procedures of statistics. McGraw Hill, TokyoGoogle Scholar
  33. Stuart RJ, Page RE (1991) Genetic component to division of labor among workers of a Leptothoracine ant. Naturwissenschaften 78:375–377Google Scholar
  34. Woyke J (1963) What happens to diploid drone larvae in a honey bee colony? J Apic Res 2:73–75Google Scholar
  35. Woyke J (1986) Sex determination. In: Rinderer TE (ed) Bee genetics and breeding. Academic Press, Orlando, pp 91–120Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Benjamin P. Oldroyd
    • 1
  • H. Allen Sylvester
    • 1
  • Siriwat Wongsiri
    • 2
  • Thomas E. Rinderer
    • 1
  1. 1.Honey-Bee BreedingGenetics and Physiology Research Laboratory, USDA-ARSBaton RougeUSA
  2. 2.Bee Biology Research Unit, Faculty of ScienceChulalongkorn UniversityBangkokThailand

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