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Increased production of β-cyclodextrin using an aqueous two-phase system

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Biotechnology Techniques

Summary

β-Cyclodextrin(β-CD) was produced by cyclodextrin glycosyltransferase(CGTase) in aqueous two-phase system. β-CD production from soluble starch was catalyzed by CGTase in dextran-rich bottom phase, and the β-CD produced was transferred to PEG-rich top phase in aqueous two-phase system, composed of 7% (w/w) polyethylene glycol(Mr 20,000) and 10% (w/w) dextran(Mr 38,900). Partition coefficients of β-CD and CGTase were 1.5 and 0.25, respectively. The total productivity of β-CD in aqueous two-phase system was about 3 times of that in dextran phase.

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References

  • Bollag, D.M. and Edelstein, S.J. (1991). Protein Methods, pp. 50–55, New York: Wiley-Liss.

    Google Scholar 

  • Horikoshi, K. (1979). Process Biochemistry, 26–30.

  • Kaneko, T., Kato, T., Nakamura, N., and Horikoshi, K. (1987). J. Jpn Soc. Sci., 34(1), 45–48.

    Google Scholar 

  • King, R.S. (1992). Aqueous two-phase partitioning in biotechnology, in: Polymer Applications for Biotechnology,Soane, D.S. ed., pp. 204–243, New Jersey: Prentice-Hall.

    Google Scholar 

  • Lee, Y.H., Lee, S.H., and Han, I.K. (1991). Kor. J. Appl. Microbiol. Biotechnol., 19(2), 171–178.

    Google Scholar 

  • Makela, M.J., and Korpela, T.K. (1988). J. Agric. Food Chem., 36, 83–88.

    Google Scholar 

  • Nagotomo, S. (1985). Chemical Economy & Engineering Review, 17(190), 28–34.

    Google Scholar 

  • Szejtli, J. (1989). Tibtech., 7, 170–174.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biological Engineering, College of Engineering, Inha University, Inchon, South Korea

    Kyung-Hyun Min, Woo-Jin Chang & Yoon-Mo Koo

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  1. Kyung-Hyun Min
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  2. Woo-Jin Chang
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  3. Yoon-Mo Koo
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Min, KH., Chang, WJ. & Koo, YM. Increased production of β-cyclodextrin using an aqueous two-phase system. Biotechnol Tech 10, 395–400 (1996). https://doi.org/10.1007/BF00174222

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  • DOI: https://doi.org/10.1007/BF00174222

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