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Uptake of bacteriophage λ DNA in Escherichia coli by electroporation: An alternative to in vitro packaging

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Biotechnology Techniques

Summary

By using a high field strength DC pulse of 15 kV/cm and a pulse duration of 5 ms for the transfection of E. coli by bacteriophage λ DNA, we obtained efficiencies of 1.1 × 106 (pfu/μg bacteriophage λ, DNA). This represents a 100-fold improvement over the traditional CaCl2/heat shock method and is a viable alternative to the more costly in vitro packaging of recombinant bacteriophage λ DNA for the production of cDNA and genomic libraries.

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Authors and Affiliations

  1. Department of Biology, Montclair State University, 07043, Upper Montclair, NJ, USA

    John J. Gaynor & Martin G. Fox

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  1. John J. Gaynor
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  2. Martin G. Fox
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Gaynor, J.J., Fox, M.G. Uptake of bacteriophage λ DNA in Escherichia coli by electroporation: An alternative to in vitro packaging. Biotechnol Tech 10, 463–467 (1996). https://doi.org/10.1007/BF00174234

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  • DOI: https://doi.org/10.1007/BF00174234

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