Summary
Stability of cellulase was improved by modification with synthetic copolymers of polyoxyalkylene derivative and maleic anhydride. The modified cellulases under 50% degree of modification showed higher initial activities than did native cellulase. Modified cellulase showed excellent stability of activity against wide pH variation and insoluble solvent. Moreover, cellulase modified with hydrophobic copolymer could be dissolved in these solvents.
Similar content being viewed by others
References
Clanet, M., Durand, H. and Tiraby, G. (1988), Biotechnol. Bioeng., 32, 930–937.
Habeeb, A. F. S. F. (1966), Anal. Biotech., 14, 328–336.
Inada, Y., Takahashi, T., Yoshimoto, Y., Ajima, A., Matsushima, A. and Saito, Y. (1986), Trends Biotechnol. 4, 190–194.
Mandels, M., Andreotti, R., and Roche, C. (1976), Biotechnol. Bioeng. Symp. 6, 21–26.
McCord, J. M. and Fridorich, I. (1969), J. Biol. Chem. 244, 6049–6053
Miller, G. L. (1959), Anal. Chem., 31, 426–428.
Nishio, T., Takahashi, T., Yoshimoto, Y., Kodera, Y., Saito, Y., and Inada, Y.,(1987), Biotechnol. Lett., 9, 187–190.
Park, J. W., Takahata, Y., Kajiuchi, T. and Akehata, T. (1992), Biotechnol. Bioeng.,39, 117–120.
Rivers, D. B. and Emert, G. H. (1988), Biotechnol. Bioeng., 31, 278–281.
Tanaka, M. and Matsuno, R.,(1985), Enzy. Microbiol. Techmol. 7, 197–203.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Park, J.W. Improvement of cellulase stability by the covalent modification of copolymer of polyalkylene derivative. Biotechnol Tech 10, 457–462 (1996). https://doi.org/10.1007/BF00174233
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00174233