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The program ASNO for computer-supported collection of NOE upper distance constraints as input for protein structure determination

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Summary

A new program, ASNO (‘ASsign NOes’), for computer-supported NOE cross-peak assignments is described. ASNO is used for structure refinement in several rounds of NOESY cross-peak assignments and 3D structure calculations, where the preliminary structures are used as a reference to resolve ambiguities in NOE assignments which are otherwise based on the chemical shifts available from the sequence-specific resonance assignments. The practical use of ASNO for proteins is illustrated with the structure determination of Dendrotoxin K from Dendroaspis polylepis polylepis.

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Abbreviations

Toxin K:

dendrotoxin K (or ‘trypsin inhibitor homologue K’) from the venom of the black mamba Dendroaspis polylepis polylepis

NOE:

nuclear Overhauser effect

NOESY:

NOE spectroscopy

REDAC:

use of redundant dihedral angle constraints

RMSD:

root-mean-square deviation

References

  • Anil-Kumar, Ernst, R.R. and Wüthrich, K. (1980) Biochem. Biophys. Res. Commun., 95, 1–6.

    Google Scholar 

  • Berndt, K.D., Güntert, P. and Wüthrich, K. (1993) J. Mol. Biol., in press.

  • Eccles, C., Güntert, P., Billeter, M. and Wüthrich, K. (1991) J. Biomol. NMR, 1, 111–130.

    Google Scholar 

  • Ferrin, T.E., Huang, C.C., Jarvis, L.E. and Langridge, R. (1988) J. Mol. Graphics, 6, 13–27.

    Google Scholar 

  • Güntert, P. and Wüthrich, K. (1991) J. Biomol. NMR, 1, 447–456.

    Google Scholar 

  • Güntert, P., Braun, W. and Wüthrich, K. (1991a) J. Mol. Biol., 217, 517–530.

    Google Scholar 

  • Güntert, P., Braun, W., Billeter, M. and Wüthrich, K. (1989) J. Am. Chem. Soc., 111, 3997–4004.

    Google Scholar 

  • Güntert, P., Qian, Y.Q., Otting, G., Müller, M., Gehring, W. and Wüthrich, K. (1991b) J. Mol. Biol., 217, 531–540.

    Google Scholar 

  • McLachlan, A.D. (1979) J. Mol. Biol., 128, 49–79.

    Google Scholar 

  • Szyperski, T., Güntert, P., Otting, G. and Wüthrich, K. (1992) J. Magn. Reson., 99, 552–560.

    Google Scholar 

  • Williamson, M.P., Havel, T.F. and Wüthrich, K. (1985) J. Mol. Biol., 182, 295–315.

    Google Scholar 

  • Wüthrich, K. (1986) NMR of Proteins and Nucleic Acids, Wiley, New York.

    Google Scholar 

  • Wüthrich, K., Billeter, M. and Braun, W. (1983) J. Mol. Biol., 169, 949–961.

    Google Scholar 

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Author notes

  1. Kurt D. Berndt

    Present address: Karolinska institutet, Institutionen för Medicinsk fysik, Stockholm, Sweden

Authors and Affiliations

  1. Eidgenössische Technische Hochschule-Hönggerberg, Institut für Molekularbiologie und Biophysik, CH-8093, Zürich, Switzerland

    Peter Güntert, Kurt D. Berndt & Kurt Wüthrich

Authors
  1. Peter Güntert
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  2. Kurt D. Berndt
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  3. Kurt Wüthrich
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Güntert, P., Berndt, K.D. & Wüthrich, K. The program ASNO for computer-supported collection of NOE upper distance constraints as input for protein structure determination. J Biomol NMR 3, 601–606 (1993). https://doi.org/10.1007/BF00174613

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  • DOI: https://doi.org/10.1007/BF00174613

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