Abstract
Alkaline phosphatases (APs) are a family of cell surface glycoproteins that are expressed in a variety of tissues. Their physiologcial functions are still unclear. Three different AP genes have been found to be expressed in mice, and AP cloned from the placenta is of the tissue non-specific (TNAP) type. We have in investigated the location of TNAP mRNA and active AP in mature mouse placenta, using in situ hybridization and enzyme histochemistry on serial sections. Digital image analysis was used to estimate relative amounts of TNAP mRNA. Tissue non-specific alkaline phosphatase messenger was detected only in the placental labyrinth, whereas active AP was present both in the labyrinth and in a zone of cells at the margin of the decidua basalis, bordering the myometrium and the metrial gland. This latter location of AP activity has not been described previously. The AP-positive zone of the decidua had a condensed appearance and a central defect in the zone was visible on sections taken from the middle of the placenta. No TNAP messenger was found in the zone of AP-positive decidual cells.
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References
Angerer LM, Angerer RC (1991) In situ hybridization — a guided tour. Toxicol Methods 1:2–29
Arceci RJ, Shanahan F, Stanley ER, Pollard JW (1989) Temporal expression and location of colony-stimulating factor 1 (CSF-1) and its receptor in the female reproductive tract are consistent with CSF-1-regulated placental development. Proc Natl Acad Sci USA 86:8818–8822
Bridgman J (1948a) A morphological study of the development of the placenta of the rat. I. An outline of the development of the placenta of the white rat. J Morphol 1:61–86
Bridgman J (1948b) A morphological study of the development of the placenta of the rat. II. An histological and cytological study of the development of the chorioallantoic placenta of the white rat. J Morphol 2:195–224
Campbell WJ, Larsen D, Deb S, Kwok SCM, Soares MJ (1991) Expression of alkaline phosphatase in differentiated rat labyrinthine trophoblast tissue. Placenta 12:227–237
Clayden EC (1962) Practical section cutting and staining. Churchill, London
Conover WJ (1980) Practical nonparametric statistics. Wiley, USA
Davies J, Glasser SR (1968) Histological and fine structural observations on the placenta of the rat. Acta Anat 69:542–608
Friedrich F (1964) Die Entwicklung der sogenannten Dottersackdivertikel in der Placenta der weißen Maus. Z Anat Entwicklungsgesch 124:153–170
Glazier JD, Jones CJP, Sibley P (1990) Preparation of plasma membrane vesicles from the rat placenta at term and measurement of Na+ uptake. Placenta 11:451–463
Goldstein DJ, Rogers CE, Harris H (1980) Expression of alkaline phosphatase loci in mammalian tissues. Proc Natl Acad Sci USA 77:2857–2860
Hahnel AC, Rappolee DA, Millan JL, Manes T, Ziomek CA, Theososiou NG, Werb Z, Pedersen RA, Schultz GA (1990) Two alkaline phosphatase genes are expressed during early development in the mouse embryo. Development 110:555–564
Harris H (1989) The human alkaline phosphatases: what we know and what we don't know. Clin Chim Acta 186:133–150
Jollie WP (1964) Fine structural changes in placental labyrinth of the rat with increasing gestational age. J Ultrastruct Res 10:27–47
Jollie WP (1965) Fine structural changes in the junctional zone of the rat placenta with increasing gestational age. J Ultrastruct Res 12:420–438
Makiya R, Stigbrand T (1992a) Placental alkaline phosphatase has a binding site for the human immunoglobulin-G Fc portion. Eur J Biochem 205:341–345
Makiya R, Stigbrand T (1992b) Placental alkaline phosphatase is related to human IgG internalization in HEp2 cells. Biochem Biophys Res Commun 182:624–630
Makiya R, Thornell LE, Stigbrand T (1992) Placental alkaline phosphatase, a GPI-anchored protein, is clustered in clathrin-coated vesicles. Biochem Biophys Res Commun 183:803–808
McComb RB, Bowers GN, Posen S (1979) Alkaline Phosphatases. Plenum Press, New York
Metz J, Heinrich D, Forssman WG (1976) Ultrastructure of the labyrinth in the rat fall-term placenta. Anat Embryol 149:123–148
Millán JL (1990) Oncodevelopmental alkaline phosphatases: in search for a function. In: Ogita Z-I, Markert CL (eds) Isozymes: structure, function, and use in biology and medicine. Wiley-Liss, New York, pp 453–475
Müntener M, Hsu YC (1977) Development of trophoblast and placenta of the mouse. Acta Anat 98:241–252
Pollard JW, Bartocci A, Arceci R, Orlofsky A, Ladner MB, Stanley RE (1987) Apparent role of the macrophage growth factor, CSF-1, in placental development. Nature 330:484–486
Pollard JW, Jahan M, Butterworth PJ (1990) Characterization and expression of uterine and placental alkaline phosphatases in the mouse. J Reprod Fertil 89:735–742
Redline RW, Lu CY (1989) Localization of fetal major histocompatibility complex antigens and maternal leukocytes in murine placenta. Lab Invest 61:27–36
Rossant J, Croy BA (1985) Genetic identification of tissue origin of cellular populations within the mouse placenta. J Embryol Exp Morphol 86:177–189
Rugh R (1991) The mouse. Its reproduction and development. Oxford University Press, Oxford
Sambrook J, Fritsch E-, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor New York
Soares MJ (1987) Developmental changes in the intraplacental distribution of placental lactogen and alkaline phosphatase in the rat. J Reprod Fertil 79:93–98
Terao M, Mintz B (1987) Cloning and characterization of a cDNA coding for mouse placental alkaline phosphatase. Proc Natl Acad Sci USA 84:7051–7055
Theiler K (1972) The house mouse. Springer, Berlin Heidelberg New York
Tsonis PA, Argraves WS, Millan JL (1988) A putative functional domain of human placental alkaline phosphatase predicted from sequence comparison. Biochem J 254:623–624
Vittur F, Stagni N, Moro L, Bernard B (1984) Alkaline phosphatase binds to collagen; a hypothesis on the mechanism of extravesicular mineralisation in epiphyseal cartilage. Experentia 40:836–837
Weinreb M, Shinar D, Rodan GA (1990) Different pattern of alkaline phosphatase, osteopontin, and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Min Res 5:831–842
Welsh AO, Enders AC (1987) Trophoblast-decidual cell interactions and establishment of maternal blood circulation in the parietal yolk sac placenta of the rat. Anat Rec 217:203–219
Wilkinson DG, Green J (1990) In situ hybridisation and the three dimensional reconstruction of serial sections. In: Copp AJ, Cockroft DL (eds) Postimplantation mammalian embryos — a practical approach. Oxford University Press, Oxford, pp 155–171
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Johansson, S., Wide, M., Young, E. et al. Expression of alkaline phosphatase in the mature mouse placenta visualized by in situ hybridization and enzyme histochemistry. Anat Embryol 187, 409–414 (1993). https://doi.org/10.1007/BF00174416
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DOI: https://doi.org/10.1007/BF00174416