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Rapid and simple analysis of poly-β-hydroxybutyrate content by capillary isotachophoresis

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Biotechnology Techniques

Summary

We present a rapid method for the direct analysis of poly-β-hydroxybutyrate (PHB) content in the soil bacteria Alcaligenes eutrophus. PHB from the fresh cells was converted by sulfuric acid to the crotonic acid and measured by capillary isotachophoresis after the neutralization by CaCO3. The method can be used for rapid and routine monitoring of the fermentation processes in samples containing 0.001 to 20 mg of PHB.

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Authors and Affiliations

  1. Institute of Biotechnology Faculty of Chemical Technology, Slovak Technical University, Radlinského 9, SK-812 37, Bratislava, Slovakia

    Pavol Sulo, Antónia Propperová & Ivan Bašnák

  2. Department of Microbiology, Biochemistry and Biology Faculty of Chemical Technology, Slovak Technical University, Radlinského 9, SK-812 37, Bratislava, Slovakia

    Daniela Hudecová

Authors
  1. Pavol Sulo
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  2. Daniela Hudecová
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  3. Antónia Propperová
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  4. Ivan Bašnák
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Sulo, P., Hudecová, D., Propperová, A. et al. Rapid and simple analysis of poly-β-hydroxybutyrate content by capillary isotachophoresis. Biotechnol Tech 10, 413–418 (1996). https://doi.org/10.1007/BF00174225

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  • DOI: https://doi.org/10.1007/BF00174225

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