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An improved procedure for the recovery of pancreatic enzymes with enhanced activity

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Biotechnology Techniques

Summary

Pancreatic enzymes were recovered with enhanced activity by a newly designed extraction process. The protease activity was increased to the extent of 65% by using MES buffer, 64mM and duodenum enzyme extracts containing enterokinase at autolysis stage. The decrease in amylase activity due presumably to proteolytic degradation was effectively prevented by treating the enzyme solution with calcium prior to the autolysis.

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Authors and Affiliations

  1. Biochemical Engineering Laboratory, Hanhyo Institutes of Technology, San 6, Siheung-City, Kyungki-Do, South Korea

    Moo Heon Kang & Jeewon Lee

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  1. Moo Heon Kang
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  2. Jeewon Lee
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Kang, M.H., Lee, J. An improved procedure for the recovery of pancreatic enzymes with enhanced activity. Biotechnol Tech 10, 419–424 (1996). https://doi.org/10.1007/BF00174226

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  • DOI: https://doi.org/10.1007/BF00174226

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