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Isolation and random amplified polymorphic DNA analysis of genomic DNA from soybean embryos

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Biotechnology Techniques

Summary

An isolation procedure was developed for the extraction of genomic DNA and Random Amplified Polymorphic DNA (RAPD) analysis using individual soybean embryos. This procedure can be used to quickly and efficiently isolate DNA from a large number of individuals. DNA isolations were analyzed for total yield, integrity, and usefulness as a template in RAPD analysis.

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Author notes

  1. Paul N. McMahon & Marla S. McIntosh

    Present address: H.J. Patterson Hall, University of Maryland, 20742, College Park, Maryland, USA

Authors and Affiliations

  1. Department of Agronomy, University of Maryland, College Park, USA

    Paul N. McMahon & Marla S. McIntosh

Authors
  1. Paul N. McMahon
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  2. Marla S. McIntosh
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McMahon, P.N., McIntosh, M.S. Isolation and random amplified polymorphic DNA analysis of genomic DNA from soybean embryos. Biotechnol Tech 10, 407–412 (1996). https://doi.org/10.1007/BF00174224

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  • DOI: https://doi.org/10.1007/BF00174224

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